|Tested species reactivity||Human, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide made to an internal portion of isoform 1 of the human OS9 protein (within residues 300-400).|
|Purification||Antigen affinity chromatography|
|Storage buffer||tris citrate, pH 7-8|
|Contains||0.01% sodium azide|
|Storage Conditions||4° C, do not freeze|
|Tested Applications||Dilution *|
|Immunocytochemistry (ICC)||0.2-1 µg/ml|
|Immunofluorescence (IF)||0.2-1 µg/ml|
|Immunohistochemistry (IHC)||0.5-1.0 µg/ml|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100-1:250|
|Western Blot (WB)||0.2-1 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
In Western blot, bands can be seen at ~75 kDa representing isoform 1 and at ~70 kDa representing isoform 2.
Suggested positive control: HEK293 postnuclear extracts, antigen standard for OS9 (transient overexpression lysate).
OS9 is an ER glycoprotein found in the intralumenal level and highly expressed in tumor tissue. The OS9 gene is transcriptionally induced upon activation of the Ire1/Xbp1 ER-stress pathway.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.