Invitrogen

OX40L (CD252) Polyclonal Antibody

View all (17) OX40L (CD252) antibodies

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OX40L (CD252) Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of OX40L (CD252) on paraffin-embedded rat small intestine tissue. Antigen retrieval was performed using citrate buffer (pH6, epitope retrieval solution) for 20 mins. Sample was blocked using 10% goat serum, incubated with OX40L (CD252) polyclonal antibody (Product# PA5-80169) with a dilution of 1 µg/mL (overnight at 4°C), and followed by biotinylated goat anti-rabbit IgG (30 minutes at 37°C). Development was performed using Streptavidin-Biotin-Complex (SABC) with DAB chromogen method.

OX40L (CD252) Antibody in Western Blot (WB) — Western blot analysis of OX40L (CD252) in Lane 1: recombinant mouse CD252 protein using 1 ng per well. Electrophoresis was performed on 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours and protein was transferred to a nitrocellulose membrane at 150mA for 50-90 minutes. Sample was blocked with 5% Non-fat Milk/TBS for 1.5 hours at room temperature, incubated with OX40L (CD252) polyclonal antibody (Product # PA5-80169) at a dilution of 0.5 µg/mL (overnight at 4°C), followed by goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10,000. Signal development was performed using a chemiluminescence (ECL) kit.

Product Details

Tested Applications

Dilution

ELISA (ELISA)

1-5 µg/mL (capture)

Western Blot (WB)

0.1-0.5 µg/mL

Product Specifications

Species Reactivity

Mouse

Host / Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

E. coli-derived mouse CD252 recombinant protein (Position: Q49-L198).

Conjugate

Unconjugated

Form

Lyophilized

Purification

Antigen affinity chromatography

Storage buffer

PBS with 4mg trehalose

Contains

0.05mg sodium azide

Storage conditions

-20°C

RRID

AB_2747283

Product Specific Information

Add 0.2 mL of distilled water, will yield a concentration of 500 µg/mL.

Target Information

OX40L (TNFSF4) is the ligand for CD134 and is expressed on such cells as DC2s (a subtype of dendritic cells) enabling amplification of Th2 cell differentiation. This gene encodes a cytokine of the tumor necrosis factor (TNF) ligand family. The encoded protein functions in T cell antigen-presenting cell (APC) interactions and mediates adhesion of activated T cells to endothelial cells. Polymorphisms in this gene have been associated with Sjogren's syndrome and systemic lupus erythematosus. Alternative splicing results in multiple transcript variants.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: atherosclerosis 1; CD252; OX40 ligand; OX40L; tax-transcriptionally activated glycoprotein 1 ligand; Tnfsf4; Tumor necrosis factor ligand superfamily member 4; Txgp1l

Gene Aliases: Ath-1; Ath1; CD134L; gp34; OX-40L; Ox40l; Tnfsf4; TXGP1; Txgp1l

UniProt ID: (Mouse) P43488

Entrez Gene ID: (Mouse) 22164

Molecular Function: cytokine signaling molecule tumor necrosis factor family member

blood vessel development cytokine production defense response to nematode acute inflammatory response positive regulation of immune effector process positive regulation of T cell cytokine production regulation of adaptive immune response positive regulation of type 2 immune response positive regulation of immunoglobulin mediated immune response inflammatory response immune response cholesterol metabolic process response to virus negative regulation of interferon-gamma production negative regulation of interleukin-17 production positive regulation of interferon-gamma production positive regulation of interleukin-10 production positive regulation of interleukin-12 production positive regulation of interleukin-13 production positive regulation of interleukin-2 production positive regulation of interleukin-4 production positive regulation of interleukin-6 production memory T cell activation T-helper 2 cell activation response to nitrogen dioxide cellular response to nitrogen dioxide CD4-positive, alpha-beta T cell costimulation T cell proliferation positive regulation of activated T cell proliferation positive regulation of CD4-positive, alpha-beta T cell differentiation positive regulation of memory T cell differentiation negative regulation of sequence-specific DNA binding transcription factor activity innate immune response negative regulation of regulatory T cell differentiation negative regulation of T-helper 1 cell differentiation positive regulation of T-helper 2 cell differentiation negative regulation of transcription, DNA-templated positive regulation of alpha-beta T cell proliferation negative regulation of cytokine secretion regulation of inflammatory response positive regulation of inflammatory response positive regulation of B cell activation positive regulation of immunoglobulin secretion negative regulation of T cell apoptotic process negative regulation of activation-induced cell death of T cells cellular response to lipopolysaccharide cellular response to prostaglandin E stimulus chemokine (C-C motif) ligand 11 production positive regulation of CD4-positive, alpha-beta T cell costimulation positive regulation of T cell migration positive regulation of T cell costimulation positive regulation of memory T cell activation positive regulation of T-helper 2 cell activation positive regulation of interleukin-4-dependent isotype switching to IgE isotypes negative regulation of extrinsic apoptotic signaling pathway

Process(es)

cytokine activity tumor necrosis factor receptor binding tumor necrosis factor receptor superfamily binding