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Western blot analysis of HeLa cell extract using PA5-19427, NSD3 primary antibody at a dilution of 1/500 (lane 1). PA5-19427 staining of 293 cell extract (lane 2). PA5-19427 staining of HeLa cell extract and blocking peptide (lane 3). PA5-19427 staining of 293 cell extract and blocking peptide (lane 4). Blot treated with a secondary HRP-conjugated Goat polyclonal anti-Rabbit antibody was used at a dilution of 1:3000.
|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide derived from residues 500 - 600 of Human NSD3.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.4, with 1% BSA|
|Contains||0.02% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunocytochemistry (ICC)||5 µg/ml|
|Immunofluorescence (IF)||5 µg/ml|
|Immunohistochemistry (Paraffin) (IHC (P))||1:50-1:100|
|Western Blot (WB)||1 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA5-19427 targets NSD3 in ICC/IF, IHC (P), and WB applications and shows reactivity with human samples. For IHC staining, heat mediated antigen retrieval is recommended.
The PA5-19427 immunogen is synthetic peptide derived from residues 500 - 600 of Human NSD3.
PA5-19427 detects NSD3 which has a predicted molecular weight of approximately 72.6 kDa and 161 kDa.
This gene is related to the Wolf-Hirschhorn syndrome candidate-1 gene and encodes a protein with PWWP domains. The function of the protein has not been determined. Two alternatively spliced variants have been described.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
DC28, DKFZp667H044, FLJ20353, MGC126766, MGC142029, NSD3, pp14328, WHSC1-like 1 isoform 9 with methyltransferase activity to lysine; histone-lysine N-methyltransferase NSD3; nuclear receptor binding SET domain protein 3; nuclear SET domain-containing protein 3; protein whistle; WHSC1-like 1 isoform 9 with methyltransferase activity to lysine; WHSC1-like protein 1
DC28; NSD3; pp14328; WHISTLE; WHSC1L1