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Invitrogen
NUP62 Monoclonal Antibody (RL1)
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FIGURE: 1 / 7
NUP62 Antibody (MA1-071) in IHC (P)
Immunohistochemistry was performed on normal biopsies of deparaffinized Rat lymph node tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:200 with a mouse monoclonal a... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
MA1-071
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Amphibian,
Rat,
Yeast
Published species
Human,
Invertebrate,
Mouse,
Rat,
Yeast
Host/Isotype
Mouse
/ IgM
Class
Monoclonal
Type
Antibody
Clone
RL1
Immunogen
Pore complex-lamina fraction purified from rat liver nuclear envelopes.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Purification
PEG precipitation
Storage buffer
PBS
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
MA1-071 detects nuclear pore-O-linked glycoprotein from rat, amphibian and yeast.
MA1-071 has been successfully used in Western blot, immunofluorescence and immunoprecipitation procedures. By Western blot, this antibody detects up to eight different proteins from the nuclear pore complex (NPC) of approximately 210, 180, 145, 100, 63, 58, 54 and 45 kDa. Immunofluorescence staining of NPC O-linked glycoproteins with MA1-071 results in exclusive labeling of the NPC proteins on a wide variety of mammalian cells as well as S. cerevisiae and Xenopus. Labeling occurs exclusively at the NPC with most of the labeling at the cytoplasmic and nucleoplasmic margins.
Microinjected MA1-071 inhibits both protein import and RNA export in Xenopus oocytes.
The MA1-071 immunogen is pore complex-lamina fraction purified from rat liver nuclear envelopes.
Target Information
SDS3 is a subunit of the histone deacetylase.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: 62 kDa nucleoporin; DKFZp547L134; FLJ20822; FLJ43869; MGC841; nuclear pore glycoprotein 62; nucleoporin Nup62; nucleoporin p62; nucleoporin p62 homolog
Gene Aliases: Np62
UniProt ID: (Rat) P17955
Entrez Gene ID: (Rat) 65274
protein binding
phospholipid binding
structural constituent of nuclear pore
kinesin binding
receptor signaling complex scaffold activity
Hsp70 protein binding
SH2 domain binding
ubiquitin binding
macromolecular complex binding
thyroid hormone receptor binding
PTB domain binding
Hsp90 protein binding
RNA export from nucleus
protein import into nucleus
mitotic metaphase plate congression
centrosome cycle
mitotic centrosome separation
cell surface receptor signaling pathway
spermatogenesis
negative regulation of cell proliferation
negative regulation of epidermal growth factor receptor signaling pathway
regulation of protein import into nucleus
negative regulation of apoptotic process
negative regulation of programmed cell death
positive regulation of I-kappaB kinase/NF-kappaB signaling
negative regulation of MAP kinase activity
positive regulation of mitotic nuclear division
positive regulation of transcription, DNA-templated
negative regulation of Ras protein signal transduction
positive regulation of centriole replication
regulation of mitotic spindle organization
cellular senescence
centriole assembly
positive regulation of mitotic cytokinetic process
positive regulation of protein localization to centrosome
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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