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Invitrogen
Nectin 3 Polyclonal Antibody
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FIGURE: 1 / 4
Nectin 3 Antibody (PA5-47441) in ICC/IF
Immunofluorescence analysis of Nectin 3 was performed using 70% confluent log phase Caco-2 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with Nectin 3 Goat Polyclonal Antibody (Product # PA5-47441) at 5 microgram/mL in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Rabbit anti-Goat IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 488 (Product # A-11078) ... View More
Product Details
PA5-47441
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
C. elegans
Host/Isotype
Goat
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Nectin-3 Leu56-Asp400
Conjugate
Form
Lyophilized
Concentration
0.2 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 5% trehalose
Contains
No Preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
In direct ELISAs, less than 65% cross-reactivity with recombinant mouse Nectin-3 is observed and less than 5% cross-reactivity with recombinant human (rh) Nectin-1, rhNectin-2, and rhNectin-4 is observed.
Reconstitute at 0.2 mg/mL in sterile PBS.
Target Information
Nectin-3 is a 83 kDa type I transmembrane glycoprotein. Nectin, originally isolated as poliovirus receptor-related protein (PRR), is a cell-cell adhesion molecule of the immunoglobulin supergene family. Nectins are calciumindependent immunoglobulin-like cell-cell adhesion molecules consisting of four members, nectin 1-4. Nectins homophilically and heterophilically trans-interact to form a variety of cell-cell junctions, including cadherin-based adherens junctions in epithelial cells and fibroblasts in culture, synaptic junctions in neurons, and Sertoli cell-spermatid junctions in testis, in cooperation with, or independently of, cadherins. Both nectin-2 and nectin-3 are ubiquitously expressed, whereas nectin-1 is abundantly expressed in brain. Nectin-2 and -3 are expressed in cells where cadherin is not expressed, such as blood cells and spermatids. All members of the nectin family have two or three splice variants. For nectin-3, three isoforms exist: nectin-3 alpha, -3 beta and -3 gamma, of which nectin-3 alpha is the largest. Nectin-3, also known as PRR3, is a transmembrane protein that is predominantly expressed in testis and placental tissues as well in many cell lines. Nectin interacts in vivo with both long and short isoforms of afadin, an actin binding protein, at cadherin-based cell-cell adherence junctions in various tissues and cell lines. Furthermore, the ectodomains of nectin-3 and CD155 (Poliovirus Receptor) have shown strong affinity to each other. Injection of antibody 103-A1 into lumen of seminiferous tubules leads to disruption of the actin filaments in Sertoli cells at the Sertoli-maturing spermatid ectoplasmic specialization and exfoliation of maturing spermatids form the seminiferous epithelium.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: CD113; CDw113; DKFZP566B0846; FLJ90624; nectin 3; Nectin cell adhesion molecule 3; Nectin-3; poliovirus receptor-related 3; Poliovirus receptor-related protein 3
Gene Aliases: CD113; CDW113; NECTIN-3; NECTIN3; PPR3; PRR3; PVRL3; PVRR3
UniProt ID: (Human) Q9NQS3
Entrez Gene ID: (Human) 25945
lens morphogenesis in camera-type eye
homophilic cell adhesion via plasma membrane adhesion molecules
heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules
spermatid development
cell recognition
fertilization
single organismal cell-cell adhesion
adherens junction organization
retina morphogenesis in camera-type eye
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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