Invitrogen

Nkx2.2 Polyclonal Antibody

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Nkx2.2 Antibody in Immunocytochemistry (ICC/IF) — Immunocytochemistry-Immunofluorescence analysis of Nkx2.2 was performed in DIV9 rat E18 primary hippocampal neuron cells fixed in 4% paraformaldehyde at RT for 15 min. Green: Nkx2.2 Polyclonal Antibody (Product # PA5-78079) diluted at 1:500. Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody. Blue: Fluoroshield with DAPI.

Nkx2.2 Antibody in Immunohistochemistry (Frozen) (IHC (F)) — Immunohistochemistry (Frozen) analysis of Nkx2.2 was performed in frozen-sectioned rat E13.5 brain tissue using Nkx2.2 Polyclonal Antibody (Product # PA5-78079) at a dilution of 1:250 (Green). Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody diluted at 1:500. Blue: Fluoroshield with DAPI.

Nkx2.2 Antibody in Western Blot (WB) — Western Blot using Nkx2.2 Polyclonal Antibody (Product # PA5-78079). Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with Nkx2.2 Polyclonal Antibody (Product # PA5-78079) diluted at 1:5,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Product Details

PA5-78079

Applications	Tested Dilution	Publications
Western Blot (WB)	1:1,000-1:10,000	-
Immunohistochemistry (Frozen) (IHC (F))	1:100-1:1,000	-
Immunocytochemistry (ICC/IF)	1:100-1:1,000	-

Product Specifications
Species Reactivity	Human, Mouse, Rat
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Recombinant protein encompassing a sequence within the C-terminus region of human Nkx2.2. The exact sequence is proprietary. View antigen
Conjugate	Unconjugated
Form	Liquid
Concentration	1.13 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7, with 20% glycerol
Contains	0.025% Proclin 300
Storage conditions	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID	AB_2736230

Product Specific Information

Positive Control: Nkx2.2-transfected 293T

Predicted Reactivity: Mouse (100%), Chicken (89%), Bovine (94%)

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

The protein encoded by this gene contains a homeobox domain and may be involved in the morphogenesis of the central nervous system. This gene is found on chromosome 20 near NKX2-4, and these two genes appear to be duplicated on chromosome 14 in the form of TITF1 and NKX2-8. The encoded protein is likely to be a nuclear transcription factor.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Drosophila NK2 transcription factor related, locus 2; glycoprotein GP330, renal; Homeobox protein NK-2 homolog B; Homeobox protein Nkx-2.2; homeobox protein Nkx2-2; NK2 transcription factor related, locus 2; NK2 transcription factor-like protein B; Nkx2.2

Gene Aliases: Nkx-2.2; NKX2-2; NKX2.2; NKX2B; tinman

UniProt ID: (Human) O95096, (Mouse) P42586

Entrez Gene ID: (Human) 4821, (Rat) 366214, (Mouse) 18088

Molecular Function: homeodomain transcription factor