|Tested species reactivity||Chicken, Human, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||A synthetic peptide made to a C-terminal region of the murine numb protein sequence (between residues 600-653).|
|Purification||Antigen affinity chromatography|
|Storage buffer||tris glycine with 0.15M NaCl|
|Contains||0.05% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Western Blot (WB)||0.2-0.5 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
The target sequence has 90% sequence homology with rat.
Numb is an evolutionarily conserved membrane-associated protein. It exists in four isoforms that differ in two functional domains: a phosphotyrosine-binding (PTB) domain and a proline-rich region (PRR). It plays a role in the control of cell fate decisions during development and antagonizes the actions of Notch. Numb proteins appear to control the self-renewal of all neural progenitor cells and could possibly play similar roles in stem-cell maintenance in other tissues.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.