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Invitrogen
Nodal Polyclonal Antibody
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Product Details
PA5-28486
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein encompassing a sequence within the center region of human Nodal. The exact sequence is proprietary.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
0.1M tris glycine, pH 7, with 10% glycerol
Contains
0.01% thimerosal
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Recommended positive controls: Human ESC.
Predicted reactivity: Mouse (91%), Rat (92%), Bovine (93%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
NODAL is an important TGF-beta family protein in cellular differentiation. While constitutively expressed in many developing tissues, NODAL is problematically associated with the differentiation and development of many cancers. The expression of NODAL is regulated by a Notch signaling pathway, which when disrupted contributes to the aggressive behavior of metastatic cancer cells, particularly in melanoma. The abundance of NODAL expression in melanoma can help determine the stage of cancer and metastatic type/progression when tested in IHC. NODAL interactions with Cripto also predispose toward tumor progression. Overexpression of NODAL is also associated with preeclamptic placenta.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: early embryo mesoderm formation; MGC138230; Nodal; Nodal homolog; transgenic lethal 413.d
Gene Aliases: HTX5; NODAL; Tg.413d
UniProt ID: (Human) Q96S42, (Mouse) P43021
Entrez Gene ID: (Human) 4838, (Rat) 294503, (Mouse) 18119
negative regulation of transcription from RNA polymerase II promoter
trophectodermal cellular morphogenesis
neural fold formation
liver development
placenta development
embryonic placenta development
maternal placenta development
vasculature development
heart looping
inhibition of neuroepithelial cell differentiation
brain development
embryonic pattern specification
polarity specification of proximal/distal axis
regulation of gastrulation
positive regulation of vascular endothelial growth factor production
negative regulation of cell development
positive regulation of pathway-restricted SMAD protein phosphorylation
stem cell population maintenance
positive regulation of cell-cell adhesion
BMP signaling pathway
positive regulation of activin receptor signaling pathway
floor plate morphogenesis
endodermal cell differentiation
cell migration involved in gastrulation
positive regulation of cysteine-type endopeptidase activity involved in apoptotic process
positive regulation of angiogenesis
positive regulation of transcription from RNA polymerase II promoter
axial mesodermal cell fate specification
mesendoderm development
digestive tract morphogenesis
embryonic cranial skeleton morphogenesis
formation of anatomical boundary
positive regulation of epithelial cell proliferation
positive regulation of sequence-specific DNA binding transcription factor activity
embryonic process involved in female pregnancy
maternal process involved in parturition
positive regulation of SMAD protein import into nucleus
SMAD protein signal transduction
left lung morphogenesis
negative regulation of androgen receptor signaling pathway
epiblast cell-extraembryonic ectoderm cell signaling involved in anterior/posterior axis specification
positive regulation of ERK1 and ERK2 cascade
transforming growth factor beta receptor signaling pathway involved in primitive streak formation
nodal signaling pathway involved in determination of lateral mesoderm left/right asymmetry
positive regulation of nodal signaling pathway involved in determination of lateral mesoderm left/right asymmetry
negative regulation of trophoblast cell migration
negative regulation of chorionic trophoblast cell proliferation
regulation of stem cell population maintenance
in utero embryonic development
gastrulation with mouth forming second
mesoderm formation
trophectodermal cell differentiation
determination of left/right symmetry
gastrulation
nervous system development
endoderm development
heart development
positive regulation of cell proliferation
anterior/posterior axis specification
anterior/posterior pattern specification
regulation of signal transduction
cell migration
lung development
embryonic heart tube development
nodal signaling pathway
growth
regulation of apoptotic process
regulation of MAPK cascade
cell fate commitment
negative regulation of cell differentiation
cell development
anatomical structure formation involved in morphogenesis
tissue morphogenesis
digestive system development
primitive streak formation
positive regulation of receptor activity
transforming growth factor beta receptor signaling pathway
multicellular organism development
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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