Invitrogen
This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.
Applications | Tested Dilution | Publications |
---|---|---|
Western Blot (WB) |
1:500-1:3,000 | View 11 publications 11 publications |
Immunohistochemistry (IHC) |
- | View 2 publications 2 publications |
Immunohistochemistry (Paraffin) (IHC (P)) |
1:100-1:1,000 | - |
Immunocytochemistry (ICC/IF) |
1:100-1:1,000 | View 2 publications 2 publications |
Immunoprecipitation (IP) |
1:100-1:500 | View 1 publication 1 publication |
ChIP assay (ChIP) |
5 µg | - |
Miscellaneous PubMed (Misc) |
- | View 1 publication 1 publication |
Product Specifications | |
---|---|
Species Reactivity |
Avian, Human, Mouse, Plant, Reptile, Rat, Zebrafish |
Published species |
Human, Mouse, Pig, Zebrafish |
Host/Isotype |
Rabbit / IgG |
Class |
Polyclonal |
Type |
Antibody |
Immunogen |
Recombinant fragment corresponding to a region within amino acids 108 and 413 of Human NRF2 |
Conjugate |
Unconjugated |
Form |
Liquid |
Concentration |
0.41 mg/mL |
Purification |
Antigen affinity chromatography |
Storage buffer |
PBS, pH 7, with 20% glycerol, 1% BSA |
Contains |
0.025% ProClin 300 |
Storage conditions |
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
RRID |
AB_2545358 |
Recommended positive controls: C8D30, 3xFlag-human NFE2L2-transfected 293T, MDA-MB-231 nuclear extract, MDA-MB-231 nuclear extract (30 µM tBHQ, 4hr), HepG2 (10 µM MG132 treatment for 15 hr).
Predicted reactivity: Rhesus Monkey (99%), Bovine (84%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Nfr1 (also designated TCF11), Nrf2 and Nfr3 are bZIP transcription factors that heterodimer with Maf proteins to bind MARE (Maf recognition elements) sequences. Nrf2 is widely expressed and is thought to translocate to the nucleus after treatment with xenobiotics and antioxidants, which stimulate its release from a repressor protein Keap1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: E4TF1-60; E4TF1A; HEBP1; NF-E2-related factor 2; NFE2-related factor 2; NFT2; nrf2; NRF2A; nuclear factor erythroid 2-like 2; Nuclear factor erythroid 2-related factor 2; nuclear factor erythroid-derived 2-like 2; nuclear factor, erythroid 2-like 2; Nuclear factor, erythroid derived 2, like 2
Gene Aliases: AI194320; HEBP1; NFE2L2; NRF2
UniProt ID: (Human) Q16236, (Mouse) Q60795, (Rat) O54968
Entrez Gene ID: (Human) 4780, (Mouse) 18024, (Rat) 83619
Molecular Function:
basic leucine zipper transcription factor
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