|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||A synthetic peptide derived from the internal region of human NCAN|
|Purification||Antigen affinity chromatography|
|Storage buffer||Dulbecco's PBS, pH 7.4, with 50% glycerol, 150mM NaCl|
|Contains||0.02% sodium azide|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:500-1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
The lecticans are a family of chondroitin proteoglycans, including aggrecan, versican, neurocan and brevican, that contain a C-type lectin domain. Neurocan is a glycoprotein synthesized primarily by neurons, and its expression levels are highest during embryonic brain development and during the early postnatal period. Neurocan is a component of the extracellular matrix in the central nervous system that can bind to various other CNS matrix components, such as heparin, Tenascin-R, HB-GAM and NCAM, suggesting that it plays a role in axon guidance and neurite growth. Neurocan is a 1,257 amino acid, precursor protein in embryonic and neonatal rats that is proteolytically proc-essed in the adult CNS into an N-terminal fragment, which localizes to the cytoplasm of glial cells. Neurocan expression (including the re-expression of the neonatal isoform) increases significantly in adults in the glial scar resulting from cortical injury.
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