Invitrogen

Osteocalcin Polyclonal Antibody

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Osteocalcin Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of Osteocalcin on paraffin-embedded rat tibia tissue. Antigen retrieval was performed using citrate buffer (pH6, epitope retrieval solution) for 20 mins. Sample was blocked using 10% goat serum, incubated with Osteocalcin polyclonal antibody (Product# PA5-78871) with a dilution of 1 µg/mL (overnight at 4°C), and followed by biotinylated goat anti-rabbit IgG (30 minutes at 37°C). Development was performed using Streptavidin-Biotin-Complex (SABC) with DAB chromogen method.

Product Details

PA5-78871

Applications	Tested Dilution	Publications
Immunohistochemistry (Paraffin) (IHC (P))	0.5-1 µg/mL	-
Immunohistochemistry (Frozen) (IHC (F))	0.5-1 µg/mL	-
Immunocytochemistry (ICC/IF)	0.5-1 µg/mL	-
Flow Cytometry (Flow)	1-3 µg/1x10^6 cells	-

Product Specifications
Species Reactivity	Rat
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	E. coli-derived rat Osteocalcin recombinant protein (Position: Y50-V99). View antigen
Conjugate	Unconjugated
Form	Lyophilized
Concentration	500 µg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS with 5mg BSA
Contains	0.05mg sodium azide
Storage conditions	-20°C
RRID	AB_2745987

Product Specific Information

Add 0.2 mL of distilled water, will yield a concentration of 500 µg/mL.

Target Information

Osteocalcin belongs to the osteocalcin/matrix Gla-protein family and constitutes 1-2% of the total bone protein. It binds strongly to apatite and calcium. Gamma-carboxyglutamate residues are formed by vitamin K dependent carboxylation. These residues are essential for the binding of calcium.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: BGP; bone gamma-carboxyglutamate (gla) protein (osteocalcin); bone gamma-carboxyglutamate protein 2; bone gamma-carboxyglutamic acid (Gla) protein (osteocalcin); Bone Gla protein; bone Gla-protein; Gamma-carboxyglutamic acid-containing protein; Osteocalcin; PMF1; RP11-54H19.5

Gene Aliases: Bglap; Bglap2; Bgp; Bgpr; Bgpra

UniProt ID: (Rat) P04640

Entrez Gene ID: (Rat) 25295

Function(s)