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|Tested species reactivity||Human, Mouse, Primate, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Full-length recombinant human NuMA expressed in insect Sf9 cells using baculovirus expression system|
|Storage buffer||whole serum|
|Contains||0.1% sodium azide|
|Storage Conditions||-20°C or -80°C if preferred|
|Tested Applications||Dilution *|
|Immunohistochemistry (IHC)||5 µg/ml|
|Immunohistochemistry (Frozen) (IHC (F))||Assay-Dependent|
|Immunohistochemistry (Paraffin) (IHC (P))||5 µg/ml|
|Western Blot (WB)||1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Suggested positive control: Hela whole cell extract.
NuMA (nuclear mitotic apparatus) is a long coil-coiled protein that plays a role in the interphase and mitosis phases of a cell cycle. In interphase, it has been hypothesized to play a structural role in the nucleoskeleton that may be important in nuclear organization and functions. During mitosis, it is associated with spindle microtubule organization and chromosome positioning. Therefore, this antibody is useful as a cell cycle marker.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
centrophilin stabilizes mitotic spindle in mitotic cells; nuclear matrix protein-22; Nuclear Mitotic Apparatus Protein; SP-H antigen; structural nuclear protein
NMP-22; NMP22; NUMA; NUMA1