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Proteintech

OCT4 Polyclonal Antibody

67 References
View all (57) OCT4 antibodies
Datasheet
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Datasheet
Protocols
Questions & Answers
Tech Support

Product Details

11263-1-AP

Applications Tested Dilution Publications

Western Blot (WB)

 1:500-1:2,000 View 49 publications 49 publications

Immunohistochemistry (IHC)

 - View 11 publications 11 publications

Immunocytochemistry (ICC/IF)

 - View 5 publications 5 publications

Miscellaneous PubMed (Misc)

 - View 2 publications 2 publications
Product Specifications

Species Reactivity

 Human, Mouse, Rat

Published species

 Human, Mouse, Rat

Host/Isotype

 Rabbit / IgG

Class

 Polyclonal

Type

 Antibody

Immunogen

OCT4 Fusion Protein Ag1794 (102-265 aa encoded by BC020712)
View immunogen

Conjugate

 Unconjugated

Form

 Liquid

Concentration

 0.36 mg/mL

Purification

 Antigen affinity chromatography

Storage buffer

 PBS, pH 7.3, with 50% glycerol

Contains

 0.02% sodium azide

Storage conditions

 -20°C

Product Specific Information

Immunogen sequence: MCKLRPLLQ KWVEEADNNE NLQEICKAET LVQARKRKRT SIENRVRGNL ENLFLQCPKP TLQQISHIAQ QLGLEKDVVR VWFCNRRQKG KRSSSDYAQR EDFEAAGSPF SGGPVSFPLA PGPHFGTPGY GSPHFTALYS SVPFPEGEAF PPVSVTTLGS PMHSN (102-265 aa encoded by BC020712)

Target Information

POU5F1, also commonly known as Oct-4, is a maternally expressed octamer-binding protein that was the first transcription factor described for the early stages of development. The role of POU5F1 in embryonic development suggested that it might be useful in the creation of stem cells that might be useful in cell replacement therapies in the treatment of several degenerative diseases. Artificial stem cells, termed induced pluripotent stem (iPS) cells, can be created by expressing POU5F1 and the transcription factors Sox2, Klf4 and Lin28 along with c-Myc in mouse fibroblasts. More recently, experiments have demonstrated that iPS cells could be generated using expression plasmids expressing POU5F1, Sox2, KlfF4 and c-Myc, eliminating the need for virus introduction, thereby addressing a safety concern for potential use of iPS cells in regenerative medicine.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: 3-Oct; 4-Oct; DADB-104B20.2; gp-9; HGNC:9221; MGC22487; NF-A3; Oct-3; Oct-4; Octamer-binding protein 3; Octamer-binding protein 4; Octamer-binding transcription factor 3; OTF-3; OTTHUMP00000221150; OTTHUMP00000221151; POU class 5 homeobox 1 transcript variant OCT4B1; POU class 5 homeobox 1 transcript variant OCT4B2; POU class 5 homeobox 1 transcript variant OCT4B3; POU class 5 homeobox 1 transcript variant OCT4B4; POU class 5 homeobox 1 transcript variant OCT4B5; POU class 5 homeobox 1 transcript variant OCT4B6; POU domain class 5 transcription factor 1; POU domain transcription factor OCT4; POU domain, class 5, transcription factor 1; pou-2; POU-type homeodomain-containing DNA-binding protein; pou2

Gene Aliases: NF-A3; Oct-3; Oct-3/4; Oct-4; OCT3; Oct3/4; OCT4; OTF-3; Otf-4; OTF3; Otf3-rs7; Otf3g; OTF4; POU5F1

UniProt ID: (Human) Q01860, (Mouse) P20263

Entrez Gene ID: (Human) 5460, (Mouse) 18999, (Rat) 294562

Function(s)
negative regulation of transcription from RNA polymerase II promoter mesodermal cell fate commitment endodermal cell fate commitment ectodermal cell fate commitment endodermal cell fate specification trophectodermal cell differentiation blastocyst growth BMP signaling pathway involved in heart induction transcription, DNA-templated regulation of transcription, DNA-templated transcription from RNA polymerase II promoter multicellular organism development response to organic substance regulation of gene expression stem cell population maintenance germ-line stem cell population maintenance response to retinoic acid somatic stem cell population maintenance positive regulation of catenin import into nucleus mRNA transcription from RNA polymerase II promoter cell fate commitment negative regulation of cell differentiation negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter negative regulation of calcium ion-dependent exocytosis stem cell differentiation positive regulation of protein kinase B signaling negative regulation of protein kinase B signaling positive regulation of SMAD protein import into nucleus cell fate commitment involved in formation of primary germ layer cardiac cell fate determination negative regulation of gene silencing by miRNA regulation of heart induction by regulation of canonical Wnt signaling pathway regulation of methylation-dependent chromatin silencing blastocyst development response to wounding anatomical structure morphogenesis regulation of asymmetric cell division response to cytokine
Process(es)
transcription regulatory region sequence-specific DNA binding RNA polymerase II transcription factor activity, sequence-specific DNA binding transcriptional repressor activity, RNA polymerase II transcription regulatory region sequence-specific binding DNA binding transcription factor activity, sequence-specific DNA binding protein binding ubiquitin protein ligase binding miRNA binding sequence-specific DNA binding poly(A) RNA binding RNA polymerase II core promoter proximal region sequence-specific DNA binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding RNA polymerase II transcription coactivator activity chromatin binding transcription corepressor activity transcription factor binding chromatin DNA binding transcription regulatory region DNA binding protein heterodimerization activity POU domain binding HMG box domain binding
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