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Invitrogen
OxPhos Rodent WB Antibody Cocktail
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FIGURE: 1 / 21
OxPhos Rodent WB Antibody (45-8099) in WB
Western blot analysis of OxPhos in Rat liver mitochondria using an OxPhos Monoclonal antibody cocktail (Product # 45-8099). The sample in lane 1 was kept at room temperature, whereas the remaining three samples were heated to 37°C, 50°C, and 100°C, respectively. This blot shows that boiling of samples leads to a decrease in signal due to aggregation of proteins therefore heating samples at or close to boiling is not recommended.
Product Details
45-8099
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Bovine,
Human,
Mouse,
Non-human primate,
Rat
Published species
C. elegans,
Human,
Mouse,
Rat,
Sheep
Host/Isotype
Mouse
/ Ig
Class
Cocktail
Type
Antibody
Clone
Cocktail
Conjugate
Form
Liquid
Concentration
1.5 mg/mL
Purification
purified
Storage buffer
HEPES buffered saline
Contains
0.02% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
This product is a cocktail of multiple OxPhos antibodies.
C-I-20 (ND6) (clone) 20E9DH10C12 - Bovine Complex I
Purification Method: Antigen Affinity Chromatography
C-II-30 (FeS) (clone) 21A11AE7 - Bovine Heart Complex II
Purification Method: IgG Fraction
C-III-Core 2 (clone) 13G12AF12BB11 - Bovine Heart Complex II, III
Purification Method: IgG Fraction
C-IV-I (clone) 1D6E1A8 - Human Complex IV subunit I
Purification Method: Ammonium Sulfate Precipitation
C-V-alpha (clone) 15H4C4 - Bovine Complex V
Purification Method: Antigen Affinity Chromatography
The kit is suitable for Western Blotting analysis of the relative levels of the 5 OxPhos complexes in mitochondrial preparations from mouse, rat, human, or bovine sources. These clones were chosen because they are against a subunit that is labile when its complex is not assembled. Moreover, the combination is readily resolved in SDS-PAGE when the appropriate gel conditions are used. Also, COXI is a highly hydrophobic protein and appears as a broad band at ~35 kDa (not at its true molecular weight at 57 kDa). It is very sensitive to heating. Therefore, the samples, including the positive control, should not be heated over 50°C before loaded on the gel.
The antibody cocktail (1.5 mg/mL) should be diluted 250x to a final working concentration of 6.0 µg/mL for Western blotting. We recommend using PBS with 1% milk as the antibody diluent and using a high pH CAPS/PVDF transfer protocol.
Target Information
Altered levels of assembly can arise from mutations in individual subunits, mutations in assembly factors for the complex(es), mtDNA depletion or as a result of physiological and or pathological changes e.g. hormone treatment, exercise, diet or oxidative stress.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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