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Invitrogen
Norepinephrine Transporter Polyclonal Antibody
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FIGURE: 1 / 3
Norepinephrine Transporter Antibody (PA5-89680) in WB
Western blot analysis of Norepinephrine Transporter in extracts of various cell lines. Samples were incubated with Norepinephrine Transporter Polyclonal antibody (Product # PA5-89680) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
Product Details
PA5-89680
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse,
Rat,
Human
Published species
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein (or fragment).
Conjugate
Form
Liquid
Concentration
2.99 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.05% ProClin 300
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Positive Samples: A-549, SH-SY5Y, Mouse kidney, Mouse spinal cord; Cellular Location: Membrane, Multi-pass membrane protein
Target Information
Norepinephrine Transporter [NET, or noradrenaline transporter (NAT)] is a monoamine transporter that transports the neurotransmitter noradrenaline from the synapse back to its vesicles for storage until later use. It also appears to transport the neurotransmitter dopamine in the same way, but to a lesser degree. Studies have shown a decrease in NET levels in the locus coeruleus in patients diagnosed with major depression. Cocaine, amphetamines and many therapeutic antidepressants, such as the SNRIs (serotonin-norepinephrine reuptake inhibitors) and the tricyclic antidepressants (TCAs) act to raise noradrenaline. Furthermore, deficits in the NET gene have been associated with ADHD.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: NaCl-dependent norepinephrine transporter; NE transporter; NET; neurotransmitter transporter; neurotransmitter transporter, noradrenal; noradrenaline; Norepinephrine transporter; Sodium-dependent noradrenaline transporter; solute carrier family 6 (neurotransmitter transporter), member 2; solute carrier family 6 (neurotransmitter transporter, noradrenalin), member 2; Solute carrier family 6 member 2; solute carrier family 6 member 5; transmembrane region 4..26 transmembrane region 55..77 transmembrane region 105..123 transmembrane region 136..157
Gene Aliases: NAT1; NE-T; NET; NET1; SLC6A2; SLC6A5
UniProt ID: (Human) P23975, (Mouse) O55192
Entrez Gene ID: (Human) 6530, (Rat) 83511, (Mouse) 20538
dopamine:sodium symporter activity
norepinephrine:sodium symporter activity
protein binding
monoamine transmembrane transporter activity
metal ion binding
neurotransmitter:sodium symporter activity
norepinephrine transmembrane transporter activity
alpha-tubulin binding
beta-tubulin binding
symporter activity
primary active transporter
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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