Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 30 min at room temperature. A suggested positive control is placenta or lung tissue.
Nfr1 (also designated TCF11), Nrf2 and Nfr3 are bZIP transcription factors that heterodimer with Maf proteins to bind MARE (Maf recognition elements) sequences. Nrf2 is widely expressed and is thought to translocate to the nucleus after treatment with xenobiotics and antioxidants, which stimulate its release from a repressor protein Keap1.
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Protein Aliases: E4TF1-60; E4TF1A; HEBP1; NF-E2-related factor 2; NFE2-related factor 2; NFT2; NRF2; NRF2A; nuclear factor erythroid 2-like 2; Nuclear factor erythroid 2-related factor 2; nuclear factor erythroid-derived 2-like 2; nuclear factor, erythroid 2-like 2; Nuclear factor, erythroid derived 2, like 2
Gene Aliases: AI194320; HEBP1; NFE2L2; Nrf-2; NRF2