Proteintech

NRF2/NFE2L2 Polyclonal Antibody

Product Image Gallery

NRF2/NFE2L2 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human liver cancer tissue slide using 16396-1-AP (NRF2, NFE2L2 Antibody) at dilution of 1:200 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

NRF2/NFE2L2 Antibody in Western Blot (WB) — DMSO treated HeLa cells were subjected to SDS PAGE followed by western blot with 16396-1-AP (NRF2, NFE2L2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

NRF2/NFE2L2 Antibody in Flow Cytometry (Flow) — 1X10^6 MCF-7 cells were stained with 0.2ug NRF2, NFE2L2 antibody (16396-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L) with dilution 1:1500.

NRF2/NFE2L2 Antibody in Immunoprecipitation (IP) — IP result of anti-NRF2, NFE2L2 (IP:16396-1-AP, 3ug; Detection:16396-1-AP 1:300) with mouse kidney tissue lysate 4000ug.

Product Details

Tested Applications

Dilution

Flow Cytometry (Flow)

0.2 µg/1x10^6 cells

Immunofluorescence (IF)

Assay-dependent

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:500

Immunoprecipitation (IP)

0.5-4.0 µg

Western Blot (WB)

1:500-1:2000

Published Applications

Immunohistochemistry (IHC)

See 15 publications below

Western Blot (WB)

See 104 publications below

Miscellaneous PubMed (MISC)

See 3 publications below

Immunofluorescence (IF)

See 12 publications below

Immunoprecipitation (IP)

See 2 publications below

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Avian, Human, Mouse, Pig, Rat

Host / Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

NRF2, NFE2L2 Fusion Protein Ag9489

Conjugate

Unconjugated

Form

Liquid

Concentration

0.47 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.1% sodium azide

Storage conditions

-20°C

Target Information

Nrf2 (nuclear factor erythroid 2-related factor 2) is a transcription activator which plays a key role in protecting the cell from oxidative stress and inflammation. It is primarily localized in the cytoplasm under normal conditions, but under conditions of oxidative stress, Nrf2 migrates to the nucleus to upregulate expression of genes with ARE (antioxidant response elements) in the promoter region. The upregulated genes include those involved in cellular protection, drug transport, detoxification, and anti-oxidant defense. The protein is ubiquitously expressed in various tissues, with the highest levels found in kidney, muscle, lung, heart, liver, and brain.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: HEBP1; NF E2 related factor 2; NF-E2-related factor 2; NFE2 related factor 2; NFE2-related factor 2; NFE2L2; NRF2; nuclear factor erythroid 2-like 2; Nuclear factor erythroid 2-related factor 2; nuclear factor erythroid-derived 2-like 2; nuclear factor, erythroid 2-like 2; Nuclear factor, erythroid derived 2, like 2

Gene Aliases: AI194320; HEBP1; NFE2L2; Nrf-2; NRF2

UniProt ID: (Human) B4E338, (Mouse) Q60795, (Rat) O54968

Entrez Gene ID: (Human) 4780, (Mouse) 18024, (Rat) 83619

Molecular Function: actin family cytoskeletal protein cytoskeletal protein hydrolase non-motor actin binding protein nucleic acid binding protease serine protease transcription factor