Proteintech

P21 Polyclonal Antibody

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P21 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of ( 4% PFA) fixed MCF-7 cells using 10355-1-AP (P21;CDKN1A antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

P21 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human cervical cancer tissue slide using 10355-1-AP ( P21;CDKN1A Antibody) at dilution of 1:400 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

P21 Antibody in Western Blot (WB) — HEK-293T cells were subjected to SDS PAGE followed by western blot with 10355-1-AP (P21;CDKN1A antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

P21 Antibody in Flow Cytometry (Flow) — 1X10^6 HeLa cells were stained with 0.2ug P21;CDKN1A antibody (10355-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L) with dilution 1:1500.

P21 Antibody in Immunoprecipitation (IP) — IP result of anti-P21;CDKN1A (IP:10355-1-AP, 4ug; Detection:10355-1-AP 1:1000) with MCF-7 cells lysate 2800 ug.

Product Details

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 119 publications 119 publications

Immunohistochemistry (IHC)

View 7 publications 7 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:500

Immunocytochemistry (ICC/IF)

1:50-1:500

View 4 publications 4 publications

Flow Cytometry (Flow)

0.2 µg/1x10^6 cells

View 1 publication 1 publication

Immunoprecipitation (IP)

0.5-4.0 µg

Miscellaneous PubMed (Misc)

View 7 publications 7 publications

Product Specifications

Species Reactivity

Human

Published species

Arthropod, Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

P21 Fusion Protein Ag0368

Conjugate

Unconjugated

Form

Liquid

Concentration

0.39 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Target Information

Protein p21 is a tumor suppressor protein which is an inhibitor of Cyclin-dependent kinases (CDK) and is transcriptionally activated by p53. P21 is important in the response of cells to genotoxic stress and a major transcriptional target of p53 protein. The occurrence of p21 in the nucleus executes binding and inhibition activity of cyclin dependent kinases Cdk1 and Cdk2, and blocks the transition from G1 phase into S phase or from G2 phase into mitosis after DNA damage, which enables the repair of damaged DNA. In the cytoplasm, p21 protein has an anti-apoptotic effect. P21 is able to bind to and inhibit caspase 3, as well as the apoptotic kinases ASK1 and JNK. P21 exhibits a dual function in carcinogenesis, and acts as a tumor suppressor, prevents apoptosis, and acts as an oncogene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: CDK-interacting protein 1; CDK-interaction protein 1; CDNK1A; Cyclin-dependent kinase inhibitor 1; cyclin-dependent kinase inhibitor 1A (p21, Cip1); DNA synthesis inhibitor; MDA-6; melanoma differentiation associated protein 6; Melanoma differentiation-associated protein 6; OTTHUMP00000016298; p21; p21Cip1/Waf1; wild-type p53-activated fragment 1

Gene Aliases: CAP20; CDKN1; CDKN1A; CIP1; MDA-6; MDA6; P21; p21CIP1; PIC1; SDI1; WAF1

UniProt ID: (Human) P38936

Entrez Gene ID: (Human) 1026

Molecular Function: protein-binding activity modulator kinase inhibitor kinase modulator