Invitrogen

Nrf2 Polyclonal Antibody

Product Image Gallery

Nrf2 Antibody — Fig. 5 Loss of GSH content in microglia influences oxidative stress but not cell viability. EOC13.31 microglia cells were divided into six groups: either untreated (control) or pretreated with 500 muM NAC or 200 muM BSO for 18 h, prior to 22 mM EtOH exposure for 6 h. Confocal images (20x) of cells labeled with CellROX Deep Red Reagent to detect ROS generation ( a ). Semi-quantification analysis of ROS fluorescence intensity ( b ). Intracellular GSH levels were determined fluorometrically by labeling cells with 50 muM Monochlorobimane ( c ). Western blot analysis for Nrf-2 expression and nuclear translocation, where Lamin B1 and beta actin was used as a loading control for nuclear and cytosolic fraction, respectively ( d ). Effect of the above treatment regimen on cell viability (MTS assay) ( e ). Values are presented as mean average +- SEM three independent experiments. Student-Newman-Keuls comparison analysis was used (* p <= 0.05; ** p <= 0.005)

Nrf2 Antibody in Immunofluorescence (IF) — Immunofluorescent analysis of NRF2 (green) HEK293T cells. Cells fixed with 4% formaldehyde were permeabilized and blocked with 1X PBS containing 5% BSA and 0.3% Triton X-100 for 1 hour at room temperature. Cells were probed with an NRF2 polyclonal antibody (Product # PA5-27882) at a dilution of 1:100 overnight at 4°C in 1X PBS containing 1% BSA and 0.3% Triton X-100, washed with 1X PBS, and incubated with a fluorophore-conjugated goat anti-rabbit IgG secondary antibody at a dilution of 1:200 for 1 hour at room temperature. Nuclei (blue) were stained with DAPI. Images were taken on a Leica DM1000 microscope at 40X magnification. Data courtesy of the Innovators Program.

Nrf2 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of NRF2 in paraffin-embedded Cal27 xenograft using a NRF2 polyclonal antibody (Product # PA5-27882) at a dilution of 1:500.

Nrf2 Antibody in Western Blot (WB) — Western blot analysis of NRF2 was performed by loading 20 µg of THP-1 whole cell lysate per well onto an SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat dry milk in TBST for 1 hour at room temperature. The membrane was probed with an NRF2 polyclonal antibody (Product # PA5-27882) at a dilution of 1:1000 overnight at 4°C, washed in TBST, and probed with an HRP-conjugated goat anti-rabbit IgG secondary antibody at a dilution of 1:40,000 for 1 hour at room temperature. Detection was performed using ECL substrate. Data courtesy of the Innovators Program.

Nrf2 Antibody in Immunoprecipitation (IP) — Immunoprecipitation of NRF2 in HepG2 whole cell lysates using 5 µg of NRF2 polyclonal antibody (Product # PA5-27882). Western blot analysis performed using NRF2 polyclonal antibody (Product # PA5-27882).

Nrf2 Antibody in ChIP assay (ChIP) — PA5-27882 antibody immunoprecipitates NRF2 protein-DNA in ChIP experiments. ChIP Sample: HeLa whole cell lysate/extract with 5 µg preimmune rabbit IgG (A) or 5 µg of NRF2 antibody (Product # PA5-27882) (B). The precipitated DNA was detected by PCR with primer set targeting to GCLC gene locus.

Product Details

Tested Applications

Dilution

ChIP assay (ChIP)

Assay-dependent

Immunocytochemistry (ICC)

1:100-1:1000

Immunofluorescence (IF)

1:100-1:1000

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1000

Immunoprecipitation (IP)

1:100-1:500

Western Blot (WB)

1:500-1:3000

Published Applications

Western Blot (WB)

See 2 publications below

Miscellaneous PubMed (MISC)

See 1 publication below

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human, Zebrafish

Host / Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fragment corresponding to a region within amino acids 108 and 413 of Human NRF2

Conjugate

Unconjugated

Form

Liquid

Concentration

0.11 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7, with 20% glycerol, 1% BSA

Contains

0.025% ProClin 300

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

RRID

AB_2545358

Product Specific Information

PA5-27882 targets NRF2 in IF, ChIP, IHC (P), and WB applications and shows reactivity with Human and mouse samples.

The PA5-27882 immunogen is recombinant fragment corresponding to a region within amino acids 108 and 413 of Human NRF2.

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial. For short-term storage (1-2 weeks), product can be stored at 4°C. For long-term storage, aliquot and store product at -20° C or below, avioiding multiple freeze-thaw cycles.

Target Information

NFE2 (MIM 601490), NFE2L1 (MIM 163260), and NFE2L2 comprise a family of human genes encoding basic leucine zipper (bZIP) transcription factors. They share highly conserved regions that are distinct from other bZIP families, such as JUN (MIM 165160) and FOS (MIM 164810), although remaining regions have diverged considerably from each other (Chan et al., 1995 [PubMed 7868116]).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: erythroid derived 2; HEBP1; like 2; NF-E2-related factor 2; NFE2-related factor 2; NRF2; nuclear factor; Nuclear factor erythroid 2-related factor 2; nuclear factor erythroid-derived 2-like 2; nuclear factor, erythroid 2-like 2; Nuclear factor, erythroid derived 2, like 2

Gene Aliases: AI194320; HEBP1; NFE2L2; Nrf-2; NRF2

UniProt ID: (Human) B4E338, (Mouse) Q60795

Entrez Gene ID: (Human) 4780, (Mouse) 18024

Molecular Function: actin family cytoskeletal protein cytoskeletal protein hydrolase non-motor actin binding protein nucleic acid binding protease serine protease transcription factor