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Optineurin Antibody (PA5-28249) in ICC/IF
Immunofluorescence analysis of Optineurin was performed using 70 percent confluent log phase A-431 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with Optineurin Polyclonal Antibody (Product # PA5-28249) at 1:200 in 0.1% BSA, incubated at 4 degree cel... View More
Product Details
PA5-28249
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein fragment corresponding to a region within amino acids 194 and 558 of Human Optineurin
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
0.1M tris glycine, pH 7, with 10% glycerol
Contains
0.01% thimerosal
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2545725
Product Specific Information
Recommended positive controls: 293T, A431, H1299, HeLaS3, HepG2, Molt-4, Raji.
Predicted reactivity: Mouse (81%), Rat (82%), Pig (84%), Rhesus Monkey (96%), Bovine (86%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
This gene encodes the coiled-coil containing protein optineurin. Optineurin may play a role in normal-tension glaucoma and adult-onset primary open angle glaucoma. Optineurin interacts with adenovirus E3-14. 7K protein and may utilize tumor necrosis factor-alpha or Fas-ligand pathways to mediate apoptosis, inflammation or vasoconstriction. Optineurin may also function in cellular morphogenesis and membrane trafficking, vesicle trafficking, and transcription activation through its interactions with the RAB8, huntingtin, and transcription factor IIIA proteins. Alternative splicing results in multiple transcript variants encoding the same protein.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Ag9 C5; E3-14.7K-interacting protein; FIP 2; FIP-2; HIP 7; HIP-7; Huntingtin interacting protein L; Huntingtin yeast partner L; Huntingtin-interacting protein 7; Huntingtin-interacting protein L; NEMO-related protein; Optic neuropathy-inducing protein; Optineurin; TFIIIA IntP; TFIIIA-IntP; Transcription factor IIIA-interacting protein; transcrption factor IIIA-interacting protein; tumor necrosis factor alpha-inducible cellular protein containing leucine zipper domains; Uban
Gene Aliases: ALS12; FIP2; GLC1E; HIP7; HYPL; NRP; OPTN; TFIIIA-INTP
UniProt ID: (Human) Q96CV9
Entrez Gene ID: (Human) 10133
protein targeting to Golgi
G2/M transition of mitotic cell cycle
negative regulation of receptor recycling
Golgi organization
signal transduction
cell death
macroautophagy
Golgi to plasma membrane protein transport
regulation of I-kappaB kinase/NF-kappaB signaling
negative regulation of I-kappaB kinase/NF-kappaB signaling
defense response to Gram-negative bacterium
parkin-mediated mitophagy in response to mitochondrial depolarization
Golgi ribbon formation
xenophagy
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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