Proteintech

OCT4 Polyclonal Antibody

Product Image Gallery

OCT4 Antibody in Immunofluorescence (IF) — Confocal immunofluorescent analysis of human embronic stem cells with 11263-1-AP at dilution of 1:100. The PE shows staining with 11263-1-AP/PE. The DAPI shows nuclear staining by DAPI. The MERGE is the merge of PE and DAPI.

OCT4 Antibody in Western Blot (WB) — Human placenta tissue were subjected to SDS PAGE followed by western blot with 11263-1-AP (OCT4 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

Product Details

Tested Applications

Dilution

Western Blot (WB)

1:500-1:2000

Published Applications

Western Blot (WB)

See 49 publications below

Immunofluorescence (IF)

See 6 publications below

Immunohistochemistry (IHC)

See 11 publications below

Miscellaneous PubMed (MISC)

See 4 publications below

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse, Rat

Host / Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

OCT4 Fusion Protein Ag1794

Conjugate

Unconjugated

Form

Liquid

Concentration

0.36 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Target Information

Oct4 is a maternally expressed octamer-binding protein that was the first transcription factor described for the early stages of development. Oct4 is one of the four Yamaka factors, necessary for embryonic stem cell pluripotency. Pluripotent stem (iPS) cells, can be created by expressing Oct4 and the transcription factors Sox2, Klf4 and Lin28 along with c-Myc in mouse fibroblasts. More recently, experiments have demonstrated that iPS cells could be generated using expression plasmids expressing Oct4, Sox2, KlfF4 and c-Myc, eliminating the need for virus introduction, thereby addressing a safety concern for potential use of iPS cells in regenerative medicine. A translocation of this gene with the Ewing's sarcoma gene, t(6;22)(p21;q12), has been linked to tumor formation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: NF-A3; Oct 3; Oct 4; Oct-3; Oct-4; OCT3; OCT3/4; OCT4; Octamer binding protein 3; Octamer binding protein 4; Octamer-binding protein 3; Octamer-binding protein 4; Octamer-binding transcription factor 3; OTF 3; OTF-3; OTF3; OTF4; POU class 5 homeobox 1; POU class 5 homeobox 1 transcript variant OCT4B1; POU class 5 homeobox 1 transcript variant OCT4B2; POU class 5 homeobox 1 transcript variant OCT4B3; POU class 5 homeobox 1 transcript variant OCT4B4; POU class 5 homeobox 1 transcript variant OCT4B5; POU class 5 homeobox 1 transcript variant OCT4B6; POU domain class 5 transcription factor 1; POU domain transcription factor OCT4; POU domain, class 5, transcription factor 1; POU-type homeodomain-containing DNA-binding protein; POU5F1

Gene Aliases: NF-A3; Oct-3; Oct-3/4; Oct-4; OCT3; Oct3/4; OCT4; OTF-3; Otf-4; OTF3; Otf3-rs7; Otf3g; OTF4; POU5F1

UniProt ID: (Human) Q01860, (Mouse) Q63843

Entrez Gene ID: (Human) 5460, (Mouse) 18999, (Rat) 294562

Function(s)