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Nicastrin Polyclonal Antibody
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FIGURE: 1 / 3
Nicastrin Antibody (600-401-DB2) in ICC/IF
Immunofluorescence Microscopy of Nicastrin antibody. Cell Type: human brain cells. Fixation: 0.5% PFA. Antigen retrieval: not required. Primary antibody: Nicastrin antibody at 20 µg/mL for 1 hr at RT. Secondary antibody: Fluorescein rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: Nicastrin is located in the cell membrane. Staining: Nicastrin as red fluorescent signal.
Product Details
600-401-DB2
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Anti-Nicastrin antibody was prepared from whole rabbit serum produced by repeated immunizations with a 18 amino acid synthetic peptide from near the internal region of human Nicastrin.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Target Information
NCSTN (Nicastrin) is a gene that encodes a type I transmembrane glycoprotein that is an integral component of the multimeric gamma-secretase complex. The encoded protein cleaves integral membrane proteins, including Notch receptors and beta-amyloid precursor protein, and may be a stabilizing cofactor required for gamma-secretase complex assembly. The cleavage of beta-amyloid precursor protein yields amyloid beta peptide, the main component of the neuritic plaque and the hallmark lesion in the brains of patients with Alzheimer's disease; however, the nature of the encoded protein's role in Alzheimer's disease is not known for certain. Mutations in this gene are associated with familial acne inversa. A pseudogene of this gene is present on chromosome 21.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: anterior pharynx-defective 2; KIAA0253; Nicastrin; OTTHUMP00000031840; type 1 transmembrane protein; unnamed protein product
Gene Aliases: 9430068N19Rik; AA727311; Aph2; ATAG1874; D1Dau13e; KIAA0253; mKIAA0253; NCSTN; Nct; UNQ1874/PRO4317
UniProt ID: (Human) Q92542, (Mouse) P57716, (Rat) Q8CGU6
Entrez Gene ID: (Human) 23385, (Mouse) 59287, (Rat) 289231
myeloid cell homeostasis
proteolysis
membrane protein ectodomain proteolysis
Notch signaling pathway
protein processing
T cell proliferation
positive regulation of catalytic activity
beta-amyloid metabolic process
epithelial cell proliferation
dopamine receptor signaling pathway
glutamate receptor signaling pathway
Notch receptor processing
learning or memory
cerebellum development
central nervous system myelination
adult behavior
membrane protein intracellular domain proteolysis
beta-amyloid formation
amyloid precursor protein metabolic process
amyloid precursor protein biosynthetic process
positive regulation of amyloid precursor protein biosynthetic process
amyloid precursor protein catabolic process
neuron apoptotic process
cellular response to calcium ion
regulation of long-term synaptic potentiation
short-term synaptic potentiation
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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