|Tested species reactivity||Mouse|
|Published species reactivity||Mouse|
|Host / Isotype||Goat / IgG|
|Immunogen||Recombinant, full length mouse protein, expressed in NSO cells.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS with 5% trehalose|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|ELISA (ELISA)||0.5-1 µg/ml|
|Inhibition Assays (IA)||1-3 ug/ml|
|Neutralization (Neu)||1-3 µg/ml|
|Western Blot (WB)||0.1-0.2 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunohistochemistry (IHC)||See 1 publications below|
PA1-25152 detects Osteopontin from mouse samples.
PA1-25152 has been successfully used in ELISA, neutralization and inhibition assays, and Western blot procedures. By Western Blot, a doublet band of approximately 65 kDa and a band of 30 kDa is observed due to glycosylation. A suggested positive control is 293 cells. The Neutralization Dose50 (ND50) for anti-mouse osteopontin is approximately 1 to 3 ug/ml in the presence of 2 ug/ml of recombinant mouse osteopontin using 293 cells.
The PA1-25152 immunogen is recombinant, full length mouse protein, expressed in NSO cells.
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Osteopontin is an arginine-glycine-aspartic acid (RGD)-containing glycoprotein that interacts with integrins and CD44 as major receptors. OPN is multifunctional, with activities in cell migration, cell survival, inhibition of calcification, regulation of immune cell function, and control of tumor cell phenotype. Targeting of the gene encoding Osteopontin, has revealed that while it is not necessary for normal embryonic development, fertility, and health under pathogen-free conditions, loss of the protein has significant consequences in several models of injury/disease as diverse as renal injury, viral and bacterial infection, bone remodeling, and tumor growth. The fact that no other proteins seem to share a redundant activity with Osteopontin under these conditions suggests that it has a unique functional role during tissue injury and stress.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Pannexin 3 is a novel target for Runx2, expressed by osteoblasts and mature growth plate chondrocytes.
PA1-25152 was used in immunohistochemistry to study the expression of pannexin-3 in osteoblasts and mature growth plate chondrocytes and its role as a target of Runx2 signaling
|Bond SR,Lau A,Penuela S,Sampaio AV,Underhill TM,Laird DW,Naus CC||Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research (26:2911)||2011|