Invitrogen

OX40L Polyclonal Antibody

View all (17) OX40L (CD252) antibodies

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OX40L Antibody in Immunohistochemistry (IHC) — Immunofluorescent analysis of human spleen tissue using a CD252/OX40L polyclonal antibody (Product # PA5-34516) at a 20 µg/mL dilution.

OX40L Antibody in Immunohistochemistry (IHC) — Immunohistochemical staining of spleen tissue using a CD252/OX40L polyclonal antibody (Product # PA5-34516) at a 5 µg/mL dilution.

OX40L Antibody in Western Blot (WB) — Western blot analysis of rat spleen tissue lysate using a CD252/OX40L polyclonal antibody (Product # PA5-34516) at (A) 0.5 and (B) 1 µg/mL

Product Details

Tested Applications

Dilution

Immunocytochemistry (ICC)

20 µg/mL

Immunofluorescence (IF)

20 µg/mL

Immunohistochemistry (IHC)

5 µg/mL

Western Blot (WB)

0.5-1 µg/mL

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host / Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A 14 amino acid peptide near the carboxy terminus of human TNFSF4

Conjugate

Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS

Contains

0.02% sodium azide

Storage conditions

Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C

RRID

AB_2551868

Product Specific Information

A suggested positive control is rat spleen tissue lysate.

PA5-34516 can be used with blocking peptide PEP-1559.

Target Information

The tumor necrosis factor superfamily member TNFSF4 is a type II membrane bound, non-covalently linked homotrimeric protein. It is expressed on antigen presenting cells, such as dendritic cells and activated B-cells, and also on other cells such as vascular endothelial cells, mast cells, and natural killer cells. TNFSF4 signals specifically through the TNFRSF4 receptor, is expressed predominantly on CD4+T cells but also on certain activated CD8+T cells. TNFRSF4/TNFSF4 functions as a costimulatory signal, which is required for a productive interaction between antigen presenting cells and their target T-cells. It enhances cell proliferation and survival, and increases expression of RANTES, IL-2, IL-3, and IFNgamma. TNFRSF4/TNFSF4 signaling plays an important role in immunotolerance.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: Ath-1; Ath1; atherosclerosis 1; CD134 ligand; CD252; Glycoprotein Gp34; gp34; OX40 antigen ligand; OX40 Ligand; OX40L; TAX transcriptionally-activated glycoprotein 1; tax-transcriptionally activated glycoprotein 1 (34kD); tax-transcriptionally activated glycoprotein 1 ligand; TNFSF4; tumor necrosis factor (ligand) superfamily member 4; tumor necrosis factor ligand 2B; Tumor necrosis factor ligand superfamily member 4; TXGP1; Txgp1l

Gene Aliases: Ath-1; Ath1; CD134L; CD252; GP34; OX-40L; Ox40l; OX4OL; TNFSF4; TNLG2B; TXGP1; Txgp1l

UniProt ID: (Human) P23510, (Rat) Q9Z2P3, (Mouse) P43488

Entrez Gene ID: (Human) 7292, (Rat) 89814, (Mouse) 22164

Molecular Function: cytokine signaling molecule tumor necrosis factor family member

defense response to nematode acute inflammatory response positive regulation of T cell cytokine production regulation of adaptive immune response positive regulation of type 2 immune response positive regulation of immunoglobulin mediated immune response immune response signal transduction cholesterol metabolic process positive regulation of cell proliferation response to virus negative regulation of interferon-gamma production negative regulation of interleukin-17 production positive regulation of interferon-gamma production positive regulation of interleukin-10 production positive regulation of interleukin-12 production positive regulation of interleukin-13 production positive regulation of interleukin-2 production positive regulation of interleukin-4 production positive regulation of interleukin-6 production tumor necrosis factor-mediated signaling pathway memory T cell activation T-helper 2 cell activation response to nitrogen dioxide cellular response to nitrogen dioxide CD4-positive, alpha-beta T cell costimulation T cell proliferation positive regulation of T cell proliferation positive regulation of activated T cell proliferation positive regulation of CD4-positive, alpha-beta T cell differentiation positive regulation of memory T cell differentiation negative regulation of sequence-specific DNA binding transcription factor activity negative regulation of regulatory T cell differentiation negative regulation of T-helper 1 cell differentiation positive regulation of T-helper 2 cell differentiation negative regulation of transcription, DNA-templated positive regulation of alpha-beta T cell proliferation negative regulation of cytokine secretion regulation of inflammatory response positive regulation of inflammatory response positive regulation of B cell activation positive regulation of immunoglobulin secretion cellular response to lipopolysaccharide cellular response to prostaglandin E stimulus chemokine (C-C motif) ligand 11 production positive regulation of CD4-positive, alpha-beta T cell costimulation positive regulation of memory T cell activation positive regulation of T-helper 2 cell activation positive regulation of interleukin-4-dependent isotype switching to IgE isotypes cytokine production inflammatory response positive regulation of T cell costimulation blood vessel development positive regulation of immune effector process innate immune response negative regulation of T cell apoptotic process negative regulation of activation-induced cell death of T cells positive regulation of T cell migration negative regulation of extrinsic apoptotic signaling pathway

Process(es)

receptor binding cytokine activity tumor necrosis factor receptor binding tumor necrosis factor receptor superfamily binding