|Tested species reactivity||Human|
|Published species reactivity||Human, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to C-terminus of human Nrf2 protein|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.6, with 1% BSA|
|Contains||<0.1% sodium azide|
|Storage Conditions||4° C, do not freeze|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 30 min at room temperature. A suggested positive control is placenta or lung tissue.
Nfr1 (also designated TCF11), Nrf2 and Nfr3 are bZIP transcription factors that heterodimer with Maf proteins to bind MARE (Maf recognition elements) sequences. Nrf2 is widely expressed and is thought to translocate to the nucleus after treatment with xenobiotics and antioxidants, which stimulate its release from a repressor protein Keap1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Transcription factor NFE2L2/NRF2 is a regulator of macroautophagy genes.
PA1-38312 was used in immunohistochemistry - paraffin section to identify NFE2L2/NRF2 as a regulator of autophagy gene expression and study its role in Alzheimer disease
|Pajares M,Jiménez-Moreno N,García-Yagüe ÁJ,Escoll M,de Ceballos ML,Van Leuven F,Rábano A,Yamamoto M,Rojo AI,Cuadrado A||Autophagy (12:1902)||2016|
Nrf2 deficiency impairs the barrier function of mouse oesophageal epithelium.
PA1-38312 was used in immunohistochemistry to study the role of Nrf2 in the maintenance of murine esophageal epithelial barrier function
|Chen H,Hu Y,Fang Y,Djukic Z,Yamamoto M,Shaheen NJ,Orlando RC,Chen X||Gut (63:711)||2014|
Transcript profiling identifies dynamic gene expression patterns and an important role for Nrf2/Keap1 pathway in the developing mouse esophagus.
PA1-38312 was used in immunohistochemistry to study the role of the Nrf2/Keap1 pathway in murine esophagus development
|Chen H,Li J,Li H,Hu Y,Tevebaugh W,Yamamoto M,Que J,Chen X||PloS one (7:null)||2012|