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OPN-R Antibody (711215) in ICC/IF
Immunofluorescence was performed on fixed and permeabilized U-2 OS cells for detection of endogenous cleaved Osteopontin using Anti- Osteopontin (cleaved) Recombinant Rabbit Superclonal™ Antibody (Product # 711215, 2 µg/mL) and labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2000). Panel a) shows representative cells that were stained for detection and localization of cleaved Osteopontin protein (green), Panel b) is stained for nuclei (blue) using SlowFade® Go... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
711215
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Mouse
Host/Isotype
Rabbit
/ IgG
Expression System
Expi293
Class
Recombinant Superclonal
Type
Antibody
Clone
24 HCLC
Immunogen
Peptide corresponding to Human SPP1 (aa 158-165)
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
0.5 mg/mL
Amount
100 µg
Purification
Protein A
Storage buffer
PBS, pH 7.2-7.4
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
Product Specific Information
This antibody is predicted to react with Monkey, Rabbit and Rat.
Recombinant rabbit Superclonal™ antibodies are unique offerings from Thermo Fisher Scientific. They are comprised of a selection of multiple different recombinant monoclonal antibodies, providing the best of both worlds - the sensitivity of polyclonal antibodies with the specificity of monoclonal antibodies - all delivered with the consistency only found in a recombinant antibody. While functionally the same as a polyclonal antibody - recognizing multiple epitope sites on the target and producing higher detection sensitivity for low abundance targets - a recombinant rabbit Superclonal™ antibody has a known mixture of light and heavy chains. The exact population can be produced in every lot, circumventing the biological variability typically associated with polyclonal antibody production. Note: Formerly called “Recombinant polyclonal antibody”, this product is now rebranded as “Recombinant Superclonal™ antibody”. The physical product and the performance remain unchanged.
Target Information
Osteopontin is a 34kDa extracellular matrix protein with a cell binding domain. It was originally identified as a major component of the non-collagenous organic bone matrix, however, it has subsequently been demonstrated in a wide range of normal adult tissue and body fluids. It is a multifunctional protein involved in bone mineralization, cell adhesion, cell migration, chronic inflammatory disease and transformation. Proteolytic cleavage by thrombin and matrix metalloproteinases close to the integrin-binding Arg-Gly-Asp sequence modulates the function of OPN and its integrin binding properties. Thrombin-cleaved fragments of Osteopontin are overexpressed in malignant glial tumors and provide a molecular niche with survival advantage and provide a novel substrate for plasmin and cathepsin D.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: 44 kDa bone phosphoprotein; bone sialoprotein 1; calcium oxalate crystal growth inhibitor protein; early T-lymphocyte activation 1; early T-lymphocyte activation 1 protein; lnc-PKD2-2-3; minopontin; nephropontin; OSN-C; OSN-D; osteopontin precursor; osteopontin-like; osteopontin-like protein; osteopontin/immunoglobulin alpha 1 heavy chain constant region fusion protein; putative OPN isoform 6; secreted phosphoprotein 1 (osteopontin, bone sialoprotein I, early T-lymphocyte activation 1); Sialoprotein (osteopontin); SPP-1; Spp1; SPP1/CALPHA1 fusion; Thrombin OPN-R; unnamed protein product; urinary stone protein; uropontin
Gene Aliases: 2AR; Apl-1; BNSP; Bsp; BSPI; Eta; ETA-1; OP; OPN; Opnl; OSP; Ric; Spp-1
UniProt ID: (Human) P10451, (Mouse) P10923, (Rat) P08721
Entrez Gene ID: (Human) 6696, (Mouse) 20750, (Rat) 25353
ossification
osteoblast differentiation
androgen catabolic process
cell adhesion
embryo implantation
cell differentiation
bone mineralization
response to vitamin D
response to macrophage colony-stimulating factor
positive regulation of bone resorption
positive regulation of transcription, DNA-templated
decidualization
response to steroid hormone
negative regulation of collateral sprouting of intact axon in response to injury
calcium ion homeostasis
cellular response to testosterone stimulus
response to 2,3,7,8-tetrachlorodibenzodioxine
positive regulation of estradiol secretion
cellular calcium ion homeostasis
cellular sodium ion homeostasis
signal transduction
positive regulation of cell-substrate adhesion
neutrophil chemotaxis
cellular phosphate ion homeostasis
cellular chloride ion homeostasis
urate biosynthetic process
negative regulation of apoptotic process
cellular response to fluid shear stress
collecting duct development
cellular response to leukemia inhibitory factor
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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