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Identifyn
RAD51 SRM Recombinant Rabbit Monoclonal Antibody (BLR017M), Identifyn™
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FIGURE: 1 / 8
RAD51 SRM Antibody (PA-RR000002) in ICC/IF
IdentifynTM Detailed protocols can be found below under Documents & Downloads. Airyscan: HeLa Cells were grown on acid-etched 1.5H cover glass to ~50% confluency. DNA damage was induced via treatment with 2 µM Etoposide–spiked complete growth media for 24 hours, followed by -20°C methanol fixation and blocked with Thermo Fisher Fish Serum Blocker (Product # 37527X3). The primary antibody, Rabbit anti-Human ... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA-RR000002
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Expression System
ExpiCHO
Class
Recombinant Monoclonal
Type
Antibody
Clone
BLR017M
Immunogen
Between 315 and C-terminus.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
50 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 10% glycerol
Contains
0.02% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Identifyn™ Primary and Secondary antibodies are extensively validated in Immunofluorescence assays by traditional as well as high resolution acquisition like Widefield SIM, Confocal, Airyscan and STORM Microscopy.
Recommended dilution for traditional microscopy:
Widefield - 1:500
Widefield SIM - 1:500
Confocal - 1:500
Confocal NIR - 1:500
Recommended dilution for Super Resolution microscopy:
SIM (Structured Illumination Microscopy) - 1:500
Single Molecule STORM (Stochastic Optical Reconstruction Microscopy) - 1:200
Detailed protocols can be found below under Methods & Protocols.
Target Information
RAD51 plays a critical role in homologous strand exchange, a key step in DNA repair through homologous recobination. It binds to single and double-stranded DNA and exhibits DNA-dependent ATPase activity. RAD51 catalyzes the recognition of homology and strand exchange between homologous DNA partners to form a joint molecule between a processed DNA break and the repair template. It binds to a single-stranded DNA in an ATP-dependent manner to form nucleoprotein filaments which are essential for the homology search and strand exchange. Mutations in the gene can results in breast cancer, mirror movements 2 and fanconi anemia.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: BRCA1/BRCA2-containing complex, subunit 5; RAD51; RAD51 homolog A; RecA, E. coli, homolog of; RecA-like protein; recombination protein A; unnamed protein product
Gene Aliases: BRCC5; FANCR; HRAD51; HsRad51; HsT16930; MRMV2; RAD51A; RECA
UniProt ID: (Human) Q06609
Entrez Gene ID: (Human) 5888
recombinase activity
nucleotide binding
DNA binding
chromatin binding
double-stranded DNA binding
single-stranded DNA binding
protein binding
ATP binding
DNA-dependent ATPase activity
ATPase activity
enzyme binding
nucleosome binding
identical protein binding
DNA polymerase binding
ATP-dependent DNA damage sensor activity
telomere maintenance via recombination
double-strand break repair via homologous recombination
DNA recombinase assembly
DNA metabolic process
DNA repair
DNA recombination
mitotic recombination
cellular response to DNA damage stimulus
meiosis I
reciprocal meiotic recombination
response to xenobiotic stimulus
response to toxic substance
response to X-ray
regulation of double-strand break repair via homologous recombination
telomere maintenance via telomere lengthening
replication fork processing
interstrand cross-link repair
strand invasion
meiotic cell cycle
chromosome organization involved in meiotic cell cycle
cellular response to ionizing radiation
cellular response to gamma radiation
cellular response to cisplatin
cellular response to camptothecin
response to glucoside
mitotic recombination-dependent replication fork processing
double-strand break repair involved in meiotic recombination
regulation of DNA damage checkpoint
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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