Invitrogen

Osteocalcin Polyclonal Antibody

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Osteocalcin Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of Osteocalcin on paraffin-embedded rat tibia tissue. Antigen retrieval was performed using citrate buffer (pH6, epitope retrieval solution) for 20 mins. Sample was blocked using 10% goat serum, incubated with Osteocalcin polyclonal antibody (Product# PA5-78871) with a dilution of 1 µg/mL (overnight at 4°C), and followed by biotinylated goat anti-rabbit IgG (30 minutes at 37°C). Development was performed using Streptavidin-Biotin-Complex (SABC) with DAB chromogen method.

Product Details

Tested Applications

Dilution

Flow Cytometry (Flow)

1-3 µg/1x10^6 cells

Immunocytochemistry (ICC)

0.5-1 µg/mL

Immunofluorescence (IF)

0.5-1 µg/mL

Immunohistochemistry (Frozen) (IHC (F))

0.5-1 µg/mL

Immunohistochemistry (Paraffin) (IHC (P))

0.5-1 µg/mL

Product Specifications

Species Reactivity

Rat

Host / Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

E. coli-derived rat Osteocalcin recombinant protein (Position: Y50-V99).

Conjugate

Unconjugated

Form

Lyophilized

Purification

Antigen affinity chromatography

Storage buffer

PBS with 5mg BSA

Contains

0.05mg sodium azide

Storage conditions

-20°C

RRID

AB_2745987

Product Specific Information

Add 0.2 mL of distilled water, will yield a concentration of 500 µg/mL.

Target Information

Osteocalcin belongs to the osteocalcin/matrix Gla-protein family and constitutes 1-2% of the total bone protein. It binds strongly to apatite and calcium. Gamma-carboxyglutamate residues are formed by vitamin K dependent carboxylation. These residues are essential for the binding of calcium.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: Bglap; Bglap2; BGP; bone gamma-carboxyglutamate (gla) protein (osteocalcin); bone gamma-carboxyglutamate protein 2; bone gamma-carboxyglutamic acid (Gla) protein (osteocalcin); Bone Gla protein; bone Gla-protein; Gamma-carboxyglutamic acid-containing protein; Osteocalcin

Gene Aliases: Bglap; Bglap2; Bgp; Bgpr; Bgpra

UniProt ID: (Rat) P04640

Entrez Gene ID: (Rat) 25295

Molecular Function: calcium-binding protein extracellular matrix protein