Invitrogen

OX40L Polyclonal Antibody

View all (19) OX40L (CD252) antibodies

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OX40L Antibody in Immunocytochemistry (ICC/IF) — Immunocytochemistry analysis of OX40L in immersion fixed mouse splenocytes. Samples were incubated in OX40L polyclonal antibody (Product # PA5-47100) using a dilution of 15 µg/mL for 3 hours at room temperature followed by NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red) and counterstained with DAPI (blue). Specific staining was localized to the plasma membrane and cytoplasm.

OX40L Antibody in Neutralization (Neu) — Neutralization of OX40L in mouse T cells. Samples were incubated in OX40L polyclonal antibody (Product # PA5-47100) using a dilution of 100 ng/mL. In the presence of a cross-linking antibody, Mouse polyHistidine Monoclonal Antibody (10 µg/mL) and sub-optimal amounts of Mouse CD3ε Monoclonal Antibody, Recombinant Mouse OX40 Ligand/TNFSF4 stimulates IL‚2 secretion in mouse T cells in a dose-dependent manner (orange line), as measured by the Mouse IL‚2 Quantikine ELISA Kit. Under these conditions, IL‚2 secretion elicited by Recombinant Mouse OX40 Ligand/TNFSF4 is neutralized (green line) by increasing concentrations of Goat Anti-Mouse OX40 Ligand/TNFSF4 Antigen Affinity-purified Polyclonal Antibody. The ND50 is typically 0.2-0.6 µg/mL.

Product Details

PA5-47100

Applications	Tested Dilution	Publications
Western Blot (WB)	0.1 µg/mL	-
Immunocytochemistry (ICC/IF)	5-15 µg/mL	-
Neutralization (Neu)	0.2-0.6 µg/mL	-

Product Specifications
Species Reactivity	Mouse
Host/Isotype	Goat / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Mouse myeloma cell line NS0-derived recombinant mouse OX40 Ligand/TNFSF4 Gln49-Leu198 View antigen
Conjugate	Unconjugated
Form	Lyophilized
Concentration	0.2 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS with 5% trehalose
Contains	No Preservative
Storage conditions	-20° C, Avoid Freeze/Thaw Cycles
RRID	AB_2608758

Product Specific Information

In Western blots, approximately 5% cross-reactivity with recombinant human (rh) OX40 Ligand, rhLIGHT, recombinant mouse (rm) Fas Ligand and less than 1% cross-reactivity with rmTNF- alpha, rhGITR Ligand, rhTRANCE, and rhVEGI is observed.

Reconstitute at 0.2 mg/mL in sterile PBS.

Endoxin level is <0.10 EU per 1 µg of the antibody by the LAL method.

Target Information

The tumor necrosis factor superfamily member TNFSF4 is a type II membrane bound, non-covalently linked homotrimeric protein. It is expressed on antigen presenting cells, such as dendritic cells and activated B-cells, and also on other cells such as vascular endothelial cells, mast cells, and natural killer cells. TNFSF4 signals specifically through the TNFRSF4 receptor, is expressed predominantly on CD4+T cells but also on certain activated CD8+T cells. TNFRSF4/TNFSF4 functions as a costimulatory signal, which is required for a productive interaction between antigen presenting cells and their target T-cells. It enhances cell proliferation and survival, and increases expression of RANTES, IL-2, IL-3, and IFNgamma. TNFRSF4/TNFSF4 signaling plays an important role in immunotolerance.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: atherosclerosis 1; CD252; OX40 ligand; OX40L; tax-transcriptionally activated glycoprotein 1 ligand; Tumor necrosis factor ligand superfamily member 4

Gene Aliases: Ath-1; Ath1; CD134L; gp34; OX-40L; Ox40l; Tnfsf4; TXGP1; Txgp1l

UniProt ID: (Mouse) P43488

Entrez Gene ID: (Mouse) 22164

blood vessel development cytokine production defense response to nematode acute inflammatory response positive regulation of immune effector process positive regulation of T cell cytokine production regulation of adaptive immune response positive regulation of type 2 immune response positive regulation of immunoglobulin mediated immune response inflammatory response immune response cholesterol metabolic process response to virus negative regulation of interferon-gamma production negative regulation of interleukin-17 production positive regulation of interferon-gamma production positive regulation of interleukin-10 production positive regulation of interleukin-12 production positive regulation of interleukin-13 production positive regulation of interleukin-2 production positive regulation of interleukin-4 production positive regulation of interleukin-6 production memory T cell activation T-helper 2 cell activation response to nitrogen dioxide cellular response to nitrogen dioxide CD4-positive, alpha-beta T cell costimulation T cell proliferation positive regulation of activated T cell proliferation positive regulation of CD4-positive, alpha-beta T cell differentiation positive regulation of memory T cell differentiation negative regulation of sequence-specific DNA binding transcription factor activity innate immune response negative regulation of regulatory T cell differentiation negative regulation of T-helper 1 cell differentiation positive regulation of T-helper 2 cell differentiation negative regulation of transcription, DNA-templated positive regulation of alpha-beta T cell proliferation negative regulation of cytokine secretion regulation of inflammatory response positive regulation of inflammatory response positive regulation of B cell activation positive regulation of immunoglobulin secretion negative regulation of T cell apoptotic process negative regulation of activation-induced cell death of T cells cellular response to lipopolysaccharide cellular response to prostaglandin E stimulus chemokine (C-C motif) ligand 11 production positive regulation of CD4-positive, alpha-beta T cell costimulation positive regulation of T cell migration positive regulation of T cell costimulation positive regulation of memory T cell activation positive regulation of T-helper 2 cell activation positive regulation of interleukin-4-dependent isotype switching to IgE isotypes negative regulation of extrinsic apoptotic signaling pathway

Process(es)

cytokine activity tumor necrosis factor receptor binding tumor necrosis factor receptor superfamily binding