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Proteintech
P16-INK4A Polyclonal Antibody
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Product Details
10883-1-AP
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human,
Monkey,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
P16-INK4A Fusion Protein Ag1328 (44-148 aa encoded by BC021998)
Conjugate
Form
Liquid
Concentration
0.37 mg/mL
Amount
55.5 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice (domestic); Dry ice (international)
Product Specific Information
10883-1-AP reacts with p16 specifically. P16 plays an essential role in regulating the cell cycle, and mutations in p16 increase the risk of developing various cancers, including melanoma.
Immunogen sequence: MMMGSARVA ELLLLHGAEP NCADPATLTR PVHDAAREGF LDTLVVLHRA GARLDVRDAW GRLPVDLAEE LGHRDVARYL RAAAGGTRGS NHARIDAAEG PSDIPD (44-148 aa encoded by BC021998)
Target Information
This gene generates several transcript variants which differ in their first exons. At least three alternatively spliced variants encoding distinct proteins have been reported, two of which encode structurally related isoforms known to function as inhibitors of CDK4 kinase. The remaining transcript includes an alternate first exon located 20 Kb upstream of the remainder of the gene; this transcript contains an alternate open reading frame (ARF) that specifies a protein which is structurally unrelated to the products of the other variants. This ARF product functions as a stabilizer of the tumor suppressor protein p53 as it can interact with, and sequester, the E3 ubiquitin-protein ligase MDM2, a protein responsible for the degradation of p53. In spite of the structural and functional differences, the CDK inhibitor isoforms and the ARF product encoded by this gene, through the regulatory roles of CDK4 and p53 in cell cycle G1 progression, share a common functionality in cell cycle G1 control. This gene is frequently mutated or deleted in a wide variety of tumors, and is known to be an important tumor suppressor gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: a frameshift between exon 1 (0.18) and exon 2 changed the ORF of p16INK4 gene.; Alternative reading frame; ARF; CDK4 inhibitor p16 INK4; CDK4 inhibitor p16-INK4; CDK4I; CDKN2A/ARF Intron 2 lncRNA; cell cycle negative regulator beta; Cyclin dependent kinase 4 inhibitor A; Cyclin-dependent kinase 4 inhibitor A; Cyclin-dependent kinase inhibitor 2A; cyclin-dependent kinase inhibitor 2A (melanoma, p16, inhibits CDK4); HGNC:1787; hypothetical 18.1 kDa protein from longest open reading frame; inhibitor of cdk4 A; inhibits CDK4; isoforms 1/2/3; Melanoma p16 inhibits CDK4; MTS-1; Multiple tumor suppressor 1; multiple tumour suppressor 1; P14 P16; p14ARF; p16; p16 gamma; p16-INK4; p16-INK4a; P19 TP16; Tumor suppressor ARF
Gene Aliases: ARF; CAI2; CDK4I; CDKN2; CDKN2A; CMM2; INK4; INK4A; MLM; MTS-1; MTS1; P14; P14ARF; P16; P16-INK4A; P16INK4; P16INK4A; P19; P19ARF; TP16
UniProt ID: (Human) P42771
Entrez Gene ID: (Human) 1029
p53 binding
DNA binding
RNA binding
cyclin-dependent protein serine/threonine kinase inhibitor activity
protein binding
SUMO transferase activity
protein kinase binding
NF-kappaB binding
ligase inhibitor activity
ubiquitin-protein transferase inhibitor activity
RNA polymerase II sequence-specific DNA binding transcription factor binding
MDM2/MDM4 family protein binding
disordered domain specific binding
ubiquitin ligase inhibitor activity
negative regulation of transcription from RNA polymerase II promoter
protein polyubiquitination
mitophagy
negative regulation of cell-matrix adhesion
rRNA processing
apoptotic process
Ras protein signal transduction
negative regulation of cell proliferation
epidermis development
rRNA transcription
regulation of gene expression
positive regulation of gene expression
protein sumoylation
keratinocyte differentiation
negative regulation of cell growth
nuclear body organization
negative regulation of B cell proliferation
regulation of protein stability
protein destabilization
negative regulation of immature T cell proliferation in thymus
positive regulation of protein sumoylation
mammary gland epithelial cell proliferation
negative regulation of mammary gland epithelial cell proliferation
positive regulation of smooth muscle cell apoptotic process
protein localization to nucleus
somatic stem cell population maintenance
glucose homeostasis
positive regulation of apoptotic process
positive regulation of DNA damage response, signal transduction by p53 class mediator
keratinocyte proliferation
negative regulation of cyclin-dependent protein serine/threonine kinase activity
negative regulation of cell cycle
negative regulation of transcription, DNA-templated
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
regulation of nucleocytoplasmic transport
regulation of protein export from nucleus
somatic stem cell division
protein stabilization
regulation of cell cycle
mitochondrial depolarization
apoptotic process involved in mammary gland involution
positive regulation of apoptotic process involved in mammary gland involution
cellular response to hydrogen peroxide
protein K63-linked ubiquitination
cellular senescence
replicative senescence
oncogene-induced cell senescence
apoptotic signaling pathway
positive regulation of protein localization to nucleus
positive regulation of signal transduction by p53 class mediator
protein localization to nucleolus
negative regulation of proteolysis involved in cellular protein catabolic process
regulation of protein targeting to mitochondrion
negative regulation of ubiquitin protein ligase activity
amyloid fibril formation
regulation of G1/S transition of mitotic cell cycle
negative regulation of protein ubiquitination involved in ubiquitin-dependent protein catabolic process
positive regulation of macrophage apoptotic process
negative regulation of protein neddylation
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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