|Tested species reactivity||Human, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||A 20 amino acid peptide from near the center of human neuritin.|
|Purification||Antigen affinity chromatography|
|Contains||0.02% sodium azide|
|Storage Conditions||Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C|
|Tested Applications||Dilution *|
|Immunocytochemistry (ICC)||20 ug/ml|
|Immunofluorescence (IF)||20 ug/ml|
|Immunohistochemistry (IHC)||5 ug/ml|
|Western Blot (WB)||5-10 ug/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
A suggested positive control is Daudi cell lysate.
PA5-20368 can be used with blocking peptide PEP-0482.
As the nervous system of a complex organism develops, it establishes functional networks through the growth and retraction of synaptic connections from growing axons and dendrites. This synaptic remodeling involves neuro-transmitter signaling, activation of neurotrophin receptors and alterations in gene expression. One such gene whose expression is increased by neural activity is neuritin, a GPI-anchored protein that is expressed in postmitotic differentiating neurons of the developing nervous system. Its expression is also induced by the neurotrophins BDNF and NT-3. Purified recombinant neuritin promotes neurite outgrowth and arborization in primary embryonic neuronal cultures, suggesting that neuritin may play a role as a downstream effector of activity-induced neurite outgrowth. More recent experiments have shown that neuritin is required for the androgen-induced axonal elongation in motor neurons and is upregulated following spinal cord injury, suggesting that neuritin may also play a role in survival and axonal regeneration.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.