Invitrogen

NUP62 Monoclonal Antibody (RL1)

Advanced Verification

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

1 Published Figure

12 References

View all (20) NUP62 antibodies

Datasheet

Protocols

Questions & Answers

Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

Product Image Gallery

NUP62 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry was performed on normal biopsies of deparaffinized Rat lymph node tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:200 with a mouse monoclonal antibody recognizing Nuclear Pore-O-Linked Glycoprotein (Product # MA1-071) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.

NUP62 Antibody in Western Blot (WB) — Western blot was performed using NUP62 Monoclonal Antibody (RL1) (Product # MA1-071) and a 53kDa band corresponding to NUP62 was observed across cell lines along with an uncharacterized band (*) at ~45 kDa. Whole cell extracts (30 µg lysate) of HEK-293 (Lane 1), THP-1 (Lane 2), HeLa (Lane 3) and PC-12 (Lane 4) were electrophoresed using NuPAGE™ 10% Bis-Tris Protein Gel (Product # NP0302BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

NUP62 Antibody in Immunocytochemistry, Immunohistochemistry (ICC/IF, IHC) — Figure 1. Construction and initial characterization of BAC transgenes. (A) BAC diagrams show positions of genes, regulatory elements, transposons with LacO arrays, vector sequences, and FISH probes (red, active genes; light blue, genes inactive in ES cells). (B) Deconvolved optical sections of EGFP-LacI signals (green) counterstained with DAPI (red) in different transgenic ES cell lines. (C) Deconvolved optical sections of EGFP-LacI (green), anti-nuclear pore immunostaining (red), and DAPI DNA staining (blue). Histograms of the shortest distances between EGFP-LacI spots and the nuclear periphery for one clone of each BAC type. Bars, 2 um.

NUP62 Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. HEK-293 cells were transfected with NUP62 siRNA and decrease in signal intensity was observed in western blot application using Anti-NUP62 Monoclonal Antibody (RL1) (Product # MA1-071). {KD}

Product Details

MA1-071

Applications	Tested Dilution	Publications
Western Blot (WB)	1:1,000	View 4 publications 4 publications
Immunohistochemistry (IHC)	1:200	View 2 publications 2 publications
Immunohistochemistry (Paraffin) (IHC (P))	-	View 1 publication 1 publication
Immunocytochemistry (ICC/IF)	-	View 4 publications 4 publications
Immunoprecipitation (IP)	Assay-dependent	View 1 publication 1 publication

Product Specifications
Species Reactivity	Amphibian, Rat, Yeast
Published species	Human, Invertebrate, Mouse, Rat, Yeast
Host/Isotype	Mouse / IgM
Class	Monoclonal
Type	Antibody
Clone	RL1
Immunogen	Pore complex-lamina fraction purified from rat liver nuclear envelopes.
Conjugate	Unconjugated
Form	Liquid
Purification	PEG precipitation
Storage buffer	PBS
Contains	0.05% sodium azide
Storage conditions	-20° C, Avoid Freeze/Thaw Cycles
RRID	AB_2157651

Product Specific Information

MA1-071 detects nuclear pore-O-linked glycoprotein from rat, amphibian and yeast.

MA1-071 has been successfully used in Western blot, immunofluorescence and immunoprecipitation procedures. By Western blot, this antibody detects up to eight different proteins from the nuclear pore complex (NPC) of approximately 210, 180, 145, 100, 63, 58, 54 and 45 kDa. Immunofluorescence staining of NPC O-linked glycoproteins with MA1-071 results in exclusive labeling of the NPC proteins on a wide variety of mammalian cells as well as S. cerevisiae and Xenopus. Labeling occurs exclusively at the NPC with most of the labeling at the cytoplasmic and nucleoplasmic margins.

Microinjected MA1-071 inhibits both protein import and RNA export in Xenopus oocytes.

The MA1-071 immunogen is pore complex-lamina fraction purified from rat liver nuclear envelopes.

Target Information

SDS3 is a subunit of the histone deacetylase.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Metabolic Labeling for the Visualization and Identification of Potentially O-GlcNAc-Modified Proteins.

Current protocols in chemical biology

Pedowitz NJ,Zaro BW,Pratt MR

MA1-071 was used in Western Blot to show that MCR labeling provides a powerful tool to discover where and when substrates are O-GlcNAc modified.

Mon Jun 01 00:00:00 EDT 2020

Cellular and Molecular Features of Developmentally Programmed Genome Rearrangement in a Vertebrate (Sea Lamprey: Petromyzon marinus).

PLoS genetics

Timoshevskiy VA,Herdy JR,Keinath MC,Smith JJ

MA1-071 was used in immunohistochemistry - paraffin section to study the sea lamprey (petromyzon marinus) vertebrate for molecular and cellular features of developmentally programmed genome rearrangement

Wed Jun 01 00:00:00 EDT 2016

Dynamic plasticity of large-scale chromatin structure revealed by self-assembly of engineered chromosome regions.

The Journal of cell biology

Sinclair P,Bian Q,Plutz M,Heard E,Belmont AS

MA1-071 was used in immunocytochemistry to investigate the folding of engineered chromosome regions

Mon Sep 06 00:00:00 EDT 2010

Dynamic plasticity of large-scale chromatin structure revealed by self-assembly of engineered chromosome regions.

The Journal of cell biology

Sinclair P,Bian Q,Plutz M,Heard E,Belmont AS

MA1-071 was used in immunocytochemistry to investigate the folding of engineered chromosome regions

Mon Sep 06 00:00:00 EDT 2010

Caenorhabditis elegans ortholog of a diabetes susceptibility locus: oga-1 (O-GlcNAcase) knockout impacts O-GlcNAc cycling, metabolism, and dauer.

Proceedings of the National Academy of Sciences of the United States of America

Forsythe ME,Love DC,Lazarus BD,Kim EJ,Prinz WA,Ashwell G,Krause MW,Hanover JA

MA1-071 was used in western blot to study the effect of O-GlcNAcase knockout in C. elegans.

Tue Aug 08 00:00:00 EDT 2006

Exportin-5, a novel karyopherin, mediates nuclear export of double-stranded RNA binding proteins.

The Journal of cell biology

Brownawell AM,Macara IG

MA1-071 was used in western blot to identify a new karyopherin exportin-5 and its cellular function.

Mon Jan 07 00:00:00 EST 2002

Identification and characterization of Caenorhabditis elegans gamma-tubulin in dividing cells and differentiated tissues.

Journal of cell science

Bobinnec Y,Fukuda M,Nishida E

MA1-071 was used in immunohistochemistry to identify and characterize gamma-tubulin in Caenorhabditis elegans cells and tissues

Wed Nov 01 00:00:00 EST 2000

A role for RanBP1 in the release of CRM1 from the nuclear pore complex in a terminal step of nuclear export.

The Journal of cell biology

Kehlenbach RH,Dickmanns A,Kehlenbach A,Guan T,Gerace L

MA1-071 was used in immunoprecipitation to study the role of importin beta in nuclear export.

Mon May 17 00:00:00 EDT 1999

Lamin-binding fragment of LAP2 inhibits increase in nuclear volume during the cell cycle and progression into S phase.

The Journal of cell biology

Yang L,Guan T,Gerace L

MA1-071 was used in immunocytochemistry to investigate the functional properties of lamin-binding fragment of LAP2 during the cell cycle and progression into S phase

Mon Dec 01 00:00:00 EST 1997

A conditional allele of the novel repeat-containing yeast nucleoporin RAT7/NUP159 causes both rapid cessation of mRNA export and reversible clustering of nuclear pore complexes.

The Journal of cell biology

Gorsch LC,Dockendorff TC,Cole CN

MA1-071 was used in immunocytochemistry to study how Rat7p regulates nucleocytoplasmic export of mRNA

Mon May 01 00:00:00 EDT 1995

Nuclear protein import is inhibited by an antibody to a lumenal epitope of a nuclear pore complex glycoprotein.

The Journal of cell biology

Greber UF,Gerace L

MA1-071 was used in immunocytochemistry to investigate the role of gp210 in the assembly and functions of nuclear pore complex

Wed Jan 01 00:00:00 EST 1992

O-linked glycoproteins of the nuclear pore complex interact with a cytosolic factor required for nuclear protein import.

The Journal of cell biology

Sterne-Marr R,Blevitt JM,Gerace L

MA1-071 was used in western blot to characterize a cytosolic factor indispensable for nuclear protein import

Wed Jan 01 00:00:00 EST 1992

Bioinformatics

Protein Aliases: 62 kDa nucleoporin; DKFZp547L134; FLJ20822; FLJ43869; MGC841; nuclear pore glycoprotein 62; Nuclear pore glycoprotein p62; Nucleoporin Nup62; nucleoporin p62

Gene Aliases: Np62; Nup62

UniProt ID: (Rat) P17955

Entrez Gene ID: (Rat) 65274

Molecular Function: transporter

Function(s)

transcription, DNA-templated cell surface receptor signaling pathway cell aging cell death negative regulation of cell proliferation protein transport negative regulation of epidermal growth factor receptor signaling pathway negative regulation of apoptotic process negative regulation of programmed cell death positive regulation of I-kappaB kinase/NF-kappaB signaling negative regulation of MAP kinase activity positive regulation of transcription, DNA-templated negative regulation of Ras protein signal transduction mRNA transport

Process(es)

protein binding structural constituent of nuclear pore receptor signaling complex scaffold activity SH2 domain binding ubiquitin binding thyroid hormone receptor binding PTB domain binding

NUP62 Monoclonal Antibody (RL1)

Product Details

MA1-071

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Purification

Storage buffer

Contains

Storage conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!