Invitrogen

Nicastrin Polyclonal Antibody

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Nicastrin Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescence of Nicastrin in Human Brain cells with Nicastrin Polyclonal Antibody (Product # PA5-20303) at 20 µg/mL.

Nicastrin Antibody in Immunohistochemistry (IHC) — Immunohistochemistry of Nicastrin in human brain tissue with Nicastrin Polyclonal Antibody (Product # PA5-20303) at 5 µg/mL.

Nicastrin Antibody in Western Blot (WB) — Western Blot analysis of Nicastrin in mouse brain tissue lysate with Nicastrin Polyclonal Antibody (Product # PA5-20303) at (A) 0.5, (B) 1, and (C) 2 µg/mL.

Product Details

PA5-20303

Applications	Tested Dilution	Publications
Western Blot (WB)	0.5-2 µg/mL	-
Immunohistochemistry (IHC)	5 µg/mL	-

Product Specifications
Species Reactivity	Human, Mouse, Rat
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	A 17 amino acid peptide from near the carboxy terminus of human Nicastrin. View antigen
Conjugate	Unconjugated
Form	Liquid
Concentration	1 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS
Contains	0.02% sodium azide
Storage conditions	Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C
RRID	AB_11152451

Product Specific Information

A suggested positive control is mouse brain tissue lysate.

PA5-20303 can be used with blocking peptide PEP-0428.

Target Information

This gene encodes the largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. The product of this gene contains a carboxy terminal domain composed of heptapeptide repeats that are essential for polymerase activity. These repeats contain serine and threonine residues that are phosphorylated in actively transcribing RNA polymerase. In addition, this subunit, in combination with several other polymerase subunits, forms the DNA binding domain of the polymerase, a groove in which the DNA template is transcribed into RNA.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.