Invitrogen

Neutrophil elastase Polyclonal Antibody

Advanced Verification

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

View all (28) Neutrophil elastase antibodies

Datasheet

Protocols

Questions & Answers

Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

Antibody Testing Data (3)
Advanced Verification (1)

Neutrophil elastase Antibody in Immunohistochemistry (IHC)

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Neutrophil elastase Antibody (PA5-84738) in IHC

Immunohistochemical analysis of Neutrophil elastase in human bone marrow and cerebral cortex tissues using a Neutrophil elastase polyclonal antibody (Product # PA5-84738). Corresponding RNA-seq data are presented for the same tissues.

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Neutrophil elastase Antibody — Relative expression in different tissues in IHC: Detection of differential expression levels of Neutrophil elastase demonstrates antibody specificity. Immunohistochemical analysis of Neutrophil elastase using anti-Neutrophil elastase Polyclonal Antibody (Product #PA5-84738), shows significant staining of Neutrophil elastase in bone marrow and shows minimal or weak staining in cerebral cortex tissues. The relative expression levels of Neutrophil elastase within each tissue is shown using RNA-Seq. {RE}

Product Details

PA5-84738

Applications

Tested Dilution

Publications

Immunohistochemistry (Paraffin) (IHC (P))

1:2500-1:5000

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein corresponding to Human ELANE

View immunogen

Conjugate

Unconjugated

Form

Liquid

Concentration

0.05 mg/mL

Purification

Anitgen affinity chromatography

Storage buffer

PBS, pH 7.2, with 40% glycerol

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

RRID

AB_2791888

Product Specific Information

Immunogen sequence: LVCNGLIHGI ASFVRGGCAS GLYPDAFAPV AQFVNWIDSI IQRSEDNPCP HP

Target Information

The neutrophil form of elastase is 218 amino acids long, with two asparagine-linked carbohydrate chains (see glycosylation). It is present in azurophil granules in the neutrophil cytoplasm. There appear to be two forms of neutrophil elastase, termed IIa and IIb.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Bone marrow serine protease; ELA2A; Elastase; elastase 2, neutrophil; Elastase-2; Elastase2; granulocyte-derived elastase; HLE; Human leukocyte elastase; leukocyte elastase; Medullasin; Neutrophil elastase; PMN elastase; polymorphonuclear elastase

Gene Aliases: ELA2; ELANE; GE; HLE; HNE; NE; PMN-E; SCN1

UniProt ID: (Human) P08246

Entrez Gene ID: (Human) 1991

Molecular Function: serine protease

Function(s)

negative regulation of transcription from RNA polymerase II promoter response to yeast acute inflammatory response to antigenic stimulus proteolysis cellular calcium ion homeostasis phagocytosis response to UV extracellular matrix disassembly protein catabolic process response to lipopolysaccharide defense response to bacterium positive regulation of MAP kinase activity negative regulation of growth of symbiont in host negative regulation of chemokine biosynthetic process negative regulation of interleukin-8 biosynthetic process positive regulation of interleukin-8 biosynthetic process positive regulation of smooth muscle cell proliferation negative regulation of inflammatory response positive regulation of immune response leukocyte migration negative regulation of chemotaxis neutrophil mediated killing of fungus

Process(es)

RNA polymerase II transcription corepressor activity protease binding endopeptidase activity serine-type endopeptidase activity protein binding heparin binding peptidase activity cytokine binding