Invitrogen

Nkx2.2 Polyclonal Antibody

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Nkx2.2 Antibody in Western Blot (WB) — Western blot analysis of Nkx2.2 in non-transfected (-) and transfected (+) 293T cells using 30 µg of protein. Samples were separated with 12% SDS-PAGE and incubated with Nkx2.2 polyclonal antibody (Product # PA5-78079) using a dilution of 1:5000 followed by HRP-conjugated anti-rabbit IgG.

Product Details

Tested Applications

Dilution

Western Blot (WB)

1:5000

Product Specifications

Species Reactivity

Human, Mouse

Host / Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human Nkx2.2. The exact sequence is proprietary.

Conjugate

Unconjugated

Form

Liquid

Concentration

1 mg/ml

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7, with 20% glycerol

Contains

0.025% Proclin 300

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

RRID

AB_2736230

Product Specific Information

Positive Control: Nkx2.2-transfected 293T

Predicted Reactivity: Mouse (100%), Chicken (89%), Bovine (94%)

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial. For short-term storage (1-2 weeks), product can be stored at 4°C. For long-term storage, aliquot and store product at -20° C or below, avioiding multiple freeze-thaw cycles.

Target Information

Nkx2.2 encodes a protein that contains a homeobox domain and may be involved in the morphogenesis of the central nervous system. This gene is found on chromosome 20 near NKX2-4, and these two genes appear to be duplicated on chromosome 14 in the form of TITF1 and NKX2-8. The encoded protein is likely to be a nuclear transcription factor.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: Drosophila NK2 transcription factor related, locus 2; glycoprotein GP330, renal; Homeobox protein NK-2 homolog B; Homeobox protein Nkx-2.2; homeobox protein Nkx2-2; NK2 homeobox 2; NK2 transcription factor related, locus 2; NK2 transcription factor-like protein B; Nkx2-2; Nkx2.2; tinman

Gene Aliases: Nkx-2.2; NKX2-2; NKX2.2; NKX2B; tinman

UniProt ID: (Human) O95096, (Mouse) P42586

Entrez Gene ID: (Human) 4821, (Mouse) 18088

Molecular Function: DNA binding protein helix-turn-helix transcription factor homeobox transcription factor nucleic acid binding transcription factor