|Immunocytochemistry (ICC)||1 µg/mL|
|Immunofluorescence (IF)||1-2 µg/mL|
|Immunohistochemistry (Paraffin) (IHC (P))||6 µg/mL|
|Western Blot (WB)||1-3 µg/mL|
|Western Blot (WB)||See 9 publications below|
|Immunohistochemistry (Paraffin) (IHC (P))||See 2 publications below|
|Immunohistochemistry (Frozen) (IHC (F))||See 1 publications below|
|Immunohistochemistry (IHC)||See 4 publications below|
|Immunocytochemistry (ICC)||See 3 publications below|
|Miscellaneous PubMed (MISC)||See 2 publications below|
|Immunofluorescence (IF)||See 2 publications below|
|Immunoprecipitation (IP)||See 1 publications below|
|Tested Species reactivity||Dog, Human, Mouse, Rat|
|Published species reactivity||Dog , Rat , Pig , Mouse , Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide derived from the C-terminal region of the human occludin protein|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.4|
|Contains||0.1% sodium azide|
This antibody is specific for the human occludin protein. On western blots, it identifies the target band at ~65 kDa.
Reactivity has been confirmed with human Caco-2 and HT29, dog MDCK, mouse TCMK, and rat KNRK cell lysates, and mouse kidney, liver, and rat liver homogenates by western blotting, Caco-2 cells by immunofluorescence, and paraffin-embedded human small intestine tissue by immunohistochemistry.
For immunofluorescence using Caco-2 cells, fixation with cold ethanol is recommended. For immunohistochemistry in paraffin-embedded tissues, enzyme digestion with pepsin is required prior to staining.
Tight junction group of proteins are a cell-to-cell adhesion structure in epithelial cells that constitute the epithelial junctional complex with adherens junctions and desmosomes. Tight junction strands are mainly composed of claudins, occludin, and junction adhesion molecules (JAMs). Occludin is a 65 kDa protein and is considered to be vital in the assembly and maintenance of tight junctions. At the tight junction, occludin associates with membrane peripheral protein ZO-1. Occludin can exist in a variety of phosphorylated forms, ranging up to approximately 82 kDa. This phosphorylation is thought to be involved in regulating both the localization and the function of occludin. The occludin protein was first identified in chicken using monoclonal antibodies. The chicken occludin cDNA was subsequently cloned and sequenced, and the amino acid sequence revealed that the occludin protein is organized into five distinct domains: a short N-terminal cytoplasmic domain (domain A), two extracellular loops (domains B and D) separated by a short intracellular loop (domain C), and a long C-terminal cytoplasmic tail (domain E). The C-terminal tail of occludin is required for both of its localization at tight junctions and for its direct interaction with the ZO-1 protein. The expression of occludin varies greatly on the type of tissue. It is continuously and highly expressed in brain whereas nonneural tissues show lower expression and discontinuous distribution. Underexpression of tight junction proteins, including occludin, is a key molecular abnormality responsible for the increased permeability of tumor endothelial tight junctions, which contributes to brain tumor edemas. Polyunsaturated fatty acids are known to upregulate occludin expression, increasing the transendothelial cell resistance and reducing the cellular permeability to large molecules. One interesting feature of the occluding protein is that its amino acid sequence has not been highly conserved throughout evolution. This fact made isolating the mammalian homologues of chicken occludin a rather difficult task. Recently, however, the sequences of the full-length cDNAs encoding occludin of rat-kangaroo, human, mouse, and dog were reported. At the amino acid level, the human, murine, and canine occludin proteins are highly homologous (~ 90% identity); however, the mammalian proteins exhibit a considerable degree of divergence from the rat-kangaroo and chicken proteins. Nevertheless, the overall structural features of the occludin protein are highly conserved in all the species examined. The recent identification and cloning of the mammalian occludin protein will undoubtedly facilitate the further study of TJ organization and function.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Occludin; OCLN; phosphatase 1, regulatory subunit 115; tight junction protein occludin
Gene Aliases: AI503564; BLCPMG; Ocl; OCLN; PPP1R115
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