PA1-24628 detects mu Opioid Receptor from human, mouse, rat, and non-human primate samples. PA1-24628 is expected to cross react with bovine (93% conserved), guinea pig (100% conserved), Macaque monkey (100% conserved), and porcine (100% conserved) due to sequence homology.
PA1-24628 has been successfully used in immunohistochemistry (frozen), immunocytochemistry, immunofluorescence and Western blot procedures. The molecular weight of the protein detected by PA1-24628 is 65 kDa.
The PA1-24628 immunogen is a synthetic peptide corresponding to the C-terminal residues N(384) H Q L E N L E A E T A P L P(398) of rat mu Opioid Receptor.
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial. For short-term storage (1-2 weeks), product can be stored at 4°C. For long-term storage, aliquot and store product at -20° C or below, avioiding multiple freeze-thaw cycles.
The opioid receptors are G-protein coupled, seven-transmembrane domain receptors for enkephalins, endorphins, and dynorphins. Three different opioid receptor subtypes (kappa , delta, and mu) were first identified by their different selectivities for various naturally occurring alkaloid opioid ligands, and subsequently confirmed by molecular cloning. The amino acid sequences of the opioid receptor subtypes are ~70% homologous, and are similar to somatostatin receptors (SSTRs) showing ~40 % homology with SSTR1. G-protein binding is thought to occur at the third intracellular loop of the opioid receptors, which is also the location of consensus sequences for phosphorylation of the receptor. Interestingly, the genes encoding the specific receptor subtypes are found on different chromosomes in both the human and mouse genomes.
The mu opioid receptor is widely distributed throughout the central nervous system, with higher levels found in the periaqueductal gray region and in the superficial dorsal horn of the spinal cord. The mu receptor demonstrates high affinity to enkephalins and beta-endorphin, and low affinity to dynorphins. The mu opioid receptor is also the high affinity binding protein for the opiate alkaloids such as morphine, codeine, and methadone.
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Protein Aliases: M-OR-1; MOP; MOP receptor; MOR-1; Mu opiate receptor; Mu opioid receptor; mu opioid receptor hMOR-1a; mu opioid receptor splice variant mMOR-1Z; mu opioid receptor splice variant rMOR-1S; mu opioid receptor splice variant rMOR-1Z; mu-opioid receptor; Mu-type opioid receptor; MUOR1; Opioid receptor B; opioid receptor, mu 1; OPRM1
Gene Aliases: BOS_9964; LMOR; M-OR-1; MOP; MOP-R; MOR; MOR-1; MOR-1O; MOR1; MORA; muOR; OPRM; OPRM1; Oprrm1; Ror-b