Proteintech

P3H3 Polyclonal Antibody

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P3H3 Antibody (16023-1-AP) in WB

MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with 16023-1-AP (P3H3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

P3H3 Antibody in Immunoprecipitation (IP)

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Product Details

16023-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

View 6 publications 6 publications

Immunoprecipitation (IP)

0.5-4.0 µg

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

P3H3 Fusion Protein Ag8850 (223-605 aa encoded by BC017217)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.16 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: RALNQYQAQ LGEPRPGLGP REDIQRFILR SLGEKRQLYY AMEHLGTSFK DPDPWTPAAL IPEALREKLR EDQEKRPWDH EPVKPKPLTY WKDVLLLEGV TLTQDSRQLN GSERAVLDGL LTPAECGVLL QLAKDAAGAG ARSGYRGRRS PHTPHERFEG LTVLKAAQLA RAGTVGSQGA KLLLEVSERV RTLTQAYFSP ERPLHLSFTH LVCRSAIEGE QEQRMDLSHP VHADNCVLDP DTGECWREPP AYTYRDYSGL LYLNDDFQGG DLFFTEPNAL TVTARVRPRC GRLVAFSSGV ENPHGVWAVT RGRRCALALW HTWAPEHREQ EWIEAKELLQ ESQEEEEEEE EEMPSKDPSP EPPSRRHQRV QDKTGRAPRV REEL (223-605 aa encoded by B C017217)

Target Information

The protein encoded by this gene belongs to the leprecan family of proteoglycans, which function as collagen prolyl hydroxylases that are required for proper collagen biosynthesis, folding and assembly. This protein, like other family members, is thought to reside in the endoplasmic reticulum. Epigenetic inactivation of this gene is associated with breast and other cancers, suggesting that it may function as a tumor suppressor.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Type I and type V procollagen triple helix uses different subsets of the molecular ensemble for lysine posttranslational modifications in the rER.

The Journal of biological chemistry

Ishikawa Y,Taga Y,Zientek K,Mizuno N,Salo AM,Semenova O,Tufa SF,Keene DR,Holden P,Mizuno K,Gould DB,Myllyharju J,Bächinger HP

Mon Aug 30 00:00:00 UTC 2021

Cyclophilin B control of lysine post-translational modifications of skin type I collagen.

PLoS genetics

Terajima M,Taga Y,Cabral WA,Liu Y,Nagasawa M,Sumida N,Kayashima Y,Chandrasekaran P,Han L,Maeda N,Perdivara I,Hattori S,Marini JC,Yamauchi M

Sat Jun 01 00:00:00 UTC 2019

Expression characterization and functional implication of the collagen-modifying Leprecan proteins in mouse gonadal tissue and mature sperm.

AIMS genetics

Zimmerman SM,Besio R,Heard-Lipsmeyer ME,Dimori M,Castagnola P,Swain FL,Gaddy D,Diekman AB,Morello R

Wed Sep 30 00:00:00 UTC 2020

P3h3-null and Sc65-null Mice Phenocopy the Collagen Lysine Under-hydroxylation and Cross-linking Abnormality of Ehlers-Danlos Syndrome Type VIA.

The Journal of biological chemistry

Hudson DM,Weis M,Rai J,Joeng KS,Dimori M,Lee BH,Morello R,Eyre DR

Fri Mar 03 00:00:00 UTC 2017

P3h3-null and Sc65-null Mice Phenocopy the Collagen Lysine Under-hydroxylation and Cross-linking Abnormality of Ehlers-Danlos Syndrome Type VIA.

The Journal of biological chemistry

Hudson DM,Weis M,Rai J,Joeng KS,Dimori M,Lee BH,Morello R,Eyre DR

Fri Mar 03 00:00:00 UTC 2017

Sc65-Null Mice Provide Evidence for a Novel Endoplasmic Reticulum Complex Regulating Collagen Lysyl Hydroxylation.

PLoS genetics

Heard ME,Besio R,Weis M,Rai J,Hudson DM,Dimori M,Zimmerman SM,Kamykowski JA,Hogue WR,Swain FL,Burdine MS,Mackintosh SG,Tackett AJ,Suva LJ,Eyre DR,Morello R

Fri Apr 01 00:00:00 UTC 2016

Sc65-Null Mice Provide Evidence for a Novel Endoplasmic Reticulum Complex Regulating Collagen Lysyl Hydroxylation.

PLoS genetics

Heard ME,Besio R,Weis M,Rai J,Hudson DM,Dimori M,Zimmerman SM,Kamykowski JA,Hogue WR,Swain FL,Burdine MS,Mackintosh SG,Tackett AJ,Suva LJ,Eyre DR,Morello R

Fri Apr 01 00:00:00 UTC 2016

Sc65-Null Mice Provide Evidence for a Novel Endoplasmic Reticulum Complex Regulating Collagen Lysyl Hydroxylation.

PLoS genetics

Heard ME,Besio R,Weis M,Rai J,Hudson DM,Dimori M,Zimmerman SM,Kamykowski JA,Hogue WR,Swain FL,Burdine MS,Mackintosh SG,Tackett AJ,Suva LJ,Eyre DR,Morello R

Fri Apr 01 00:00:00 UTC 2016

Sc65-Null Mice Provide Evidence for a Novel Endoplasmic Reticulum Complex Regulating Collagen Lysyl Hydroxylation.

PLoS genetics

Heard ME,Besio R,Weis M,Rai J,Hudson DM,Dimori M,Zimmerman SM,Kamykowski JA,Hogue WR,Swain FL,Burdine MS,Mackintosh SG,Tackett AJ,Suva LJ,Eyre DR,Morello R

Fri Apr 01 00:00:00 UTC 2016

Glyoxalase 1-knockdown in human aortic endothelial cells - effect on the proteome and endothelial function estimates.

Scientific reports

Stratmann B,Engelbrecht B,Espelage BC,Klusmeier N,Tiemann J,Gawlowski T,Mattern Y,Eisenacher M,Meyer HE,Rabbani N,Thornalley PJ,Tschoepe D,Poschmann G,Stühler K

Tue Nov 29 00:00:00 UTC 2016

Glyoxalase 1-knockdown in human aortic endothelial cells - effect on the proteome and endothelial function estimates.

Scientific reports

Stratmann B,Engelbrecht B,Espelage BC,Klusmeier N,Tiemann J,Gawlowski T,Mattern Y,Eisenacher M,Meyer HE,Rabbani N,Thornalley PJ,Tschoepe D,Poschmann G,Stühler K

Tue Nov 29 00:00:00 UTC 2016

Bioinformatics

Protein Aliases: gene rich cluster, B; leprecan-like 2; Leprecan-like protein 2; procollagen-proline 3-dioxygenase; Prolyl 3-hydroxylase 3; Protein B

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Gene Aliases: BC016431; GRCB; HSU47926; LEPREL2; P3H3

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UniProt ID: (Human) Q8IVL6, (Mouse) Q8CG70

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Entrez Gene ID: (Human) 10536, (Rat) 297595, (Mouse) 14789

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Function(s)

iron ion binding procollagen-proline 3-dioxygenase activity L-ascorbic acid binding molecular_function oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, 2-oxoglutarate as one donor, and incorporation of one atom each of oxygen into both donors oxidoreductase activity oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen metal ion binding dioxygenase activity extracellular matrix glycoprotein

Process(es)

negative regulation of cell proliferation peptidyl-proline hydroxylation oxidation-reduction process biological_process

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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P3H3 Polyclonal Antibody

P3H3 Antibody (16023-1-AP) in WB

Product Details

16023-1-AP

Western Blot (WB)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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