Proteintech

OTUD7B Polyclonal Antibody

OTUD7B Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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OTUD7B Antibody (16605-1-AP) in IHC (P)

Immunohistochemistry of paraffin-embedded mouse kidney using 16605-1-AP (OTUD7B antibody) at dilution of 1:50 (under 40x lens).

OTUD7B Antibody in Immunoprecipitation (IP)

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Product Details

16605-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

View 10 publications 10 publications

Immunohistochemistry (IHC)

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:500

Immunocytochemistry (ICC/IF)

View 6 publications 6 publications

Immunoprecipitation (IP)

0.5-4.0 µg

View 2 publications 2 publications

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

OTUD7B Fusion Protein Ag9788 (1-355 aa encoded by BC020622)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.22 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: MTLDMDAVL SDFVRSTGAE PGLARDLLEG KNWDVNAALS DFEQLRQVHA GNLPPSFSEG SGGSRTPEKG FSDREPTRPP RPILQRQDDI VQEKRLSRGI SHASSSIVSL ARSHVSSNGG GGGSNEHPLE MPICAFQLPD LTVYNEDFRS FIERDLIEQS MLVALEQAGR LNWWVSVDPT SQRLLPLATT GDGNCLLHAA SLGMWGFHDR DLMLRKALYA LMEKGVEKEA LKRRWRWQQT QQNEESGLVY TEDEWQKEWN ELIKLASSEP RMHLGTNGAN CGGVESSEEP VYESLEEFHV FVLAHVLRRP IVVVADTMLR DSGGEAFAPI PFGGIYLPLE VPASQCHRSP LVLAYD (1-355 aa encoded by B C020622)

Target Information

Negative regulator of the non-canonical NF-kappa-B pathway that acts by mediating deubiquitination of TRAF3, an inhibitor of the NF-kappa-B pathway, thereby acting as a negative regulator of B-cell responses. In response to non-canonical NF-kappa-B stimuli, deubiquitinates 'Lys-48'-linked polyubiquitin chains of TRAF3, preventing TRAF3 proteolysis and over-activation of non-canonical NF-kappa-B. Negatively regulates mucosal immunity against infections. Deubiquitinates ZAP70, and thereby regulates T cell receptor (TCR) signaling that leads to the activation of NF-kappa-B (PubMed:26903241). Plays a role in T cell homeostasis and is required for normal T cell responses, including production of IFNG and IL2. Mediates deubiquitination of EGFR (PubMed:22179831). Has deubiquitinating activity toward 'Lys-11', 'Lys-48' and 'Lys-63'-linked polyubiquitin chains (PubMed:27732584). Has a much higher catalytic rate with 'Lys-11'-linked polyubiquitin chains (in vitro); however the physiological significance of these data are unsure (PubMed:27732584). Hydrolyzes both linear and branched forms of polyubiquitin. [UniProt]

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

AtomNet-Aided OTUD7B Inhibitor Discovery and Validation.

Cancers

Chen J,Bolhuis DL,Laggner C,Kong D,Yu L,Wang X,Emanuele MJ,Brown NG,Liu P

Sat Jan 14 00:00:00 UTC 2023

Differential activity of transcription factor Sox9 in early and adult oligodendroglial progenitor cells.

Glia

Hassel LA,Fröb F,Küspert M,Hillgärtner S,Arnold P,Huang W,Kirchhoff F,Wegner M

Tue Aug 01 00:00:00 UTC 2023

OTUD7B Deubiquitinates LSD1 to Govern Its Binding Partner Specificity, Homeostasis, and Breast Cancer Metastasis.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)

Gong Z,Li A,Ding J,Li Q,Zhang L,Li Y,Meng Z,Chen F,Huang J,Zhou D,Hu R,Ye J,Liu W,You H

Sun Aug 01 00:00:00 UTC 2021

Transcranial direct-current stimulation protects against cerebral ischemia-reperfusion injury through regulating Cezanne-dependent signaling.

Experimental neurology

Cheng J,Fan YQ,Jiang HX,Chen SF,Chen J,Liao XY,Zou YY,Lan HY,Cui Y,Chen ZB,Chen QX,Wan Q

Mon Nov 01 00:00:00 UTC 2021

The Clock-Controlled lncRNA-AK028245 Participates in the Immune Response via Immune Response Factors OTUD7B and A20.

Journal of biological rhythms

Qi F,Jiang Z,Hou W,Peng B,Cheng S,Zhang X,Luo Z,Dai Z,Wang Y,Liu Y,Wang Y,Wang Z

Tue Dec 01 00:00:00 UTC 2020

Cezanne contributes to cancer progression by playing a key role in the deubiquitination of IGF-1R.

American journal of cancer research

Chen X,Pang Z,Wang Y,Zhu L,Liu J,Du J

Mon Jan 11 00:00:00 UTC 2021

Salvia miltiorrhiza-derived miRNAs suppress vascular remodeling through regulating OTUD7B/KLF4/NMHC IIA axis.

Theranostics

Yang GS,Zheng B,Qin Y,Zhou J,Yang Z,Zhang XH,Zhao HY,Yang HJ,Wen JK

Wed May 05 00:00:00 UTC 2021

Salvia miltiorrhiza-derived miRNAs suppress vascular remodeling through regulating OTUD7B/KLF4/NMHC IIA axis.

Theranostics

Yang GS,Zheng B,Qin Y,Zhou J,Yang Z,Zhang XH,Zhao HY,Yang HJ,Wen JK

Wed May 05 00:00:00 UTC 2021

Salvia miltiorrhiza-derived miRNAs suppress vascular remodeling through regulating OTUD7B/KLF4/NMHC IIA axis.

Theranostics

Yang GS,Zheng B,Qin Y,Zhou J,Yang Z,Zhang XH,Zhao HY,Yang HJ,Wen JK

Wed May 05 00:00:00 UTC 2021

Salvia miltiorrhiza-derived miRNAs suppress vascular remodeling through regulating OTUD7B/KLF4/NMHC IIA axis.

Theranostics

Yang GS,Zheng B,Qin Y,Zhou J,Yang Z,Zhang XH,Zhao HY,Yang HJ,Wen JK

Wed May 05 00:00:00 UTC 2021

Upregulation of OTUD7B (Cezanne) Promotes Tumor Progression via AKT/VEGF Pathway in Lung Squamous Carcinoma and Adenocarcinoma.

Frontiers in oncology

Lin DD,Shen Y,Qiao S,Liu WW,Zheng L,Wang YN,Cui N,Wang YF,Zhao S,Shi JH

Thu Oct 01 00:00:00 UTC 2020

The N-terminal ubiquitin-associated domain of Cezanne is crucial for its function to suppress NF-κB pathway.

Journal of cellular biochemistry

Ji Y,Cao L,Zeng L,Zhang Z,Xiao Q,Guan P,Chen S,Chen Y,Wang M,Guo D

Thu Feb 01 00:00:00 UTC 2018

The N-terminal ubiquitin-associated domain of Cezanne is crucial for its function to suppress NF-κB pathway.

Journal of cellular biochemistry

Ji Y,Cao L,Zeng L,Zhang Z,Xiao Q,Guan P,Chen S,Chen Y,Wang M,Guo D

Thu Feb 01 00:00:00 UTC 2018

The N-terminal ubiquitin-associated domain of Cezanne is crucial for its function to suppress NF-κB pathway.

Journal of cellular biochemistry

Ji Y,Cao L,Zeng L,Zhang Z,Xiao Q,Guan P,Chen S,Chen Y,Wang M,Guo D

Thu Feb 01 00:00:00 UTC 2018

The N-terminal ubiquitin-associated domain of Cezanne is crucial for its function to suppress NF-κB pathway.

Journal of cellular biochemistry

Ji Y,Cao L,Zeng L,Zhang Z,Xiao Q,Guan P,Chen S,Chen Y,Wang M,Guo D

Thu Feb 01 00:00:00 UTC 2018

Dynamic ubiquitylation of Sox2 regulates proteostasis and governs neural progenitor cell differentiation.

Nature communications

Cui CP,Zhang Y,Wang C,Yuan F,Li H,Yao Y,Chen Y,Li C,Wei W,Liu CH,He F,Liu Y,Zhang L

Wed Nov 07 00:00:00 UTC 2018

Dynamic ubiquitylation of Sox2 regulates proteostasis and governs neural progenitor cell differentiation.

Nature communications

Cui CP,Zhang Y,Wang C,Yuan F,Li H,Yao Y,Chen Y,Li C,Wei W,Liu CH,He F,Liu Y,Zhang L

Wed Nov 07 00:00:00 UTC 2018

Mechanical Activation of Hypoxia-Inducible Factor 1α Drives Endothelial Dysfunction at Atheroprone Sites.

Arteriosclerosis, thrombosis, and vascular biology

Feng S,Bowden N,Fragiadaki M,Souilhol C,Hsiao S,Mahmoud M,Allen S,Pirri D,Ayllon BT,Akhtar S,Thompson AAR,Jo H,Weber C,Ridger V,Schober A,Evans PC

Wed Nov 01 00:00:00 UTC 2017

Mechanical Activation of Hypoxia-Inducible Factor 1α Drives Endothelial Dysfunction at Atheroprone Sites.

Arteriosclerosis, thrombosis, and vascular biology

Feng S,Bowden N,Fragiadaki M,Souilhol C,Hsiao S,Mahmoud M,Allen S,Pirri D,Ayllon BT,Akhtar S,Thompson AAR,Jo H,Weber C,Ridger V,Schober A,Evans PC

Wed Nov 01 00:00:00 UTC 2017

Mechanical Activation of Hypoxia-Inducible Factor 1α Drives Endothelial Dysfunction at Atheroprone Sites.

Arteriosclerosis, thrombosis, and vascular biology

Feng S,Bowden N,Fragiadaki M,Souilhol C,Hsiao S,Mahmoud M,Allen S,Pirri D,Ayllon BT,Akhtar S,Thompson AAR,Jo H,Weber C,Ridger V,Schober A,Evans PC

Wed Nov 01 00:00:00 UTC 2017

Mechanical Activation of Hypoxia-Inducible Factor 1α Drives Endothelial Dysfunction at Atheroprone Sites.

Arteriosclerosis, thrombosis, and vascular biology

Feng S,Bowden N,Fragiadaki M,Souilhol C,Hsiao S,Mahmoud M,Allen S,Pirri D,Ayllon BT,Akhtar S,Thompson AAR,Jo H,Weber C,Ridger V,Schober A,Evans PC

Wed Nov 01 00:00:00 UTC 2017

Otud7b facilitates T cell activation and inflammatory responses by regulating Zap70 ubiquitination.

The Journal of experimental medicine

Hu H,Wang H,Xiao Y,Jin J,Chang JH,Zou Q,Xie X,Cheng X,Sun SC

Mon Mar 07 00:00:00 UTC 2016

Otud7b facilitates T cell activation and inflammatory responses by regulating Zap70 ubiquitination.

The Journal of experimental medicine

Hu H,Wang H,Xiao Y,Jin J,Chang JH,Zou Q,Xie X,Cheng X,Sun SC

Mon Mar 07 00:00:00 UTC 2016

OTUD7B controls non-canonical NF-κB activation through deubiquitination of TRAF3.

Nature

Hu H,Brittain GC,Chang JH,Puebla-Osorio N,Jin J,Zal A,Xiao Y,Cheng X,Chang M,Fu YX,Zal T,Zhu C,Sun SC

Thu Feb 21 00:00:00 UTC 2013

OTUD7B controls non-canonical NF-κB activation through deubiquitination of TRAF3.

Nature

Hu H,Brittain GC,Chang JH,Puebla-Osorio N,Jin J,Zal A,Xiao Y,Cheng X,Chang M,Fu YX,Zal T,Zhu C,Sun SC

Thu Feb 21 00:00:00 UTC 2013

OTUD7B controls non-canonical NF-κB activation through deubiquitination of TRAF3.

Nature

Hu H,Brittain GC,Chang JH,Puebla-Osorio N,Jin J,Zal A,Xiao Y,Cheng X,Chang M,Fu YX,Zal T,Zhu C,Sun SC

Thu Feb 21 00:00:00 UTC 2013

OTUD7B controls non-canonical NF-κB activation through deubiquitination of TRAF3.

Nature

Hu H,Brittain GC,Chang JH,Puebla-Osorio N,Jin J,Zal A,Xiao Y,Cheng X,Chang M,Fu YX,Zal T,Zhu C,Sun SC

Thu Feb 21 00:00:00 UTC 2013

Cezanne regulates inflammatory responses to hypoxia in endothelial cells by targeting TRAF6 for deubiquitination.

Circulation research

Luong le A,Fragiadaki M,Smith J,Boyle J,Lutz J,Dean JL,Harten S,Ashcroft M,Walmsley SR,Haskard DO,Maxwell PH,Walczak H,Pusey C,Evans PC

Fri Jun 07 00:00:00 UTC 2013

Cezanne regulates inflammatory responses to hypoxia in endothelial cells by targeting TRAF6 for deubiquitination.

Circulation research

Luong le A,Fragiadaki M,Smith J,Boyle J,Lutz J,Dean JL,Harten S,Ashcroft M,Walmsley SR,Haskard DO,Maxwell PH,Walczak H,Pusey C,Evans PC

Fri Jun 07 00:00:00 UTC 2013

Bioinformatics

Protein Aliases: Cellular zinc finger anti-NF-kappa-B protein; cellular zinc finger anti-NF-kappaB Cezanne; Cezanne; OTU domain containing 7B; OTU domain-containing protein 7B; TRAF binding domain containing 2; Zinc finger A20 domain-containing protein 1; Zinc finger protein Cezanne; zinc finger, A20 domain containing 1

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Gene Aliases: 2900060B22Rik; 4930463P07Rik; AI462125; CEZANNE; OTUD7B; RGD1308111; Trabid2; ZA20D1

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UniProt ID: (Human) Q6GQQ9, (Mouse) B2RUR8

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Entrez Gene ID: (Human) 56957, (Rat) 310677, (Mouse) 229603

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Function(s)

DNA binding thiol-dependent ubiquitin-specific protease activity protein binding cysteine-type peptidase activity zinc ion binding K63-linked polyubiquitin binding Lys48-specific deubiquitinase activity peptidase activity hydrolase activity metal ion binding cysteine protease

Process(es)

negative regulation of transcription from RNA polymerase II promoter mucosal immune response immune response negative regulation of interleukin-8 production protein K11-linked deubiquitination negative regulation of I-kappaB kinase/NF-kappaB signaling protein K63-linked deubiquitination protein K48-linked deubiquitination protein deubiquitination involved in ubiquitin-dependent protein catabolic process negative regulation of protein localization to nucleus proteolysis protein deubiquitination immune system process

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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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OTUD7B Polyclonal Antibody

OTUD7B Antibody (16605-1-AP) in IHC (P)

Product Details

16605-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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