Immunofluorescent analysis of NRF2 (green) HEK293T cells. Cells fixed with 4% formaldehyde were permeabilized and blocked with 1X PBS containing 5% BSA and 0.3% Triton X-100 for 1 hour at room temperature. Cells were probed with an NRF2 polyclonal antibody (Product # PA5-27882) at a dilution of 1:100 overnight at 4°C in 1X PBS containing 1% BSA and 0.3% Triton X-100, washed with 1X PBS, and incubated with a fluorophore-conjugated goat anti-rabbit IgG secondary antibody at a dilution of 1:200 for 1 hour at room temperature. Nuclei (blue) were stained with DAPI. Images were taken on a Leica DM1000 microscope at 40X magnification. Data courtesy of the Innovators Program.
|Tested species reactivity||Human, Mouse|
|Published species reactivity||Zebrafish, Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant fragment corresponding to a region within amino acids 108 and 413 of Human NRF2|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7, with 20% glycerol|
|Contains||0.025% ProClin 300|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|ChIP assay (ChIP)||Assay-dependent|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100-1:1000|
|Western Blot (WB)||Assay-Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA5-27882 targets NRF2 in IF, ChIP, IHC (P), and WB applications and shows reactivity with Human and mouse samples.
The PA5-27882 immunogen is recombinant fragment corresponding to a region within amino acids 108 and 413 of Human NRF2.
NFE2 (MIM 601490), NFE2L1 (MIM 163260), and NFE2L2 comprise a family of human genes encoding basic leucine zipper (bZIP) transcription factors. They share highly conserved regions that are distinct from other bZIP families, such as JUN (MIM 165160) and FOS (MIM 164810), although remaining regions have diverged considerably from each other (Chan et al., 1995 [PubMed 7868116]).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Sodium fluoride affects zebrafish behaviour and alters mRNA expressions of biomarker genes in the brain: Role of Nrf2/Keap1.
PA5-27882 was used in western blot to elucidate the molecular mechanism of fluoride-induced neurotoxicity using zebrafish
|Mukhopadhyay D,Priya P,Chattopadhyay A||Environmental toxicology and pharmacology (40:352)||2015|
Acetyl-l-carnitine prevents homocysteine-induced suppression of Nrf2/Keap1 mediated antioxidation in human lens epithelial cells.
PA5-27882 was used in western blot to prevent homocysteine-induced demethylation by administering acetyl-l-carnitine to human lens epithelial cells.
|Yang SP,Yang XZ,Cao GP||Molecular medicine reports (12:1145)||2015|