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NRF2/NFE2L2 Polyclonal Antibody

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NRF2/NFE2L2 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human liver cancer tissue slide using 16396-1-AP (NRF2, NFE2L2 Antibody) at dilution of 1:200 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

NRF2/NFE2L2 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human ovary tumor tissue slide using 16396-1-AP ( NRF2, NFE2L2 Antibody) at dilution of 1:200 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

NRF2/NFE2L2 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human colon cancer tissue slide using 16396-1-AP (NRF2, NFE2L2 Antibody) at dilution of 1:200 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

NRF2/NFE2L2 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human colon cancer tissue slide using 16396-1-AP (NRF2, NFE2L2 Antibody) at dilution of 1:200 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

NRF2/NFE2L2 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human liver cancer tissue slide using 16396-1-AP (NRF2, NFE2L2 Antibody) at dilution of 1:200 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

NRF2/NFE2L2 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human ovary tumor tissue slide using 16396-1-AP (NRF2, NFE2L2 Antibody) at dilution of 1:200 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

NRF2/NFE2L2 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using 16396-1-AP (NRF2, NFE2L2 Antibody) at dilution of 1:50 (under 40x lens).

NRF2/NFE2L2 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using 16396-1-AP (NRF2, NFE2L2 Antibody) at dilution of 1:50 (under 10x lens).

NRF2/NFE2L2 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human kidney tissue slide using 16396-1-AP (NRF2, NFE2L2 Antibody) at dilution of 1:50 (under 10x lens).

NRF2/NFE2L2 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human kidney tissue slide using 16396-1-AP (NRF2, NFE2L2 Antibody) at dilution of 1:50 (under 40x lens).

NRF2/NFE2L2 Antibody in Western Blot (WB) — DMSO treated HeLa cells were subjected to SDS PAGE followed by western blot with 16396-1-AP (NRF2, NFE2L2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

NRF2/NFE2L2 Antibody in Western Blot (WB) — Various lysates were subjected to SDS PAGE followed by western blot with 16396-1-AP (NRF2, NFE2L2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

NRF2/NFE2L2 Antibody in Flow Cytometry (Flow) — 1X10^6 MCF-7 cells were stained with 0.2ug NRF2, NFE2L2 antibody (16396-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L) with dilution 1:1500.

NRF2/NFE2L2 Antibody in Immunoprecipitation (IP) — IP result of anti-NRF2, NFE2L2 (IP:16396-1-AP, 3ug; Detection:16396-1-AP 1:300) with mouse kidney tissue lysate 4000ug.

Product Details

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

0.2 µg/1x10^6 cells

-

Immunofluorescence (IF)

Assay-dependent

View 12 publications 12 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:500

-

Immunoprecipitation (IP)

0.5-4.0 µg

View 2 publications 2 publications

Western Blot (WB)

1:500-1:2000

View 104 publications 104 publications

Immunohistochemistry (IHC)

-

View 15 publications 15 publications

Miscellaneous PubMed (Misc)

-

View 3 publications 3 publications

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Avian, Human, Mouse, Pig, Rat

Host / Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

NRF2, NFE2L2 Fusion Protein Ag9489

Conjugate

Unconjugated

Form

Liquid

Concentration

0.47 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.1% sodium azide

Storage conditions

-20°C

Target Information

Nfr1 (also designated TCF11), Nrf2 and Nfr3 are bZIP transcription factors that heterodimer with Maf proteins to bind MARE (Maf recognition elements) sequences. Nrf2 is widely expressed and is thought to translocate to the nucleus after treatment with xenobiotics and antioxidants, which stimulate its release from a repressor protein Keap1.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: E4TF1-60; E4TF1A; HEBP1; NF-E2-related factor 2; NFE2-related factor 2; NFT2; nrf2; NRF2A; nuclear factor erythroid 2-like 2; Nuclear factor erythroid 2-related factor 2; nuclear factor erythroid-derived 2-like 2; nuclear factor, erythroid 2-like 2; Nuclear factor, erythroid derived 2, like 2

Gene Aliases: AI194320; HEBP1; NFE2L2; NRF2

UniProt ID: (Human) Q16236, (Mouse) Q60795, (Rat) O54968

Entrez Gene ID: (Human) 4780, (Mouse) 18024, (Rat) 83619

Molecular Function: actin family cytoskeletal protein cytoskeletal protein hydrolase non-motor actin binding protein nucleic acid binding protease serine protease transcription factor

transcription from RNA polymerase II promoter inflammatory response proteasomal ubiquitin-independent protein catabolic process positive regulation of gene expression protein ubiquitination positive regulation of blood coagulation endoplasmic reticulum unfolded protein response cellular response to oxidative stress cellular response to drug positive regulation of transcription from RNA polymerase II promoter in response to stress positive regulation of transcription from RNA polymerase II promoter in response to oxidative stress PERK-mediated unfolded protein response cellular response to glucose starvation proteasome-mediated ubiquitin-dependent protein catabolic process cell redox homeostasis positive regulation of transcription from RNA polymerase II promoter regulation of embryonic development positive regulation of glucose import cellular response to hydrogen peroxide cellular response to tumor necrosis factor cellular response to fluid shear stress cellular response to laminar fluid shear stress negative regulation of hematopoietic stem cell differentiation negative regulation of oxidative stress-induced intrinsic apoptotic signaling pathway positive regulation of ER-associated ubiquitin-dependent protein catabolic process negative regulation of hydrogen peroxide-induced cell death positive regulation of glutathione biosynthetic process regulation of removal of superoxide radicals negative regulation of endothelial cell apoptotic process positive regulation of reactive oxygen species metabolic process transcription, DNA-templated regulation of transcription, DNA-templated response to endoplasmic reticulum stress positive regulation of transcription, DNA-templated negative regulation of cell death

Process(es)

transcription regulatory region sequence-specific DNA binding RNA polymerase II distal enhancer sequence-specific DNA binding RNA polymerase II activating transcription factor binding transcriptional activator activity, RNA polymerase II distal enhancer sequence-specific binding transcription cofactor binding DNA binding transcription factor activity, sequence-specific DNA binding protein binding protein domain specific binding transcription regulatory region DNA binding sequence-specific DNA binding protein heterodimerization activity