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          • Primary Antibodies ›
          • beta Amyloid Antibodies

          Invitrogen

          Amyloid Precursor Protein Monoclonal Antibody (mAbP2-1)

          Advanced Verification
          4 Published Figures
          11 References
          View all (82) beta Amyloid antibodies

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          Cite Amyloid Precursor Protein Monoclonal Antibody (mAbP2-1)

          Additional Information:
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          • Antibody Testing Data (2)
          • Published Figures (4)
          • Advanced Verification (1)
          Amyloid Precursor Protein Antibody in Immunocytochemistry (ICC/IF)
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          Amyloid Precursor Protein Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          FIGURE: 1 / 7

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          Amyloid Precursor Protein Antibody (OMA1-03132) in ICC/IF

          Immunofluorescence analysis of Amyloid Precursor Protein was performed using 70% confluent log phase PC-3 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with Amyloid Precursor Protein Monoclonal Antibody (mAbP2-1) (Product # OMA1-03132) at 1:200 dilut... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          View Product

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          Amyloid Precursor Protein Antibody in Immunocytochemistry (ICC/IF)
          Amyloid Precursor Protein Antibody
          Amyloid Precursor Protein Antibody in Immunocytochemistry (ICC/IF)
          Amyloid Precursor Protein Antibody in Immunocytochemistry (ICC/IF)
          Amyloid Precursor Protein Antibody in Western Blot (WB)
          Amyloid Precursor Protein Antibody in Western Blot (WB)
          Amyloid Precursor Protein Antibody
          Amyloid Precursor Protein Monoclonal Antibody (mAbP2-1)

          Product Details

          OMA1-03132

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          5 µg/mL
          View 3 publications 3 publications

          Immunocytochemistry (ICC/IF)

          1:200
          View 1 publication 1 publication

          ELISA (ELISA)

          10 µg/mL
          View 6 publications 6 publications

          Immunoprecipitation (IP)

          25 µg/mL*
          View 1 publication 1 publication
          Product Specifications

          Species Reactivity

          Human, Non-human primate

          Published species

          Human, Mouse

          Host/Isotype

          Mouse / IgG1

          Class

          Monoclonal

          Type

          Antibody

          Clone

          mAbP2-1

          Immunogen

          Native, secreted form of APP
          3D Epitope / Immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          1.0 mg/mL

          Amount

          50 µg

          Purification

          Protein G

          Storage buffer

          PBS, pH 7.4

          Contains

          no preservative

          Storage conditions

          -80°C, Avoid Freeze/Thaw Cycles

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_325526

          Product Specific Information

          OMA1-03132 detects amyloid precursor protein (APP) from human and monkey tissues. This antibody is specific for native, non-denatured protein, and does not cross-react with mouse, rat APP or other APP homologs.

          OMA1-03132 has been successfully used in Western blot (non-reducing conditions), immunocytochemistry, immunoprecipitation and ELISA procedures. This antibody does not work in in IHC on paraffin embedded tissues nor does it recognize APP if the samples are boiled in Laemmli (SDS) sample buffer containing a reducing agent such as DTT or β-ME. Can be used in immunoprecipitation utilizing 25 µg/mL of OMA1-03132 linked sepharose.

          OMA1-03132 antigen is the native, secreted form of human APP. The epitope recognized by OMA1-03132 maps to residues 104-118 of APP

          Target Information

          Amyloid beta peptide (Abeta/Beta-amyloid) is the major constituent of amyloid plaques in the brains of individuals afflicted with Alzheimer's disease. Abeta peptide is 40-43 amino acids long and generated from the beta-amyloid precursor protein (beta APP) in a two-step process. The first step involves cleavage of the extracellular, amino-terminal domain of beta APP. Protein cleavage is performed by an aspartyl protease, beta-secretase (BACE) which is synthesized as a propeptide and must be modified to the mature and active form by the prohormone convertase, furin. Beta APP cleavage by the mature form of BACE results in the cellular secretion of a segment of beta APP, and a membrane-bound remnant. The remnant protein is processed by another protease, gamma-secretase. Gamma-secretase cleaves an intra-membrane site in the carboxyl-terminal domain of beta APP, thus generating the amyloid beta peptide. Gamma-secretase is believed to be a multi-subunit complex containing presenilin-1 and 2 as central components. The transmembrane glycoprotein, nicastrin, is associated with presinilins and has been found to bind to the carboxyl-terminus of beta APP and helps to modulate the production of the amyloid beta peptide. Abeta is an extracellular filamentous protein component of amyloid cores, neuritic plaques and is also found as a deposit in neurofibrillary tangles. Alzheimer's disease, the most common cause of senile dementia, is characterized by abnormal filamentous protein deposits in the brain. Beta amyloid deposits are also detected in Lewy body dementia, Down's syndrome, amyloidosis (Dutch type), cerebroarterial amyloidosis (cerebral amyloid angiopathy) and in the Guam Parkinson-Dementia complex.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          Bioinformatics

          Protein Aliases: ABPP; Alzheimer disease; Alzheimer disease amyloid A4 protein homolog; Alzheimer disease amyloid protein; Amyloid; Amyloid b; Amyloid beta; amyloid beta (A4) precursor protein; amyloid beta A4 protein; amyloid beta precursor protein (ABPP); amyloid beta protein; amyloid beta-protein precursor; Amyloid precursor protein; amyloid protein; Amyloid β; Amyloid-beta (A4) precursor protein; Amyloid-beta A4 protein; Amyloid-beta precursor protein; APP; APPI; beta-amyloid A4; beta-amyloid peptide; beta-amyloid peptide(1-40); beta-amyloid peptide(1-42); beta-amyloid precursor protein; Cerebral vascular amyloid peptide; CTF gamma; CTF-alpha; CVAP; OTTHUMP00000096096; Pan-Abeta; peptidase nexin-II; PN-II; PreA4; PreA4 751; Protease nexin-II; testicular tissue protein Li 2; unnamed protein product

          View more View less

          Gene Aliases: A4; AAA; ABETA; ABPP; AD1; alpha-sAPP; APP; APPI; CTFgamma; CVAP; PN-II; PN2; preA4

          View more View less

          UniProt ID: (Human) B2R5V1

          View more View less

          Entrez Gene ID: (Human) 351

          View more View less

          Function(s)
          G-protein coupled receptor binding DNA binding chromatin binding serine-type endopeptidase inhibitor activity receptor binding frizzled binding insulin receptor binding integrin binding protein binding heparin binding peptidase activator activity enzyme binding peptidase inhibitor activity receptor activator activity acetylcholine receptor activator activity acetylcholine receptor binding apolipoprotein binding chemoattractant activity identical protein binding protein homodimerization activity heparan sulfate proteoglycan binding metal ion binding ephrin receptor binding transition metal ion binding protein heterodimerization activity receptor agonist activity low-density lipoprotein particle receptor binding RAGE receptor binding chaperone binding PTB domain binding growth factor receptor binding amylin binding protein serine/threonine kinase binding molecular function activator activity heparan sulfate binding
          Process(es)
          negative regulation of transcription from RNA polymerase II promoter microglial cell activation response to yeast astrocyte activation involved in immune response regulation of transcription from RNA polymerase II promoter regulation of translation cellular copper ion homeostasis endocytosis apoptotic process cell adhesion G-protein coupled receptor signaling pathway adenylate cyclase-activating G-protein coupled receptor signaling pathway adenylate cyclase-inhibiting G-protein coupled receptor signaling pathway Notch signaling pathway chemical synaptic transmission axonogenesis central nervous system development learning or memory learning memory mating behavior locomotory behavior axo-dendritic transport negative regulation of cell proliferation associative learning adult locomotory behavior visual learning response to lead ion regulation of gene expression positive regulation of macromolecule metabolic process positive regulation of gene expression negative regulation of gene expression response to auditory stimulus microglia development axon midline choice point recognition neuron remodeling dendrite development calcium-mediated signaling antibacterial humoral response antifungal humoral response regulation of Wnt signaling pathway extracellular matrix organization neuron projection development phospholipase D-activating G-protein coupled receptor signaling pathway positive regulation of chemokine production positive regulation of interferon-gamma production positive regulation of interleukin-1 beta production positive regulation of interleukin-6 production positive regulation of tumor necrosis factor production positive regulation of superoxide anion generation regulation of toll-like receptor signaling pathway ionotropic glutamate receptor signaling pathway regulation of multicellular organism growth lipoprotein metabolic process positive regulation of apoptotic process positive regulation of MAPK cascade positive regulation of neuron apoptotic process innate immune response positive regulation of nitric oxide biosynthetic process positive regulation of neuron differentiation positive regulation of G-protein coupled receptor protein signaling pathway positive regulation of Toll signaling pathway positive regulation of glycolytic process positive regulation of mitotic cell cycle positive regulation of transcription from RNA polymerase II promoter positive regulation of JNK cascade astrocyte activation regulation of long-term neuronal synaptic plasticity collateral sprouting in absence of injury positive regulation of inflammatory response regulation of synapse structure or activity synapse organization defense response to Gram-negative bacterium defense response to Gram-positive bacterium positive regulation of calcium-mediated signaling positive regulation of cell activation cognition positive chemotaxis positive regulation of membrane protein ectodomain proteolysis regulation of protein metabolic process positive regulation of protein metabolic process protein homooligomerization protein tetramerization regulation of neurotransmitter uptake positive regulation of protein kinase B signaling low-density lipoprotein particle mediated signaling antimicrobial humoral immune response mediated by antimicrobial peptide protein trimerization positive regulation of ERK1 and ERK2 cascade response to interleukin-1 cellular response to copper ion cellular response to manganese ion cellular response to cAMP cellular response to norepinephrine stimulus positive regulation of monocyte chemotaxis negative regulation of canonical Wnt signaling pathway modulation of excitatory postsynaptic potential NMDA selective glutamate receptor signaling pathway positive regulation of gene expression, epigenetic positive regulation of cAMP/PKA signal transduction regulation of spontaneous synaptic transmission negative regulation of protein localization to nucleus regulation of beta-amyloid clearance negative regulation of long-term synaptic potentiation positive regulation of long-term synaptic potentiation positive regulation of NIK/NF-kappaB signaling regulation of calcium ion transmembrane transport via high voltage-gated calcium channel negative regulation of pri-miRNA transcription from RNA polymerase II promoter regulation of dendritic spine maintenance positive regulation of amyloid precursor protein catabolic process regulation of endoplasmic reticulum stress-induced neuron intrinsic apoptotic signaling pathway negative regulation of blood circulation positive regulation of G protein-coupled receptor internalization positive regulation of endothelin production positive regulation of protein import cellular response to beta-amyloid regulation of presynapse assembly positive regulation of response to endoplasmic reticulum stress regulation of amyloid fibril formation positive regulation of amyloid fibril formation regulation of response to calcium ion amyloid fibril formation cellular response to nerve growth factor stimulus neuron projection maintenance positive regulation of T cell migration positive regulation of excitatory postsynaptic potential positive regulation of extrinsic apoptotic signaling pathway
          It has to be done as per old AB suggested Products section.

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