Proteintech

NUMBL Polyclonal Antibody

View all (15) NUMBL antibodies

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NUMBL Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of ( -20°C Ethanol ) fixed HepG2 cells using 10111-1-AP (NUMBL antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

NUMBL Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of (-20°C Ethanol) fixed PC-12 cells using 10111-1-AP (NUMBL antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG (H+L).

NUMBL Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human brain tissue slide using 10111-1-AP ( NUMBL antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

NUMBL Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human brain tissue slide using 10111-1-AP ( NUMBL antibody) at dilution of 1:200 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

NUMBL Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human gliomas tissue slide using 10111-1-AP (NUMBL antibody at dilution of 1:50 (under 40x lens).

NUMBL Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human gliomas tissue slide using 10111-1-AP (NUMBL antibody at dilution of 1:50 (under 10x lens).

NUMBL Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human lung cancer using 10111-1-AP (NUMBL antibody) at dilution of 1:100 (under 10x lens).

NUMBL Antibody in Western Blot (WB) — Mouse spleen tissue were subjected to SDS PAGE followed by western blot with 10111-1-AP (NUMBL antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

NUMBL Antibody in Western Blot (WB) — Mouse liver tissue were subjected to SDS PAGE followed by western blot with 10111-1-AP (NUMBL antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

NUMBL Antibody in Western Blot (WB) — Mouse lung tissue were subjected to SDS PAGE followed by western blot with 10111-1-AP (NUMBL antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

NUMBL Antibody in Western Blot (WB) — Mouse brain tissue were subjected to SDS PAGE followed by western blot with 10111-1-AP ( NUMBL antibody at dilution of 1:2000 incubated at 4 degree celsius over night.

NUMBL Antibody in Western Blot (WB) — HeLa cells were subjected to SDS PAGE followed by western blot with 10111-1-AP (NUMBL antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

NUMBL Antibody in Western Blot (WB) — WB result of NUMBL antibody (10111-1-AP, 1:3000) with si-Control and si-NUMBL transfected HeLa cells.

NUMBL Antibody in Immunoprecipitation (IP) — IP result of anti-NUMBL (IP:10111-1-AP, 4ug; Detection:10111-1-AP 1:1000) with HeLa cells lysate 2800 ug.

Product Details

10111-1-AP

Applications	Tested Dilution	Publications
Western Blot (WB)	1:1,000-1:4,000	View 3 publications 3 publications
Immunohistochemistry (Paraffin) (IHC (P))	1:50-1:500	-
Immunocytochemistry (ICC/IF)	1:50-1:500	-
Immunoprecipitation (IP)	0.5-4.0 µg	-
Immunohistochemistry (IHC)	-	View 3 publications 3 publications

Product Specifications
Species Reactivity	Human, Mouse, Rat
Published species	Human, Mouse
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	NUMBL Fusion Protein Ag0156 (1-280 aa encoded by BC001794) View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.3 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.3, with 50% glycerol
Contains	0.02% sodium azide
Storage conditions	-20°C

Product Specific Information

Immunogen sequence: MNKLRQSLR RRKPAYVPEA SRPHQWQADE DAVRKGTCSF PVRYLGHVEV EESRGMHVCE DAVKKLKAMG RKSVKSVLWV SADGLRVVDD KTKDLLVDQT IEKVSFCAPD RNLDKAFSYI CRDGTTRRWI CHCFLALKDS GERLSHAVGC AFAACLERKQ RREKECGVTA AFDASRTSFA REGSFRLSGG GRPAEREAPD KKKAEAAAAP TVAPGPAQPG HVSPTPATTS PGEKGEAGTP VAAGTTAAAI PRRHAPLEQL VRQGSFRGFP ALSQKNSPFK R (1-280 aa encoded by BC001794)

Target Information

Plays a role in the process of neurogenesis. Required throughout embryonic neurogenesis to maintain neural progenitor cells, also called radial glial cells (RGCs), by allowing their daughter cells to choose progenitor over neuronal cell fate. Not required for the proliferation of neural progenitor cells before the onset of embryonic neurogenesis. Also required postnatally in the subventricular zone (SVZ) neurogenesis by regulating SVZ neuroblasts survival and ependymal wall integrity. Negative regulator of NF-kappa-B signaling pathway. The inhibition of NF-kappa-B activation is mediated at least in part, by preventing MAP3K7IP2 to interact with polyubiquitin chains of TRAF6 and RIPK1 and by stimulating the 'Lys-48'-linked polyubiquitination and degradation of TRAF6 in cortical neurons. [UniProt]

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Regulation of lung cancer metastasis by Klf4-Numb-like signaling.

Cancer research

Vaira V,Faversani A,Martin NM,Garlick DS,Ferrero S,Nosotti M,Kissil JL,Bosari S,Altieri DC

Mon Apr 15 00:00:00 EDT 2013

Regulation of lung cancer metastasis by Klf4-Numb-like signaling.

Cancer research

Vaira V,Faversani A,Martin NM,Garlick DS,Ferrero S,Nosotti M,Kissil JL,Bosari S,Altieri DC

Mon Apr 15 00:00:00 EDT 2013

Numb deletion in POMC-expressing cells impairs pituitary intermediate lobe cell adhesion, progenitor cell localization, and neuro-intermediate lobe boundary formation.

Molecular endocrinology (Baltimore, Md.)

Moran TB,Goldberg LB,Serviss SL,Raetzman LT

Sat Jan 01 00:00:00 EST 2011

Numb and Numbl are required for maintenance of cadherin-based adhesion and polarity of neural progenitors.

Nature neuroscience

Rasin MR,Gazula VR,Breunig JJ,Kwan KY,Johnson MB,Liu-Chen S,Li HS,Jan LY,Jan YN,Rakic P,Sestan N

Sun Jul 01 00:00:00 EDT 2007

Effects of testosterone supplementation on skeletal muscle fiber hypertrophy and satellite cells in community-dwelling older men.

The Journal of clinical endocrinology and metabolism

Sinha-Hikim I,Cornford M,Gaytan H,Lee ML,Bhasin S

Tue Aug 01 00:00:00 EDT 2006

Effects of testosterone supplementation on skeletal muscle fiber hypertrophy and satellite cells in community-dwelling older men.

The Journal of clinical endocrinology and metabolism

Sinha-Hikim I,Cornford M,Gaytan H,Lee ML,Bhasin S

Tue Aug 01 00:00:00 EDT 2006

Bioinformatics

Protein Aliases: numb homolog (Drosophila)-like; numb homolog-like; Numb-like protein; Numb-R; Numb-related protein

Gene Aliases: CAG3A; CTG3a; NBL; NUMB-R; NUMBL; NUMBLIKE; NUMBR; TNRC23

UniProt ID: (Human) Q9Y6R0, (Mouse) O08919, (Rat) A1L1I3

Entrez Gene ID: (Human) 9253, (Mouse) 18223, (Rat) 292732

Molecular Function: scaffold/adaptor protein

nervous system development axonogenesis cytokine-mediated signaling pathway protein metabolic process lateral ventricle development neuroblast division in subventricular zone adherens junction organization positive regulation of neurogenesis positive regulation of dendrite morphogenesis multicellular organism development neuroblast proliferation forebrain development

Process(es)

protein binding