Immunofluorescent analysis of NUMB in HepG2 cells using a NUMB polyclonal antibody (Product # PA1-32452) at a dilution of 1:500 and detected using a Dylight 488 secondary antibody (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).
|Tested species reactivity||Human, Mouse, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to a C-terminal region of the murine numb protein sequence (between residues 600-653).|
|Storage buffer||tris glycine with 0.15M NaCl|
|Contains||0.05% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Western Blot (WB)||Assay dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-32452 detects Numb from mouse, human samples.
PA1-32452 has been successfully used in Western blot applications.
The PA1-32452 immunogen is: Synthetic peptide corresponding to a C-terminal region of the mouse numb protein sequence (between residues 600-653).
The protein encoded by this gene plays a role in the determination of cell fates during development. The encoded protein, whose degradation is induced in a proteasome-dependent manner by MDM2, is a membrane-bound protein that has been shown to associate with EPS15, LNX1, and NOTCH1. Four transcript variants encoding different isoforms have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.