Immunohistochemistry analysis of formalin-fixed, paraffin-embedded human skin (epidermis) stained using a NUP35 polyclonal antibody (Product # PA5-23338) at 10 ug/ml. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.
|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide (70 AGGSPPQPVVPAHKDK 85) of NUP35 protein.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS with 0.05% BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||5-10 µg/ml|
|Western Blot (WB)||1.0-2 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Nucleoporin Nup35 or Mitotic phosphoprotein 44 is a nuclear protein known to be present on nuclear pore complex and is tightly associated with the nuclear membrane and lamina. This RNA associated protein belongs to the Nup53 family and has a MPPN (mitotic phosphoprotein N' end) domain. It has a small number of scattered phenylalanine-glycine repeats, but not the large domains seen in other nucleoporins. Though the exact function of NUP35 is yet to be revealed, it may function as a component of the nuclear pore complex (NPC) which are collectively referred to as nucleoporins (NUPs). It also may play the role of both NPC structural components and of docking or interaction partners for transiently associated nuclear transport factors, and may also play an important part in the association of MAD1, a transcriptional repressor with the NPC. NUP35 is widely expressed in most of the tissues.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.