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Neurturin Antibody (500-P102-100UG) in WB
Western Blot: To detect Human Neurturin by Western Blot analysis Neurturin Polyclonal Antibody (Product # 500-P102-1MG) can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human Neurturin is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Product Details
500-P102-100UG
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Rat
Host/Isotype
Rabbit
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived Recombinant Human Neurturin
Conjugate
Form
Lyophilized
Concentration
0.1-1.0 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient
RRID
AB_2929191
Product Specific Information
AA Sequence of recombinant protein: ARLGARPCGL RELEVRVSEL GLGYASDETV LFRYCAGACE AAARVYDLGL RRLRQRRRLR RERVRAQPCC RPTAYEDEVS FLDAHSRYHT VHELSARECA CV.
Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human Neurturin. Anti-Human Neurturin-specific antibody was purified by affinity chromatography employing an immobilized Human Neurturin matrix.
Sandwich ELISA: To detect Human Neurturin by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human Neurturin (500-P102Bt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human Neurturin.
Western Blot: To detect Human Neurturin by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human Neurturin is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Target Information
Glial cell line-derived neurotrophic factor (GDNF) plays key roles in the control of vertebrate neuron survival and differentiation. A novel neurotrophic factor was recently cloned from human and mouse and designated neurturin. Physiological responses to neurturin (NTN) require the presence of receptor tyrosine kinase RET and a novel glycosylphosphatidylinositol linked receptor NTNRalpha. The cDNAs encoding NTNRalpha from human, rat, chicken, and mouse have been cloned recently and termed GDNFR-b, Ret ligand 2 (RETL2) or TGF-beta-related neurotrophic factor receptor 2 (TrnR2) and nominated as GFR-a-2 recently. NTN binds to and forms a complex with GFR-a-2 and the Ret PTK and activates the RET receptor tyrosine kinase pathway. Both NTN and GDNF can activate the MAP kinase and phosphatidylinositol 3-kinase pathways and play a critical role in the development of many neuronal populations. Neurturin and GDNF define a new family of neurotrophic factors.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Neurturin; prepro-neurturin
Gene Aliases: NRTN; NTN
UniProt ID: (Human) Q99748
Entrez Gene ID: (Human) 4902
Performance Guarantee
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