|Tested species reactivity||Human, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||An 18 amino acid peptide near the carboxy terminus of human OGFOD1.|
|Purification||Antigen affinity chromatography|
|Contains||0.02% sodium azide|
|Storage Conditions||Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C|
|Tested Applications||Dilution *|
|Immunocytochemistry (ICC)||20 ug/ml|
|Immunofluorescence (IF)||20 ug/ml|
|Western Blot (WB)||1 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
A suggested positive control is Hela cell lysate.
PA5-21100 can be used with blocking peptide PEP-1214.
OGFOD1, a member of the 2-OG-Fe(II) dioxygenase family, plays an important role in ischemic survival and stress. It is an oxidoreductase containing a Prolyl 4-hydroxylase alpha subunit. An OGFOD1 iron binding residue is required for ATPAF1 gene expression. As a novel stress granule component, OGFOD1 plays important proapoptotic roles in the regulation of translation and HRI-mediated phosphorylation of eIF2alpha in cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.