Invitrogen

P2X7 Polyclonal Antibody

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P2X7 Antibody in Western Blot (WB) — PA5-19165 (0.3 µg/mL) in Human Brain (Frontal Cortex) lysate with (B) and without (A) blocking with the immunising peptide, the primary antibody incubation was 1 hour and detected by chemiluminescence.

Product Details

Tested Applications

Dilution

Immunohistochemistry (IHC)

3.75 µg/mL

Immunohistochemistry (Paraffin) (IHC (P))

3-6 µg/mL

Western Blot (WB)

1-3 µg/mL

Product Specifications

Species Reactivity

Human

Host / Isotype

Goat / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide sequence (YETNKVTRIQSMNY) corresponding to the N-terminus amino acids of P2RX7

Conjugate

Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

TBS, pH 7.3, with 0.5% BSA

Contains

0.02% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

RRID

AB_10979297

Product Specific Information

This antibody is predicted to react with canine, mouse and rat based on sequence homology.

Target Information

The product of this gene belongs to the family of purinoceptors for ATP. This receptor functions as a ligand-gated ion channel and is responsible for ATP-dependent lysis of macrophages through the formation of membrane pores permeable to large molecules. Activation of this nuclear receptor by ATP in the cytoplasm may be a mechanism by which cellular activity can be coupled to changes in gene expression. Multiple alternatively spliced variants have been identified, most of which fit nonsense-mediated decay criteria.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: ATP receptor; MGC20089; P2RX7; P2X purinoceptor 7; P2X7; P2X7 receptor; P2Z receptor; Purinergic receptor; purinergic receptor P2X, ligand gated ion channel, 7; purinergic receptor P2X, ligand-gated ion channel, 7; purinergic receptor P2X7; purinergic receptor P2X7 variant A

Gene Aliases: P2RX7; P2X7

UniProt ID: (Human) Q99572

Entrez Gene ID: (Human) 5027

Molecular Function: ion channel ligand-gated ion channel receptor transporter

activation of MAPK activity cell morphogenesis phagolysosome assembly positive regulation of T cell mediated cytotoxicity regulation of sodium ion transport protein phosphorylation membrane protein ectodomain proteolysis phospholipid transfer to membrane membrane budding inflammatory response mitochondrion organization cell surface receptor signaling pathway blood coagulation response to mechanical stimulus response to zinc ion positive regulation of calcium ion transport into cytosol positive regulation of gene expression positive regulation of glutamate secretion positive regulation of gamma-aminobutyric acid secretion synaptic vesicle exocytosis protein processing phospholipid scrambling sensory perception of pain cytolysis positive regulation of bone mineralization cellular response to extracellular stimulus bleb assembly positive regulation of prostaglandin secretion response to lipopolysaccharide positive regulation of interleukin-6 production collagen metabolic process response to ATP response to fluid shear stress positive regulation of ion transmembrane transport purinergic nucleotide receptor signaling pathway T cell proliferation response to drug T cell homeostasis NAD transport negative regulation of MAPK cascade multicellular organismal protein catabolic process phospholipid translocation negative regulation of bone resorption negative regulation of cell volume positive regulation of glycolytic process positive regulation of cytolysis ceramide biosynthetic process pore complex assembly skeletal system morphogenesis homeostasis of number of cells within a tissue positive regulation of interleukin-1 alpha secretion positive regulation of interleukin-1 beta secretion defense response to Gram-positive bacterium release of sequestered calcium ion into cytosol protein oligomerization positive regulation of cytoskeleton organization response to calcium ion response to electrical stimulus regulation of killing of cells of other organism membrane depolarization positive regulation of mitochondrial depolarization positive regulation of lymphocyte apoptotic process cellular response to dsRNA reactive oxygen species metabolic process apoptotic signaling pathway extrinsic apoptotic signaling pathway positive regulation of bleb assembly

Process(es)

lipopolysaccharide binding purinergic nucleotide receptor activity extracellular ATP-gated cation channel activity receptor binding protein binding ATP binding protein homodimerization activity