Invitrogen

Nuclear Matrix Protein p84 Polyclonal Antibody

Advanced Verification

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

View all (17) Nuclear Matrix Protein p84 antibodies

Datasheet

Protocols

Questions & Answers

Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

Product Image Gallery

Nuclear Matrix Protein p84 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescence analysis of Nuclear Matrix Protein p84 was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with Nuclear Matrix Protein p84 Polyclonal Antibody (Product # PA5-54056) at 5 µg/mL in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32790), (1:2000 dilution) for 45 minutes at room temperature (Panel a: Green). Nuclei (Panel b: Blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: Red) was stained with Rhodamine Phalloidin (Product # R415, 1:300 dilution). Panel d represents the merged image showing nuclear localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

Nuclear Matrix Protein p84 Antibody in Immunohistochemistry (IHC) — Immunohistochemical staining of Nuclear Matrix Protein p84 in human cerebral cortex, colon, skeletal muscle and testis using Nuclear Matrix Protein p84 Polyclonal Antibody (Product # PA5-54056) (A) shows similar protein distribution across tissues to an independent Nuclear Matrix Protein p84 Polyclonal Antibody (B).

Nuclear Matrix Protein p84 Antibody in Western Blot (WB) — Western blot was performed using anti-Nuclear Matrix Protein p84 Polyclonal Antibody (Product # PA5-54056) and a 75 kDa band corresponding to Nuclear Matrix Protein p84 was observed across all the cell lines and tissues tested. Whole cell extracts (30 µg lysate) of HeLa (Lane 1), A549 (Lane 2), MDA-MB-231 (Lane 3), MCF 10A (Lane 4), tissue extracts of Mouse Testis (Lane 5) and Mouse Skeletal Muscle (Lane 6) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Relative expression of Nuclear Matrix Protein p84 Polyclonal Antibody was observed high in Mouse testis and low or negative in Mouse skeletal Muscle. Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (0.25 µg/mL) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

Nuclear Matrix Protein p84 Antibody — Relative expression in different tissues in IHC: Detection of differential expression levels of Nuclear Matrix Protein p84 demonstrates antibody specificity. Immunohistochemical analysis of Nuclear Matrix Protein p84 using anti-Nuclear Matrix Protein p84 Polyclonal Antibody (Product # PA5-54056), shows significant staining of Nuclear Matrix Protein p84 in human testis and shows minimal or weak staining in human skeletal muscle tissues. The relative expression levels of Nuclear Matrix Protein p84 within each tissue is shown using RNA-Seq.

Product Details

PA5-54056

Applications

Tested Dilution

Publications

Western Blot (WB)

0.04-0.4 µg/mL

Immunohistochemistry (Paraffin) (IHC (P))

1:200-1:500

Immunocytochemistry (ICC/IF)

0.25-2 µg/mL

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein corresponding to Human Nuclear Matrix Protein p84

View immunogen

Conjugate

Unconjugated

Form

Liquid

Concentration

0.4 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.2, with 40% glycerol

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

RRID

AB_2644856

Product Specific Information

Immunogen sequence: KILMGNEELT RLWNLCPDNM EACKSETREH MPTLEEFFEE AIEQADPENM VENEYKAVNN SNYGWRALRL LARRSPHFFQ PTNQQFKSLP EYLENMVIKL AKELPPPSEE IKTGEDEDEE DNDALLK

Highest antigen sequence identity to the following orthologs: Mouse - 98%, Rat - 88%.

Target Information

HPR1 is part of the TREX (transcription/export) complex, which includes TEX1 (MIM 606929), THO2 (MIM 300395), ALY (MIM 604171), and UAP56 (MIM 142560).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.