Invitrogen

P2X7 Polyclonal Antibody

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P2X7 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of P2X7 in paraffin embedded human cortex using a P2X7 polyclonal antibody (Product #PA5-19165) at a concentration of 3.8 µg/mL. Steamed antigen retrieval was performed with pH 6 buffered citrate. Samples were then stained with alkaline phosphatase.

P2X7 Antibody in Western Blot (WB) — PA5-19165 (0.3 µg/mL) in Human Brain (Frontal Cortex) lysate with (B) and without (A) blocking with the immunising peptide, the primary antibody incubation was 1 hour and detected by chemiluminescence.

P2X7 Antibody in Immunohistochemistry (IHC) — Fig 1 P2RX7 and inflammasome activation are increased in NASH-affected liver. (A) Representative images (objective 2X) of Sirius red staining (top) and immunohistochemical staining (objective 40X) of P2RX7 (bottom) in liver tissue from a representative control and NASH-affected donor with quantification of Sirius red + area and P2RX7 + cells ( n = 5 individuals per group; n = 6-8 images per donor). Scale bar, 100 muM. Black arrows highlight P2RX7 + cells. (B) Quantification of CD45 + , CD45 + / P2RX7 + , CD14 + , CD14 + / P2RX7 + , CD68 + , CD68 + / P2RX7 + cells ( n = 5 individuals per group; n = 6-8 images per donor). (C) IL-1beta, (D) caspase-1, (E) CCL2, and (F) CCL5 levels in liver tissue from control and NASH-affected liver biopsies ( n = 5 individuals per group). (G) Relative expression levels of P2RX 7, NLRP3 , AIM2 , ASC , CASP1 , IL-1beta and (H) CD11b , CD45 , Tnfalpha , Il-6 , CCL2 , CCL5 in liver tissue from control and NASH-affected donors ( n = 5 individuals per group). n.d., for not detected. In all statistical plots, the data are shown as the mean +- SEM. * P <= 0.05, ** P <= 0.01, *** P <= 0.001, **** P <= 0.0001 by two-sided Student's t-test.

Product Details

PA5-19165

Applications	Tested Dilution	Publications
Western Blot (WB)	1-3 µg/mL	-
Immunohistochemistry (IHC)	3.75 µg/mL	View 1 publication 1 publication
Immunohistochemistry (Paraffin) (IHC (P))	3-6 µg/mL	-

Product Specifications
Species Reactivity	Human
Published species	Human
Host/Isotype	Goat / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Synthetic peptide sequence (YETNKVTRIQSMNY) corresponding to the N-terminus amino acids of P2RX7 View antigen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.5 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	TBS, pH 7.3, with 0.5% BSA
Contains	0.02% sodium azide
Storage conditions	-20° C, Avoid Freeze/Thaw Cycles
RRID	AB_10979297

Product Specific Information

This antibody is predicted to react with canine, mouse and rat based on sequence homology.

Target Information

The product of this gene belongs to the family of purinoceptors for ATP. This receptor functions as a ligand-gated ion channel and is responsible for ATP-dependent lysis of macrophages through the formation of membrane pores permeable to large molecules. Activation of this nuclear receptor by ATP in the cytoplasm may be a mechanism by which cellular activity can be coupled to changes in gene expression. Multiple alternatively spliced variants have been identified, most of which fit nonsense-mediated decay criteria.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Pharmacological inhibition of P2RX7 ameliorates liver injury by reducing inflammation and fibrosis.

PloS one

Baeza-Raja B,Goodyear A,Liu X,Lam K,Yamamoto L,Li Y,Dodson GS,Takeuchi T,Kisseleva T,Brenner DA,Dabbagh K

PA5-19165 was used in Immunohistochemistry to report that P2RX7 is expressed by infiltrating monocytes and resident Kupffer cells in livers from NASH-affected individuals.

Fri Aug 21 00:00:00 EDT 2020

Pharmacological inhibition of P2RX7 ameliorates liver injury by reducing inflammation and fibrosis.

PloS one

Baeza-Raja B,Goodyear A,Liu X,Lam K,Yamamoto L,Li Y,Dodson GS,Takeuchi T,Kisseleva T,Brenner DA,Dabbagh K

PA5-19165 was used in Immunohistochemistry to report that P2RX7 is expressed by infiltrating monocytes and resident Kupffer cells in livers from NASH-affected individuals.

Fri Aug 21 00:00:00 EDT 2020

Bioinformatics

Protein Aliases: ATP receptor; MGC20089; P2X purinoceptor 7; p2X receptor 7; P2X7; P2X7 receptor; P2Z; P2Z receptor; Purinergic receptor; purinergic receptor P2X, ligand gated ion channel, 7; purinergic receptor P2X, ligand-gated ion channel, 7; purinergic receptor P2X7 variant A

Gene Aliases: P2RX7; P2X7

UniProt ID: (Human) Q99572

Entrez Gene ID: (Human) 5027

Molecular Function: ligand-gated ion channel