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Huabio
Nucleoside Phosphorylase Polyclonal Antibody
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FIGURE: 1 / 4
Nucleoside Phosphorylase Antibody (HA500062) in IHC (P)
Immunohistochemical analysis of paraffin-embedded human small intestine tissue using anti-Nucleoside Phosphorylase antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500062, 1:800) for 30 min... View More
Product Details
HA500062
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein within human Nucleoside Phosphorylase aa 1-200.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
TBS, pH 7.4, with 0.2% BSA, 50% glycerol
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
Target Information
The PNP gene encodes for the enzyme purine nucleoside phosphorylase, which plays a vital role in the purine salvage pathway by catalyzing the reversible cleavage of purine nucleosides. This enzyme facilitates the conversion of inosine, guanosine, and deoxyguanosine into free bases and sugars phosphorylated at the C1 position. PNP is located on chromosome 14q13 and is crucial for nucleoside catabolism, impacting energy metabolism and cellular functions across various organisms. PNP deficiency is an autosomal recessive multisystem disorder arising from mutations in the PNP gene, leading to impaired T-cell maturation and function, resulting in recurrent infections, autoimmune manifestations, and neurological abnormalities due to the toxic accumulation of deoxyguanosine triphosphate (dGTP). This deficiency particularly affects immature thymocytes and can lead to lymphoid and neuronal toxicity, underscoring the gene's importance in proper immune system function and cellular health.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: epididymis secretory sperm binding protein Li 156an; HEL-S-156an; Inosine phosphorylase; inosine phosphorylase {ECO:0000250|UniProtKB:P00491}; Inosine-guanosine phosphorylase; nucleoside phosphorylase; PNP; PNP {ECO:0000250|UniProtKB:P00491}; Purine nucleoside phosphorylase; purine nucleoside phosphorylase {ECO:0000250|UniProtKB:P00491}; purine-nucleoside:orthophosphate ribosyltransferase
Gene Aliases: NP; PNP; PRO1837; PUNP
UniProt ID: (Human) P00491
Entrez Gene ID: (Human) 4860, (Rat) 290029
nucleobase-containing compound metabolic process
inosine catabolic process
purine nucleotide catabolic process
nicotinamide riboside catabolic process
immune response
NAD biosynthesis via nicotinamide riboside salvage pathway
urate biosynthetic process
positive regulation of T cell proliferation
response to drug
purine-containing compound salvage
positive regulation of alpha-beta T cell differentiation
interleukin-2 secretion
nucleoside metabolic process
purine nucleoside metabolic process
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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