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Invitrogen
Furin Polyclonal Antibody
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FIGURE: 1 / 41
Furin Antibody (PA1-062) in ICC/IF
Immunofluorescence analysis of Furin was performed using 70% confluent log phase BeWo cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with Furin Polyclonal Antibody (Product # PA1-062) at 2 µg/mL dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A327... View More
Product Details
PA1-062
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Dog,
Human,
Mouse,
Rat
Published species
Dog,
Hamster,
Human,
Mouse,
Non-human primate,
Pig,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide corresponding to residues R(780) G E R T A F I K D Q S A L(793) of human Furin.
Conjugate
Form
Liquid
Concentration
2 mg/mL
Amount
200 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS with 1mg/mL BSA
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
PA1-062 detects furin convertase from canine and mouse cells as well as transfected human furin. This antibody does not detect endogenous furin from BSC-40, HeLa, J774A.1 BPAEC, or CHO cells, or from rat skeletal muscle, spleen, kidney, ovary, testes, heart, or brain tissue.
PA1-062 has been successfully used in Western blot, immunocytochemistry, immunoprecipitation, and immunofluorescence procedures. By Western blot, this antibody detects an ~100 kDa protein representing furin in BSC-40 cells transfected with the human furin gene. In addition, PA1-062 detects human furin transfected into 3T3 cells. Immunofluorescence staining of endogenous furin convertase in 3T3 cells with PA1-062 yields a pattern consistent with paranuclear staining.
The PA1-062 immunogen is a synthetic peptide corresponding to residues R(780) G E R T A F I K D Q S A L(793) of human Furin. The PA1-062 immunizing peptide (Cat. # PEP-010) is available for use in neutralization and control experiments.
Target Information
Furin is a membrane-associated, calcium-dependent, serine protease that belongs to the subtilisin-like prohormone convertase (PC) family. Members of this family of cellular enzymes cleave most prohormones and neuropeptide precursors. Numerous other cellular proteins, some viral proteins, and bacterial toxins that are transported by the constitutive secretory pathway are also targeted for maturation by PCs. Furin and other PC family members share structural similarities which include a heterogeneous ~10 kDa amino-terminal proregion, a highly conserved ~55 kDa subtilisin-like catalytic domain, and carboxyl-terminal domain that is heterogeneous in length and sequence. These enzymes become catalytically active following proregion cleavage within the appropriate cellular compartment. Furin is the only known PC to possess a transmembrane domain. Cleavage of target proteins occurs at the carboxyl-terminus of the furin consensus sequence, RX(K/R)R. It has been shown that the acidic peptide sequence, C771PSDSEEDEG780, localizes furin to the trans-Golgi-network. Phosphorylation of serine residues within this region modulates intracellular routing of furin protein. An additional signaling domain includes the tetrapeptide sequence, Y759KGL762, which directs internalization from the cell surface.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: dibasic processing enzyme; Dibasic-processing enzyme; FES upstream region; Furin; furin, membrane associated receptor protein; PACE; Paired basic amino acid cleaving enzyme (furin); paired basic amino acid residue cleaving enzyme; Paired basic amino acid residue-cleaving enzyme; Prohormone convertase 3; proprotein convertase subtilisin/kexin 3; proprotein convertase subtilisin/kexin type 3; Proprotein convertase subtilisin/kexin type 3 (paired basic amino acid cleaving enzyme, furin, membrane associated receptor protein); proprotein convertase subtilisin/kexin type3; subtilisin pro-protein processing enzyme; subtilisin-like proprotein convertase 1; unnamed protein product
Gene Aliases: 9130404I01Rik; FUR; FURIN; PACE; PCSK3; SPC1
UniProt ID: (Human) P09958, (Rat) P23377, (Mouse) P23188
Entrez Gene ID: (Dog) 488746, (Human) 5045, (Rat) 54281, (Mouse) 18550
protease binding
endopeptidase activity
serine-type endopeptidase activity
serine-type endopeptidase inhibitor activity
protein binding
heparin binding
peptidase activity
serine-type peptidase activity
hydrolase activity
peptide binding
metal ion binding
nerve growth factor binding
endopeptidase activator activity
heparan sulfate binding
serine protease
blastocyst formation
negative regulation of inflammatory response to antigenic stimulus
signal peptide processing
proteolysis
transforming growth factor beta receptor signaling pathway
regulation of signal transduction
protein processing
peptide hormone processing
viral life cycle
viral translation
viral protein processing
extracellular matrix disassembly
extracellular matrix organization
collagen catabolic process
zymogen activation
regulation of cholesterol transport
negative regulation of low-density lipoprotein particle receptor catabolic process
nerve growth factor production
negative regulation of nerve growth factor production
negative regulation of transforming growth factor beta1 production
secretion by cell
plasma lipoprotein particle remodeling
regulation of protein catabolic process
peptide biosynthetic process
positive regulation of viral entry into host cell
viral entry into host cell
positive regulation of membrane protein ectodomain proteolysis
protein maturation
dibasic protein processing
zymogen inhibition
cytokine precursor processing
amyloid fibril formation
aging
cell proliferation
negative regulation of endopeptidase activity
positive regulation of cell migration
positive regulation of transforming growth factor beta receptor signaling pathway
regulation of low-density lipoprotein particle receptor biosynthetic process
regulation of endopeptidase activity
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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