Proteintech

OTUD7B Polyclonal Antibody

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OTUD7B Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded mouse kidney using 16605-1-AP (OTUD7B antibody) at dilution of 1:50 (under 40x lens).

OTUD7B Antibody in Western Blot (WB) — Jurkat cells were subjected to SDS PAGE followed by western blot with 16605-1-AP (OTUD7B antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

OTUD7B Antibody in Immunoprecipitation (IP) — IP result of anti-OTUD7B (IP:16605-1-AP, 4ug; Detection:16605-1-AP 1:800) with Jurkat cells lysate 2500ug.

Product Details

16605-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

View 10 publications 10 publications

Immunohistochemistry (IHC)

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:500

Immunocytochemistry (ICC/IF)

View 6 publications 6 publications

Immunoprecipitation (IP)

0.5-4.0 µg

View 2 publications 2 publications

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

OTUD7B Fusion Protein Ag9788 (1-355 aa encoded by BC020622)

View immunogen

Conjugate

Unconjugated

Form

Liquid

Concentration

0.22 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Product Specific Information

Immunogen sequence: MTLDMDAVL SDFVRSTGAE PGLARDLLEG KNWDVNAALS DFEQLRQVHA GNLPPSFSEG SGGSRTPEKG FSDREPTRPP RPILQRQDDI VQEKRLSRGI SHASSSIVSL ARSHVSSNGG GGGSNEHPLE MPICAFQLPD LTVYNEDFRS FIERDLIEQS MLVALEQAGR LNWWVSVDPT SQRLLPLATT GDGNCLLHAA SLGMWGFHDR DLMLRKALYA LMEKGVEKEA LKRRWRWQQT QQNEESGLVY TEDEWQKEWN ELIKLASSEP RMHLGTNGAN CGGVESSEEP VYESLEEFHV FVLAHVLRRP IVVVADTMLR DSGGEAFAPI PFGGIYLPLE VPASQCHRSP LVLAYD (1-355 aa encoded by B C020622)

Target Information

Negative regulator of the non-canonical NF-kappa-B pathway that acts by mediating deubiquitination of TRAF3, an inhibitor of the NF-kappa-B pathway, thereby acting as a negative regulator of B-cell responses. In response to non-canonical NF-kappa-B stimuli, deubiquitinates 'Lys-48'-linked polyubiquitin chains of TRAF3, preventing TRAF3 proteolysis and over-activation of non-canonical NF-kappa-B. Negatively regulates mucosal immunity against infections. Deubiquitinates ZAP70, and thereby regulates T cell receptor (TCR) signaling that leads to the activation of NF-kappa-B (PubMed:26903241). Plays a role in T cell homeostasis and is required for normal T cell responses, including production of IFNG and IL2. Mediates deubiquitination of EGFR (PubMed:22179831). Has deubiquitinating activity toward 'Lys-11', 'Lys-48' and 'Lys-63'-linked polyubiquitin chains (PubMed:27732584). Has a much higher catalytic rate with 'Lys-11'-linked polyubiquitin chains (in vitro); however the physiological significance of these data are unsure (PubMed:27732584). Hydrolyzes both linear and branched forms of polyubiquitin. [UniProt]

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Differential activity of transcription factor Sox9 in early and adult oligodendroglial progenitor cells.

Glia

Hassel LA,Fröb F,Küspert M,Hillgärtner S,Arnold P,Huang W,Kirchhoff F,Wegner M

Tue Aug 01 00:00:00 EDT 2023

AtomNet-Aided OTUD7B Inhibitor Discovery and Validation.

Cancers

Chen J,Bolhuis DL,Laggner C,Kong D,Yu L,Wang X,Emanuele MJ,Brown NG,Liu P

Sat Jan 14 00:00:00 EST 2023

Transcranial direct-current stimulation protects against cerebral ischemia-reperfusion injury through regulating Cezanne-dependent signaling.

Experimental neurology

Cheng J,Fan YQ,Jiang HX,Chen SF,Chen J,Liao XY,Zou YY,Lan HY,Cui Y,Chen ZB,Chen QX,Wan Q

Mon Nov 01 00:00:00 EDT 2021

OTUD7B Deubiquitinates LSD1 to Govern Its Binding Partner Specificity, Homeostasis, and Breast Cancer Metastasis.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)

Gong Z,Li A,Ding J,Li Q,Zhang L,Li Y,Meng Z,Chen F,Huang J,Zhou D,Hu R,Ye J,Liu W,You H

Sun Aug 01 00:00:00 EDT 2021

Salvia miltiorrhiza-derived miRNAs suppress vascular remodeling through regulating OTUD7B/KLF4/NMHC IIA axis.

Theranostics

Yang GS,Zheng B,Qin Y,Zhou J,Yang Z,Zhang XH,Zhao HY,Yang HJ,Wen JK

Wed May 05 00:00:00 EDT 2021

Salvia miltiorrhiza-derived miRNAs suppress vascular remodeling through regulating OTUD7B/KLF4/NMHC IIA axis.

Theranostics

Yang GS,Zheng B,Qin Y,Zhou J,Yang Z,Zhang XH,Zhao HY,Yang HJ,Wen JK

Wed May 05 00:00:00 EDT 2021

Salvia miltiorrhiza-derived miRNAs suppress vascular remodeling through regulating OTUD7B/KLF4/NMHC IIA axis.

Theranostics

Yang GS,Zheng B,Qin Y,Zhou J,Yang Z,Zhang XH,Zhao HY,Yang HJ,Wen JK

Wed May 05 00:00:00 EDT 2021

Salvia miltiorrhiza-derived miRNAs suppress vascular remodeling through regulating OTUD7B/KLF4/NMHC IIA axis.

Theranostics

Yang GS,Zheng B,Qin Y,Zhou J,Yang Z,Zhang XH,Zhao HY,Yang HJ,Wen JK

Wed May 05 00:00:00 EDT 2021

Cezanne contributes to cancer progression by playing a key role in the deubiquitination of IGF-1R.

American journal of cancer research

Chen X,Pang Z,Wang Y,Zhu L,Liu J,Du J

Mon Jan 11 00:00:00 EST 2021

The Clock-Controlled lncRNA-AK028245 Participates in the Immune Response via Immune Response Factors OTUD7B and A20.

Journal of biological rhythms

Qi F,Jiang Z,Hou W,Peng B,Cheng S,Zhang X,Luo Z,Dai Z,Wang Y,Liu Y,Wang Y,Wang Z

Tue Dec 01 00:00:00 EST 2020

Upregulation of OTUD7B (Cezanne) Promotes Tumor Progression via AKT/VEGF Pathway in Lung Squamous Carcinoma and Adenocarcinoma.

Frontiers in oncology

Lin DD,Shen Y,Qiao S,Liu WW,Zheng L,Wang YN,Cui N,Wang YF,Zhao S,Shi JH

Thu Oct 01 00:00:00 EDT 2020

Dynamic ubiquitylation of Sox2 regulates proteostasis and governs neural progenitor cell differentiation.

Nature communications

Cui CP,Zhang Y,Wang C,Yuan F,Li H,Yao Y,Chen Y,Li C,Wei W,Liu CH,He F,Liu Y,Zhang L

Wed Nov 07 00:00:00 EST 2018

Dynamic ubiquitylation of Sox2 regulates proteostasis and governs neural progenitor cell differentiation.

Nature communications

Cui CP,Zhang Y,Wang C,Yuan F,Li H,Yao Y,Chen Y,Li C,Wei W,Liu CH,He F,Liu Y,Zhang L

Wed Nov 07 00:00:00 EST 2018

The N-terminal ubiquitin-associated domain of Cezanne is crucial for its function to suppress NF-κB pathway.

Journal of cellular biochemistry

Ji Y,Cao L,Zeng L,Zhang Z,Xiao Q,Guan P,Chen S,Chen Y,Wang M,Guo D

Thu Feb 01 00:00:00 EST 2018

The N-terminal ubiquitin-associated domain of Cezanne is crucial for its function to suppress NF-κB pathway.

Journal of cellular biochemistry

Ji Y,Cao L,Zeng L,Zhang Z,Xiao Q,Guan P,Chen S,Chen Y,Wang M,Guo D

Thu Feb 01 00:00:00 EST 2018

The N-terminal ubiquitin-associated domain of Cezanne is crucial for its function to suppress NF-κB pathway.

Journal of cellular biochemistry

Ji Y,Cao L,Zeng L,Zhang Z,Xiao Q,Guan P,Chen S,Chen Y,Wang M,Guo D

Thu Feb 01 00:00:00 EST 2018

The N-terminal ubiquitin-associated domain of Cezanne is crucial for its function to suppress NF-κB pathway.

Journal of cellular biochemistry

Ji Y,Cao L,Zeng L,Zhang Z,Xiao Q,Guan P,Chen S,Chen Y,Wang M,Guo D

Thu Feb 01 00:00:00 EST 2018

Mechanical Activation of Hypoxia-Inducible Factor 1α Drives Endothelial Dysfunction at Atheroprone Sites.

Arteriosclerosis, thrombosis, and vascular biology

Feng S,Bowden N,Fragiadaki M,Souilhol C,Hsiao S,Mahmoud M,Allen S,Pirri D,Ayllon BT,Akhtar S,Thompson AAR,Jo H,Weber C,Ridger V,Schober A,Evans PC

Wed Nov 01 00:00:00 EDT 2017

Mechanical Activation of Hypoxia-Inducible Factor 1α Drives Endothelial Dysfunction at Atheroprone Sites.

Arteriosclerosis, thrombosis, and vascular biology

Feng S,Bowden N,Fragiadaki M,Souilhol C,Hsiao S,Mahmoud M,Allen S,Pirri D,Ayllon BT,Akhtar S,Thompson AAR,Jo H,Weber C,Ridger V,Schober A,Evans PC

Wed Nov 01 00:00:00 EDT 2017

Mechanical Activation of Hypoxia-Inducible Factor 1α Drives Endothelial Dysfunction at Atheroprone Sites.

Arteriosclerosis, thrombosis, and vascular biology

Feng S,Bowden N,Fragiadaki M,Souilhol C,Hsiao S,Mahmoud M,Allen S,Pirri D,Ayllon BT,Akhtar S,Thompson AAR,Jo H,Weber C,Ridger V,Schober A,Evans PC

Wed Nov 01 00:00:00 EDT 2017

Mechanical Activation of Hypoxia-Inducible Factor 1α Drives Endothelial Dysfunction at Atheroprone Sites.

Arteriosclerosis, thrombosis, and vascular biology

Feng S,Bowden N,Fragiadaki M,Souilhol C,Hsiao S,Mahmoud M,Allen S,Pirri D,Ayllon BT,Akhtar S,Thompson AAR,Jo H,Weber C,Ridger V,Schober A,Evans PC

Wed Nov 01 00:00:00 EDT 2017

Otud7b facilitates T cell activation and inflammatory responses by regulating Zap70 ubiquitination.

The Journal of experimental medicine

Hu H,Wang H,Xiao Y,Jin J,Chang JH,Zou Q,Xie X,Cheng X,Sun SC

Mon Mar 07 00:00:00 EST 2016

Otud7b facilitates T cell activation and inflammatory responses by regulating Zap70 ubiquitination.

The Journal of experimental medicine

Hu H,Wang H,Xiao Y,Jin J,Chang JH,Zou Q,Xie X,Cheng X,Sun SC

Mon Mar 07 00:00:00 EST 2016

Cezanne regulates inflammatory responses to hypoxia in endothelial cells by targeting TRAF6 for deubiquitination.

Circulation research

Luong le A,Fragiadaki M,Smith J,Boyle J,Lutz J,Dean JL,Harten S,Ashcroft M,Walmsley SR,Haskard DO,Maxwell PH,Walczak H,Pusey C,Evans PC

Fri Jun 07 00:00:00 EDT 2013

Cezanne regulates inflammatory responses to hypoxia in endothelial cells by targeting TRAF6 for deubiquitination.

Circulation research

Luong le A,Fragiadaki M,Smith J,Boyle J,Lutz J,Dean JL,Harten S,Ashcroft M,Walmsley SR,Haskard DO,Maxwell PH,Walczak H,Pusey C,Evans PC

Fri Jun 07 00:00:00 EDT 2013

OTUD7B controls non-canonical NF-κB activation through deubiquitination of TRAF3.

Nature

Hu H,Brittain GC,Chang JH,Puebla-Osorio N,Jin J,Zal A,Xiao Y,Cheng X,Chang M,Fu YX,Zal T,Zhu C,Sun SC

Thu Feb 21 00:00:00 EST 2013

OTUD7B controls non-canonical NF-κB activation through deubiquitination of TRAF3.

Nature

Hu H,Brittain GC,Chang JH,Puebla-Osorio N,Jin J,Zal A,Xiao Y,Cheng X,Chang M,Fu YX,Zal T,Zhu C,Sun SC

Thu Feb 21 00:00:00 EST 2013

OTUD7B controls non-canonical NF-κB activation through deubiquitination of TRAF3.

Nature

Hu H,Brittain GC,Chang JH,Puebla-Osorio N,Jin J,Zal A,Xiao Y,Cheng X,Chang M,Fu YX,Zal T,Zhu C,Sun SC

Thu Feb 21 00:00:00 EST 2013

OTUD7B controls non-canonical NF-κB activation through deubiquitination of TRAF3.

Nature

Hu H,Brittain GC,Chang JH,Puebla-Osorio N,Jin J,Zal A,Xiao Y,Cheng X,Chang M,Fu YX,Zal T,Zhu C,Sun SC

Thu Feb 21 00:00:00 EST 2013

Bioinformatics

Protein Aliases: Cellular zinc finger anti-NF-kappa-B protein; cellular zinc finger anti-NF-kappaB Cezanne; Cezanne; OTU domain containing 7B; OTU domain-containing protein 7B; TRAF binding domain containing 2; Zinc finger A20 domain-containing protein 1; Zinc finger protein Cezanne; zinc finger, A20 domain containing 1

Gene Aliases: 2900060B22Rik; 4930463P07Rik; AI462125; CEZANNE; OTUD7B; RGD1308111; Trabid2; ZA20D1

UniProt ID: (Human) Q6GQQ9, (Mouse) B2RUR8

Entrez Gene ID: (Human) 56957, (Rat) 310677, (Mouse) 229603

Molecular Function: cysteine protease