|Tested species reactivity||Human|
|Published species reactivity||Not Applicable|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant fragment corresponding to a region within amino acids 1 and 239 of Human Nectin 2|
|Purification||Antigen affinity chromatography|
|Storage buffer||0.1M tris glycine, pH 7, with 20% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100-1:1000|
|Western Blot (WB)||1:500-1:3000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Flow Cytometry (Flow)||See 1 publications below|
PA5-29757 targets Nectin 2 in IHC (P) and WB applications and shows reactivity with Human samples.
The PA5-29757 immunogen is recombinant fragment corresponding to a region within amino acids 1 and 239 of Human Nectin 2.
This gene encodes a single-pass type I membrane glycoprotein with two Ig-like C2-type domains and an Ig-like V-type domain. This protein is one of the plasma membrane components of adherens junctions. It also serves as an entry for certain mutant strains of herpes simplex virus and pseudorabies virus, and it is involved in cell to cell spreading of these viruses. Variations in this gene have been associated with differences in the severity of multiple sclerosis. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||
Differential expression of ligands for NKG2D and DNAM-1 receptors by epithelial ovarian cancer-derived exosomes and its influence on NK cell cytotoxicity.
PA5-29757 was used in flow cytometry, immunocytochemistry, and western blot to characterize the influence on NK cell cytotoxicity by differential expression of ligands for DNAM-1 and NKG2D receptors by epithelial ovarian cancer-derived exosomes
|Labani-Motlagh A,Israelsson P,Ottander U,Lundin E,Nagaev I,Nagaeva O,Dehlin E,Baranov V,Mincheva-Nilsson L||Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine (37:5455)||2016|