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Invitrogen
Nucleolin Polyclonal Antibody
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FIGURE: 1 / 6
Nucleolin Antibody (PA5-19508) in ICC/IF
Immunofluorescence analysis of Nucleolin was performed using HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with Nucleolin Polyclonal Antibody (Product # PA5-19508) at 5 µg/mL in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with... View More
Product Details
PA5-19508
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human Nucleolin.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.4
Contains
0.02% sodium azide
Storage conditions
-20°C or -80°C if preferred
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Heat mediated antigen retrieval recommended prior to tissue staining.
Store antibody at 4°C for 1-2 weeks. For long-term storage, store at -20°C.
Target Information
Nucleolin (NCL), a eukaryotic nucleolar phosphoprotein, is involved in the synthesis and maturation of ribosomes. It is located mainly in dense fibrillar regions of the nucleolus. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. The intron 11 of the NCL gene encodes a small nucleolar RNA, termed U20.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: FLJ45706; FLJ59041; nucl antibody; Nucleolin; nucleolin-like protein; Protein C23
Gene Aliases: B530004O11Rik; C23; D0Nds28; D1Nds28; NCL; Nuc; Nucl
UniProt ID: (Human) P19338, (Mouse) P09405
Entrez Gene ID: (Human) 4691, (Mouse) 17975, (Rat) 25135
nucleotide binding
single-stranded DNA binding
RNA binding
calcium ion binding
protein binding
protein C-terminus binding
selenocysteine insertion sequence binding
rRNA primary transcript binding
telomeric DNA binding
histone binding
identical protein binding
laminin binding
poly(A) RNA binding
ErbB-4 class receptor binding
nucleic acid binding
DNA binding
receptor binding
sequence-specific DNA binding
angiogenesis
endocytosis
spermatogenesis
positive regulation of tumor necrosis factor production
negative regulation of apoptotic process
positive regulation of transcription from RNA polymerase II promoter
cellular response to lipopolysaccharide
liver regeneration
positive regulation of transcription of nuclear large rRNA transcript from RNA polymerase I promoter
regulation of rRNA processing
positive regulation of interleukin-6 secretion
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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