|Tested species reactivity||Human, Mouse, Rat|
|Published species reactivity||Not Applicable|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to residues W(1) Y K P A A G P H H Y S V G R A S G L L S S F H R F P S T(29) of mouse NPB protein.|
|Storage buffer||whole serum|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunofluorescence (IF)||1 µg/ml|
|Western Blot (WB)||1 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Miscellaneous PubMed (MISC)||See 1 publications below|
PA3-303 detects neuropeptide B from human, mouse and rat samples.
PA3-303 has been successfully used in Western blot and immunofluorescence procedures.
The PA3-303 immunogen is a synthetic peptide corresponding to residues W(1) Y K P A A G P H H Y S V G R A S G L L S S F H R F P S T(29) of mouse NPB protein.
Neuropeptide B is a endrogenous ligand of the orphan G protein coupled receptor GPR7. NPB and GPR7 exist in close proximity in the rat hypothalamus, and are ideally positioned to modulate neuroendocrine functions. NPB-immunoreactivity was enriched in many regions within the hypothalamus which also contained high levels of GPR7 mRNA including the ventrormedial hypothalamic nucleus, dorsomedial hypothalamic nucleus, arcuate nucleus, supraoptic retrochiasmatic nucleus, and in the area ventral to the zona incerta.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||
Immunofluorescent identification of neuropeptide B-containing nerve fibers and terminals in the rat hypothalamus.
PA3-303 was used in western blot and immunohistochemistry to investigate the localization of neuropeptide B in the rat hypothalamus
|Schulz S,Stumm R,Höllt V||Neuroscience letters (411:67)||2007|