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          • Primary Antibodies ›
          • OTUB1/2 Antibodies

          Bioss

          OTUB Polyclonal Antibody

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          Cite OTUB Polyclonal Antibody

          Additional Information:
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          • Antibody Testing Data (1)
          OTUB Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Group 53 Created with Sketch.
          OTUB Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Group 53 Created with Sketch.

          FIGURE: 1 / 1

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          OTUB Antibody (BS-6236R) in IHC (P)

          Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with OTUB Polyclonal Antibody, Unconjugated (bs-6236R) at 1:200 overnight at 4°C, DAB staining. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          OTUB Antibody in Immunohistochemistry (Paraffin) (IHC (P))

          Product Details

          BS-6236R

          Applications
          Tested Dilution
          Publications

          Immunohistochemistry (Paraffin) (IHC (P))

          1:200
          -

          Immunohistochemistry (Frozen) (IHC (F))

          1:100-1:500
          -

          Immunocytochemistry (ICC/IF)

          1:50-1:200
          -

          ELISA (ELISA)

          1:500-1:1,000
          -
          Product Specifications

          Species Reactivity

          Human, Mouse, Rat

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          KLH conjugated synthetic peptide derived from human OTUB.

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          1 mg/mL

          Amount

          100 µg

          Purification

          Protein A

          Storage buffer

          0.01M TBS, pH 7.4, with 50% glycerol, 1% BSA

          Contains

          0.02% ProClin 300

          Storage conditions

          -20°C

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          Target Information

          This gene encodes one of several deubiquitylating enzymes. Ubiquitin modification of proteins is needed for their stability and function; to reverse the process, deubiquityling enzymes remove ubiquitin. This protein contains an OTU domain and binds Ubal (ubiquitin aldehyde); an active cysteine protease site is present in the OTU domain.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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          Cite this product

          Bioinformatics

          Protein Aliases: C14orf137; Deubiquitinating enzyme OTUB1; deubiquitinating enzyme OTUB1 {ECO:0000250|UniProtKB:Q7TQI3}; Deubiquitinating enzyme OTUB2; hOTU1; OTU domain, ubiquitin aldehyde binding 1; OTU domain, ubiquitin aldehyde binding 2; OTU domain-containing ubiquitin aldehyde-binding protein 1; OTU domain-containing ubiquitin aldehyde-binding protein 1 {ECO:0000250|UniProtKB:Q7TQI3}; OTU domain-containing ubiquitin aldehyde-binding protein 2; OTU-domain Ubal-binding 1; otub1 {ECO:0000312|EMBL:AAI66771.1}; otubain 1; Otubain-1; otubain-1 {ECO:0000250|UniProtKB:Q7TQI3}; Otubain-2; Ubiquitin thioesterase OTUB1; ubiquitin thioesterase OTUB1 {ECO:0000250|UniProtKB:Q7TQI3}; Ubiquitin thioesterase OTUB2; ubiquitin-specific protease otubain 1; ubiquitin-specific protease otubain 2; Ubiquitin-specific-processing protease OTUB1; ubiquitin-specific-processing protease OTUB1 {ECO:0000250|UniProtKB:Q7TQI3}; Ubiquitin-specific-processing protease OTUB2; unnamed protein product

          View more View less

          Gene Aliases: 2010015L18Rik; 4930586I02Rik; AI413508; AI850305; AW557219; C14orf137; HSPC263; OTB1; OTB2; OTU1; OTU2; OTUB1; OTUB2

          View more View less

          UniProt ID: (Human) Q96FW1, (Human) Q96DC9, (Mouse) Q7TQI3, (Rat) B2RYG6, (Mouse) Q9CQX0

          View more View less

          Entrez Gene ID: (Human) 55611, (Human) 78990, (Mouse) 107260, (Rat) 293705, (Mouse) 68149, (Rat) 314405

          View more View less

          Function(s)
          thiol-dependent ubiquitin-specific protease activity protein binding peptidase activity cysteine-type peptidase activity hydrolase activity NEDD8-specific protease activity ubiquitin protein ligase binding ubiquitin binding ubiquitin-protein transferase inhibitor activity molecular_function cysteine protease
          Process(es)
          adaptive immune response immune system process DNA repair proteolysis cellular response to DNA damage stimulus protein deubiquitination cellular response to nutrient levels TORC1 signaling proteasome-mediated ubiquitin-dependent protein catabolic process protein K48-linked deubiquitination negative regulation of TORC1 signaling positive regulation of TORC1 signaling negative regulation of double-strand break repair protein K11-linked deubiquitination protein K63-linked deubiquitination cellular response to interleukin-1 negative regulation of histone H2A K63-linked ubiquitination cellular amino acid metabolic process
          It has to be done as per old AB suggested Products section.

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