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Invitrogen
FKBP12 Polyclonal Antibody
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FIGURE: 1 / 25
FKBP12 Antibody (PA1-026A) in IHC (P)
Immunohistochemistry was performed on cancer biopsies of deparaffinized human Colon carcinoma tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:200 with a rabbit poly... View More
Product Details
PA1-026A
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Published species
Human,
Mouse,
Pig,
Protozoa,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide corresponding to the N-terminal residues G(1) V Q V E T I S P G D G R(13) of human FKBP12.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS with 1mg/mL BSA
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
PA1-026A detects FK506 binding protein 12 kDa (FKBP12) in human and mouse tissues.
PA1-026A has been successfully used in Western blot, immunohistochemistry, and immunoprecipitation procedures. By Western blot, this antibody detects a 12 kDa protein representing FKBP12 from rat heart homogenate.
The PA1-026A immunogen is a synthetic peptide corresponding to the N-terminal residues G(1) V Q V E T I S P G D G R(13) of human FKBP12. PA1-026A immunizing peptide (Cat. # PEP-012) is available for use in neutralization and control experiments.
Target Information
FKBP12 is a member of the immunophilin protein family, which play a role in immunoregulation and basic cellular processes involving protein folding and trafficking. The protein is a cis-trans prolyl isomerase that binds the immunosuppressants FK506 and rapamycin. It interacts with several intracellular signal transduction proteins including type I TGF-beta receptor. It also interacts with multiple intracellular calcium release channels, and coordinates multi-protein complex formation of the tetrameric skeletal muscle ryanodine receptor. In mouse, deletion of this homologous gene causes congenital heart disorder known as noncompaction of left ventricular myocardium.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: 12 kDa FK506-binding protein; 12 kDa FKBP; Calstabin-1; EC 5.2.1.8; FK506 binding protein 1A, 12kDa; FK506 binding protein12; FK506-binding protein (FKBP); FK506-binding protein 1; FK506-binding protein 12; FK506-binding protein 1A; FK506-binding protein, T-cell, 12-kD; FKBP-1A; FKBP12-Exip3; FKBP1A; Immunophilin FKBP12; Peptidyl-prolyl cis-trans isomerase FKBP1A; PPIase FKBP1A; protein kinase C inhibitor 2; Rotamase
Gene Aliases: Fkbp; FKBP-12; FKBP-1A; FKBP1; FKBP12; FKBP1A; PKC12; PKCI2; PPIASE
UniProt ID: (Human) P62942, (Mouse) P26883
Entrez Gene ID: (Human) 2280, (Mouse) 14225
peptidyl-prolyl cis-trans isomerase activity
transforming growth factor beta receptor binding
calcium channel regulator activity
protein binding
macrolide binding
FK506 binding
isomerase activity
receptor inhibitor activity
type I transforming growth factor beta receptor binding
ion channel binding
I-SMAD binding
activin receptor binding
chaperone
heart morphogenesis
protein folding
'de novo' protein folding
regulation of cardiac muscle contraction by regulation of the release of sequestered calcium ion
regulation of skeletal muscle contraction by regulation of release of sequestered calcium ion
negative regulation of transforming growth factor beta receptor signaling pathway
regulation of protein localization
negative regulation of activin receptor signaling pathway
protein refolding
T cell activation
positive regulation of I-kappaB kinase/NF-kappaB signaling
regulation of immune response
protein maturation
ventricular cardiac muscle tissue morphogenesis
heart trabecula formation
fibril organization
regulation of amyloid precursor protein catabolic process
amyloid fibril formation
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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