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Orexin Receptor 2 Antibody (AOR-002-200UL) in ICC/IF
Expression of OX2R in human colon cancer cells - Immunocytochemical staining of paraformaldehyde-fixed and permeabilized human Colo-205 colon cancer cells using Anti-Orexin Receptor 2 Antibody (#AOR-002), (1:500), followed by goat- Anti-rabbit-AlexaFluor-555 secondary Antibody (red). B.Live view of the same field as in (A). C.Nuclei were visualized with the cell permeable dye Hoechst 33342 (blue).
Product Details
AOR-002-200UL
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
(C)DRLARGRTSTESRKS, corresponding to amino acid residues 391-405 of rat OX2R, Intracellular, C-terminus
Conjugate
Form
Lyophilized
Concentration
0.8 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.4, with 1% BSA
Contains
0.05% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Reconstitution: 25 µL, 50 µL or 0.2 mL double distilled water (DDW), depending on the sample size. The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20C. The reconstituted solution can be stored at 4C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 X g 5 min).
Target Information
The protein encoded by this gene is a G-protein coupled receptor involved in the regulation of feeding behavior. The encoded protein binds the hypothalamic neuropeptides orexin A and orexin B. A related gene encodes a G-protein coupled receptor that selectively binds orexin A.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: HCRTR1; hypocretin (orexin) receptor 2; hypocretin receptor 2; Hypocretin receptor type 2; orexin receptor 1; orexin receptor 2; Orexin receptor type 2; orexin receptor-1; orexin receptor-2; orexin type-2 receptor; OTTHUMP00000039969; Ox-2-R; OX1R; ox2-R; ox2r
Gene Aliases: HCRTR2; mOX2aR; mOX2bR; Mox2r; mOXR2; OX2R
UniProt ID: (Human) O43614, (Rat) P56719, (Mouse) P58308
Entrez Gene ID: (Human) 3062, (Rat) 25605, (Mouse) 387285
phospholipase C-activating G-protein coupled receptor signaling pathway
neuropeptide signaling pathway
chemical synaptic transmission
feeding behavior
regulation of circadian sleep/wake cycle, wakefulness
circadian sleep/wake cycle process
cellular response to hormone stimulus
regulation of circadian sleep/wake cycle, sleep
regulation of cytosolic calcium ion concentration
response to peptide
G-protein coupled receptor signaling pathway
signal transduction
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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